Ab108383

AIN93-G standard and modified diet was purchased from Trophic Animal Feed High-Tech Company, Ltd. (Nantong, China). Lactoferrin of milk origin was purchased from Hilmar Cheese Company (CA, USA). The reagents, as well as molecular weight marker and nitrocellulose membranes for sodium dodecyl sulphate–polyacrylamide gel electrophoresis (SDS-PAGE) were purchased from Beyotime Institute of Technology (Jiangsu, China) and Bio-Rad (CA, USA), respectively. The antibodies were obtained from ImmunoWay Biotechnology Company (DE, USA) as follows: phosphorylation of Tau at serine 396 (p-Tau serine396) (YP0263), p-Tau serine404 (YP0264). Anti-post synaptic density protein-95 (PSD95) (AJ1661a) was from Abgent (SD, USA). Antibodies directly against β-site APP cleaving enzyme 1 (BACE1) (5606), synaptophysin (5461), glial fibrillary acidic protein (GFAP) (3670) ,and β-Actin (4970) were from Cell Signaling (MA, USA). These following antibodies were obtained from Abcam (Shanghai, China): insulin degrading enzyme (IDE) (ab32216), cathepsin B (ab58802), and brain derived neurotrophic factor (BDNF) (ab108383). An antibody against ionized calcium binding adaptor molecule 1 (Ibα1) (016-20001) was from Wako (Osaka, Japan).

For western blot analysis, anti-SOX2 (#3579, 1:1000, Cell Signaling Technology), anti-MYC (#5605, 1:1000, Cell Signaling Technology), anti-BDNF (ab108383, 1:1000, Abcam), anti-NGF (ab52918, 1:1000, Abcam), and anti-β-actin (#4967, 1:2000, Cell Signaling Technology) were used as the primary antibodies. HRP-linked anti-mouse IgG (#7076, 1:1000, Cell Signaling Technology) and HRP-linked anti-rabbit IgG (#7074, 1:1000, Cell Signaling Technology) antibodies were used as secondary antibodies. Uncropped scans of the blots are provided in Supplementary Fig. 11.

Sections were incubated for BDNF (ab108383, Abcam’sRabMAb® technology, USA), TrkB (ab33655, Abcam’sRabMAb® technology USA), and TrkC (ab33656, Abcam’sRabMAb® technology, USA).