MO leaf aqueous extract (10 % w/v) was made by soaking 10 g of crushed leaves in one hundred mL of DW for 24 h with stirring by an orbital shaker (Thermo Fisher Scientific, Inc). The extract underwent filtration using Whatman No. 4 filter paper, and centrifuged for 15 min at 5000 rpm; the crude extract was obtained using lyophilization and afterward preserved at a temperature of 4 °C until it was ready for use [30 ,42 (link)].
Orbital shaker
Manufactured by Thermo Fisher Scientific
Sourced in United States, India, Germany
The Orbital Shaker is a versatile laboratory equipment designed to mix and agitate samples in a gentle, consistent manner. The core function of this product is to create a circular, orbital motion that facilitates the uniform suspension and mixing of liquids, cells, or other materials within various containers, such as flasks, beakers, or microplates.
Automatically generated - may contain errors
Lab products found in correlation
Freestyle 293 expression medium, by Thermo Fisher Scientific (3 mentions)
Versa star pro, by Thermo Fisher Scientific (2 mentions)
T175 flask, by Sarstedt (2 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (2 mentions)
Whatman no 4 filter paper, by Cytiva (1 mentions)
Whatman 1 filter paper, by Cytiva (1 mentions)
Fluoroskan ascent fl microplate luminometer, by Thermo Fisher Scientific (1 mentions)
D8 advance, by Brucker (1 mentions)
Tga dsc 3, by Mettler Toledo (1 mentions)
Freestyle 293 f cells, by Thermo Fisher Scientific (1 mentions)
293fectin, by Thermo Fisher Scientific (1 mentions)
Opti mem 1 reduced serum medium, by Thermo Fisher Scientific (1 mentions)
Whatman no 1 filter paper, by Cytiva (1 mentions)
Bhi broth, by BD (1 mentions)
Pinaacle 900t, by PerkinElmer (1 mentions)
Sabouraud dextrose broth (sdb), by BD (1 mentions)
Bca protein kit, by Thermo Fisher Scientific (1 mentions)
Fv1200 confocal microscope, by Olympus (1 mentions)
Dimethyl sulfoxide (dmso), by Serva Electrophoresis (1 mentions)
Elisa plate reader, by Agilent Technologies (1 mentions)
44 protocols using orbital shaker
1
Aqueous Extraction of Moringa oleifera Leaves
2
Extraction of Bioactive Compounds from Agrocybe aegerita Mycelial Culture
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The extract preparation from the mycelial culture of Agrocybe aegerita was performed by a modified solvent evaporation method outlined by Shashikant et al. [14 (link)]. Briefly, the mushroom mycelial culture was cultured in an Erlenmeyer flask (250 mL) that contained malt extract broth. The sample was incubated at 27 °C for fifteen days. The mycelial culture was then filtered using Whatman filter paper 1 and dried at 32 °C for 72 h. The sample was ground to a fine powder using a mechanical grinder (Glen, 350 W, 20,000 RPM mixer, grinder, blender, Dublin, Ireland). The powdered sample (5 g) was then mixed with 50 mL of absolute ethanol and a conical flask containing the mixture was kept in shaking condition in an orbital shaker (Thermo Scientific, Mumbai, India) at 500 rpm for 48 h. The supernatant was filtered using Whatman grade 4 paper. The supernatant was then kept at 4–7 °C in a refrigerator to evaporate the solvent ethanol. The milky white viscous extract thus obtained was kept in vials and stored at −20 °C for further analysis.
3
Determination of ATP Levels in Cultures
4
Characterization of Adsorbent Materials
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The fresh and loaded adsorbent was pulverised and characterised by applying X-Ray (XRD), diffractometer (Brucker D8 advance), X-Ray Fluorescence (XRF), (Delta Professional), Scanning Electron Microscopy (SEM), (Carl Zeiss FEGSEM Gemini 500) respectively to find the mineralogical, elemental composition and morphology of the adsorbent. The ionic concentrations of adsorbates and filtrates were measured using calibrated Inductively Coupled Plasma-Optical Emission Spectrometry (iCAP 7000SERIES). Agitation of the adsorbent and solutions was achieved using an Orbital shaker (Thermo Scientific, supplied by Lamworld Technologies Pty Ltd). Thermogravimetric analysis (TGA) was used to evaluate the mass loss and thermal stability of the adsorbent using TGA/DSC3+,manufactured by Mettler Toledo,2018. The functional groups present in MGBW before and after adsorption of heavy metals were determined using a Fourier Transform Infrared Spectrometer (FTIR) (Vertex 70V model), manufactured and supplied by Bruker GmbH in the year 2018.
5
Adventitious Root Growth Kinetics
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Plantlets were regenerated according to the method
of Ahmad et al.,50 (link) and the seed-derived
roots were collected after 30 days of plantlet development. At the
initial stage, the roots were shifted to NAA (0.5 mg/L)-augmented
MS media for stock culture development. The flasks containing roots
in liquid media were placed on an orbital shaker (Thermo Scientific;
Germany) in the dark for 30 days. After 30 days, a known and uniform
amount of adventitious roots were exploited for each experiment and
each treatment. All experiments were carried out in 250 mL Erlenmeyer
flasks comprising 50 mL of MS media with different concentrations
of melatonin (0.5–5.0 mg/L), 30 g/L sucrose, and a pH level
of 5.6–5.8. Fresh adventitious root biomass and growth kinetics
were studied at 7 day intervals for a period of 56 days. The growth
curve was established for growing adventitious roots. The color and
morphology of adventitious roots were studied along with the growth
kinetics. The details of the experiment and their treatments are given
inTable 1 .
of Ahmad et al.,50 (link) and the seed-derived
roots were collected after 30 days of plantlet development. At the
initial stage, the roots were shifted to NAA (0.5 mg/L)-augmented
MS media for stock culture development. The flasks containing roots
in liquid media were placed on an orbital shaker (Thermo Scientific;
Germany) in the dark for 30 days. After 30 days, a known and uniform
amount of adventitious roots were exploited for each experiment and
each treatment. All experiments were carried out in 250 mL Erlenmeyer
flasks comprising 50 mL of MS media with different concentrations
of melatonin (0.5–5.0 mg/L), 30 g/L sucrose, and a pH level
of 5.6–5.8. Fresh adventitious root biomass and growth kinetics
were studied at 7 day intervals for a period of 56 days. The growth
curve was established for growing adventitious roots. The color and
morphology of adventitious roots were studied along with the growth
kinetics. The details of the experiment and their treatments are given
in
6
Culturing Cryptococcus neoformans H99
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
C. neoformans strain H99 (serotype A) was isolated and kindly provided by John Perfect at Duke University. Yeasts were grown in Sabouraud dextrose broth (pH 5.6; BD Difco) for 24 h at 30°C in an orbital shaker (Thermo Fisher) set at 150 rpm (to early stationary phase).
7
VEGF Encapsulation in PLGA-MNS Nanoformulation
8
Transient Antibody Production in 293F Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Recombinant mAbs were transiently produced in FreeStyle 293F cells (ThermoFisher Scientific) following the protocol detailed by Vink et al (Vink, Oudshoorn-Dickmann, Roza, Reitsma, & de Jong, 2014 (link)). First, the cells were suspended in FreeStyle 293 Expression Medium (ThermoFisher Scientific) at a concentration of 1.1×10^6 cells/ml, and 2 ml of the suspension were added to individual wells of a 24-well deep well plate. The cells were transfected with a 2 μg mixture of plasmids encoding the heavy chain (690 ng), light chain (690 ng), human p21 (Invivogen; 100 ng), human p27 (Invivogen; 500 ng), and SV40 large T antigen (20 ng) suspended in Opti-MEM I Reduced-Serum Medium (ThermoFisher Scientific), mixed with 293fectin (ThermoFisher Scientific), and added to the cells per the manufacturer’s instructions. The p21-, p27-, and SV40 large T antigen-encoding plasmids were included to enhance antibody production, as previously described (Vink et al., 2014 (link)). After transfecting the cells, the plate was clamped into a Microflask system (Applikon Biotechnology) affixed to an orbital shaker (Thermo Scientific) in a 37°C, 5% CO2 incubator. The supernatant was harvested after 7 days.
9
Extraction of Citrus nobilis Peel Polyphenols
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Citrus nobilis peels were kept for drying in a hot air oven at 30 °C to remove the water content. The dried peels were ground using a mechanical grinder (Sujata, mixer grinder, 900 watts) and the powder thus obtained was used for the preparation of the extract. Herein, the modified solvent evaporation technique was employed for extract preparation. Briefly, 40 g powdered peel was dispersed in 400 mL (1:10 w/v ratio) of absolute methanol in a conical flask and kept in an orbital shaker (Thermofisher Scientific Pvt. Ltd., Mumbai, India) for 72 h. To obtain the modified evaporated extract, the sample was filtered using Whatman filter paper No. 1 and evaporation of methanol was done at refrigerated temperature (4–7 °C) for 72 h. At refrigerated temperature, polyphenolic components were dispersed in methanol and molecules of methanol collected enough kinetic energy from its exchange with neighbor molecules to escape from the bonds with another molecule, hence molecules leave the mass of liquids and join the air as a vapor. The obtained dry extract was stored at −20 °C in airtight glass tubes for further use [8 (link)].
10
Magnesium-Loaded PLGA Nanoparticle Synthesis
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!