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Poly 1 c tlrl pic

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Poly(I:C) (tlrl-pic) is a synthetic double-stranded RNA (dsRNA) molecule that mimics the structure of viral RNA. It is commonly used as an immune stimulant and activator of the innate immune response.

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Lab products found in correlation

Mg132 s1748, by Beyotime (1 mentions) Z vad fmk c1202, by Beyotime (1 mentions) Tlrl chq, by InvivoGen (1 mentions) Bx795 tlrl bx7, by InvivoGen (1 mentions) Ht dna, by Merck Group (1 mentions) Starspin animal dna kit, by GenStar (1 mentions) Nc sirna, by GenePharma (1 mentions) Lipofectamine 2000, by Thermo Fisher Scientific (1 mentions) Lipofectamine rnaimax reagent, by Thermo Fisher Scientific (1 mentions) Lipofectamine rnaimax, by Thermo Fisher Scientific (1 mentions) Lps from escherichia coli o111 b4, by Merck Group (1 mentions) Recombinant human ifn γ, by BioLegend (1 mentions) Pierce high capacity endotoxin removal spin column, by Thermo Fisher Scientific (1 mentions) Dexamethasone (dex), by Merck Group (1 mentions) Mitotempo, by Merck Group (1 mentions) Il 13, by BioLegend (1 mentions) Methyl β cyclodextrin, by Merck Group (1 mentions) Tnf α, by BioLegend (1 mentions) Recombinant human il 10, by Thermo Fisher Scientific (1 mentions) Fsllry nh2, by Bio-Techne (1 mentions)

Spelling variants of this product

Poly(I:C) (721 citations) Tlrl-pic (56 citations) Poly(I:C) (PIC) (6 citations) High molecular weight poly(I:C) (tlrl-pic) (3 citations) POLY I (2 citations) Poly(I:C) HMW (tlrl-pic) (2 citations)

7 protocols using poly 1 c tlrl pic

1

Investigating the Role of HSP27 and OAS1 in Viral Infection

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The empty vectors pCMV-HA and pCMV-Myc were provided by the Biomedical Research Center of Northwest Minzu University. The recombinant plasmids Myc-HSP27, Myc-OAS1, Flag-MDA5, HA-VP1, HA-VP2, HA-2A, HA-2C, HA-3A, Myc-VP3, and Myc-3C were all constructed in the lab. The proteasome inhibitor MG132 (S1748) and caspase inhibitor Z-VAD-FMK (C1202) were obtained from Beyotime (Shanghai, China). The autolysosome inhibitor chloroquine (tlrl-chq) and poly (I:C) (tlrl-pic) were obtained from InvivoGen (San Diego, CA, United States). The small interfering RNA (siRNAs) an analog of double-stranded RNA (dsRNA) targeting homo HSP27 were designed and synthesized by Guangzhou Ribo Biotechnology (China). The siRNA sequences are listed in Table 1. A549 cells in 6-well plates were transfected with siRNAs or various plasmids using Lipofectamine™ 2000 according to the manufacturer’s instructions.
Li X., Ma R., Wu B., Niu Y., Li H., Li D., Xie J., Idris A, & Feng R. (2021). HSP27 Protein Dampens Encephalomyocarditis Virus Replication by Stabilizing Melanoma Differentiation-Associated Gene 5. Frontiers in Microbiology, 12, 788870.
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2

Poly(I-C) and 5'-ppp dsRNA Protocols

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Poly(I-C) (#tlrl-pic), and 5′-triphosphate double-strand RNA (5′-ppp dsRNA) (#tlrl-3prna) were purchased from InvivoGen.
Lin C.Y., Shih M.C., Chang H.C., Lin K.J., Chen L.F., Huang S.W., Yang M.L., Ma S.K., Shiau A.L., Wang J.R., Chen K.R, & Ling P. (2021). Influenza a virus NS1 resembles a TRAF3-interacting motif to target the RNA sensing-TRAF3-type I IFN axis and impair antiviral innate immunity. Journal of Biomedical Science, 28, 66.
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3

Cyclic GMP-AMP Signaling Pathway

Cited 1 time
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Cyclic [G(2′,5′)pA(3′,5′)p] (cGAMP) was obtained from BioLog. The dsDNA (HSV-1 60mer) was synthesize and annealed by DNA technology; sense strand 5′- CCA TCA GA AAG AG GTT TAA TA TTT TTG TGA GAC CAT CGA AGA GAG AAA GAG ATA AAA CTT; antisense 5′- AAG TTT TAT CTC TTT CTC TCT TCG ATG GTC TCA CAA AAA TAT TAA ACC TCT TTC TGA TGG. For more information see6 (link). The ssDNA1 sequences was 5′-GTC TCT CTG GTT AGA CCA GAT CTG AGC CTG GGA GCT CTC TGG CTA ACT AGG GAA CCC ACT GCT TAA GCC TCA ATA AAG CTT GCC TTG AGT GCT TCA AGT AGT G-3′. For more information see5 (link). Herring testis DNA was from Sigma Aldrich (D6898); BX795 (tlrl-bx7) and poly I:C (tlrl-pic) were both acquired from InvivoGen.
Jønsson K.L., Laustsen A., Krapp C., Skipper K.A., Thavachelvam K., Hotter D., Egedal J.H., Kjolby M., Mohammadi P., Prabakaran T., Sørensen L.K., Sun C., Jensen S.B., Holm C.K., Lebbink R.J., Johannsen M., Nyegaard M., Mikkelsen J.G., Kirchhoff F., Paludan S.R, & Jakobsen M.R. (2017). IFI16 is required for DNA sensing in human macrophages by promoting production and function of cGAMP. Nature Communications, 8, 14391.
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4

Investigating IRF3 Signaling Pathway

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Anti-p-IRF3 (ab76493) and anti-IRF3 (ab68481) were from Abcam; EGCG (E4143), RSVL (R5010) and HT-DNA (D6898) were from Sigma-Aldrich; Poly(I:C) (tlrl-pic) was from InvivoGen; Anti-G3BP1 (13057-2-AP) was from Proteintech Group; Plasmid DNA, used as the DNA stimulator, was an empty vector plasmid (pCDX-Tet-On) and amplified with PureYield Plasmid Midiprep System (A2492, Progema); Genomic DNAs were purified using StarSpin Animal DNA Kit (D111-01, GenStar); Anti-human cGAS and anti-human GAPDH antibodies were gifts from Dr. Tao Li at National Center of Biomedical Analysis, Beijing, China.
Li J., Zhang F., Bian W., Chen Y., Liu J., Liu Z., Xiong Y, & Wan X. (2022). cGAS inhibition alleviates Alu RNA-induced immune responses and cytotoxicity in retinal pigmented epithelium. Cell & Bioscience, 12, 116.
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5

REV-ERB Regulation of Innate Immunity

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pCMV-Tag2B was kindly provided by Professor Ying Xu (Cambridge Suda Genome Resource Center). A fragment of REV-ERBα or REV-ERBβ was inserted into pCMV-Tag2B. Plasmids were transfected into cells using Lipofectamine 2000 (Thermo Fisher Scientific) for 48 h. 50 nM negative control siRNA (NC siRNA) or REV-ERBα-specific siRNA (GenePharma) were transfected into cells using Lipofectamine RNAiMAX reagent (Thermo Fisher Scientific) for 48 h. Knockdown efficiency was evaluated using quantitative PCR. Poly (I:C) (tlrl-pic) and LTA (tlrl-pslta) were purchased from Invivogen and transfected into MCF7 cells or Caco-2 cells for 48 h. SR9009 (HY-16989) and GSK4112 (HY-14414) used in vitro were purchased from MCE.
Sun L., Huang K., Deng Q., Zhu Y., Cao Y., Dong K., Yang S., Li Y., Wu S, & Huang R. (2024). REV-ERBα negatively regulates NLRP6 transcription and reduces the severity of Salmonella infection in mice. Heliyon, 10(8), e28432.
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6

Interferon Signaling Pathway Modulation

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Reagents were obtained from Sigma-Aldrich (St. Louis, MO, USA) unless otherwise indicated. 3p-hpRNA (tlrl-hprna) and Poly(I:C) (tlrl-pic) were purchased from InvivoGen (San Diego, CA, USA). Recombinant human IFN-β (8499-IF-010/CF) and IL-29/IFNλ1 (1598-IL) were purchased from R&D Systems (Minneapolis, MN, USA). Recombinant human IFN-γ (570204) and the potent STAT1 inhibitor, S14-95 (ALX-350–299), were purchased from BioLegend (San Diego, CA, USA) and Enzo Life Sciences (Farmingdale, NY, USA), respectively. Lipofectamine RNAiMAX was purchased from Invitrogen (Carlsbad, CA, USA).
Shikama Y., Kurosawa M., Furukawa M., Kudo Y., Ishimaru N, & Matsushita K. (2022). The Priming Potential of Interferon Lambda-1 for Antiviral Defense in the Oral Mucosa. Inflammation, 45(3), 1348-1361.
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7

Endotoxin-Depleted House Dust Mite Extract Protocol

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House dust mite extract (# B84, Dermatophagoides pteronyssinus, Greer Laboratories, Inc., Lenoir, NC) was depleted of endotoxin using Pierce™ High Capacity Endotoxin Removal Spin Columns (# 88274, ThermoFisher Scientific, Waltham, MA) to generate a final concentration of 0.096 endotoxin units (EU) per microgram of HDM protein. Recombinant human IFN-γ(# 570204), TNF-α(# 570104), IL-13 (# 571104), IL-17A (# 570504), and M-CSF (# 574806) were from BioLegend (San Diego, CA). Poly (I:C) (# tlrl-pic) and polymyxin B (# tlrl-pmb) were from InvivoGen (San Diego, CA). E64 (# 5208), AEBSF (# 5175), protease activated receptor-1 (PAR-1) antagonist, RWJ 56110 (# 2614), and PAR-2 antagonist, FSLLRY-NH2 (# 4751), were from Tocris (Minneapolis, MN). Recombinant human APOE3 (# 350-02) and recombinant human IL-10 (# 200-10) were from PeproTech (Rocky Hill, NJ). The Recombinant human APOE3 contained 0.021 EU/μg of protein. Dexamethasone (# D4902), LPS from Escherichia coli O111:B4 (# L4391), MitoTEMPO (# SML0737), CA-074 methyl ester (# C5857), A-438079 hydrochloride hydrate (# A9736), E-64d (# E8640), potassium chloride (KCL) (# 60142), methyl-β-cyclodextrin (# C4555), α-cyclodextrin (# C4680) and cholesterol-loaded, water soluble, methyl-β-cyclodextrin (# C4951) were from Millipore Sigma (St. Louis MO). Z-YVAD-FMK (# 218746) and MCC950 (# 5.38120.0001) were from Calbiochem (Burlington, MA).
Gordon E.M., Yao X., Xu H., Karkowsky W., Kaler M., Kalchiem-Dekel O., Barochia A.V., Gao M., Keeran K.J., Jeffries K.R, & Levine S.J. (2019). Apolipoprotein E is a Concentration-dependent Pulmonary Danger Signal that Activates the NLRP3 Inflammasome and IL-1β Secretion by Asthmatic Macrophages. The Journal of allergy and clinical immunology, 144(2), 426-441.e3.
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