All experimental procedures conducted in this study adhered to the “Guidelines for the Care and Use of Laboratory Animals” published by the U.S. National Institutes of Health. Additionally, the Institutional Animal Care and Use Committee (IACUC) of National Taiwan University College of Medicine approved all experimental protocols (IACUC Approval No: 20190605). In order to exclude that the menstrual cycle of female rats may affect the experimental results, male rats were selected as experimental animals in this experiment. Male Wistar rats weighing between 270 and 320 g, approximately three months old, were obtained from BioLASCO Taiwan Co., Ltd., Taipei, Taiwan. The rats were housed in groups of three in Plexiglas cages and provided with ad libitum access to food and water. The animal housing room maintained a controlled temperature of 22 ± 3 °C and a 12/12 h light/dark cycle, with lights turned on at 7:00 a.m. To minimize animal anxiety and discomfort, a gentle handling procedure was implemented prior to the commencement of the experimental procedures. Each rat was handled for 20 min per day, for a duration of four days, in order to acclimate them to human interaction and reduce any potential stress.
Wistar rats
Manufactured by BioLASCO
Sourced in Taiwan, Province of China
Wistar rats are a commonly used laboratory rat strain. They are known for their docile temperament and are widely used in various research fields, including biomedical, pharmacological, and behavioral studies.
Automatically generated - may contain errors
Lab products found in correlation
Streptozotocin (stz), by Merck Group (2 mentions)
Antibiotic solution, by Thermo Fisher Scientific (2 mentions)
Lab diet 5001, by Land O'Lakes (1 mentions)
Elastase, by Merck Group (1 mentions)
Collagenase, by Merck Group (1 mentions)
Rodent 5001, by LabDiet (1 mentions)
C57bl 6 mice, by BioLASCO (1 mentions)
Mak076, by Merck Group (1 mentions)
Aβ1 42, by AnaSpec (1 mentions)
N2 supplement, by Thermo Fisher Scientific (1 mentions)
T75 culture flask, by Corning (1 mentions)
Dmem f12, by Thermo Fisher Scientific (1 mentions)
Neurobasal, by Thermo Fisher Scientific (1 mentions)
Ara c, by Merck Group (1 mentions)
L glutamine, by Merck Group (1 mentions)
Poly d lysine, by Merck Group (1 mentions)
Xylazine, by Merck Group (1 mentions)
Ketamine, by Pfizer (1 mentions)
Magnolol, by Merck Group (1 mentions)
Zoletil vet, by Virbac (1 mentions)
60 protocols using wistar rats
1
Handling Procedure for Male Wistar Rats
2
Wistar Rat Model of Type 1 Diabetes
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Twenty-four male Wistar rats (10 weeks-old, 200–250 g, BioLASCO Taipei, Taiwan ) were adapted in an animal room in a 12 h light–dark cycle with 25 °C of ambient temperature for 2 weeks. Rats were then divided into three groups including Sham (rats injected normal saline), T1DM (rats injected 50 mg/kg of streptozotocin via intraperitoneal route with fasting blood glucose level higher than 200 mg/dL) and T1DM+WJSC (T1DM rats receiving 1 × 106 WJSC/rat through intravenous route). All experimental rats were sacrificed after receiving WJSC for 2 months. The heart tissues were isolated and preserved at −80 °C in a freezer for further study. The protocol for animal study was approved by the Institute Animal Care and Use Committee of National Taiwan Normal University (Protocol number 109030).
3
Retinal Ischemia-Reperfusion Injury
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The following research has strictly obeyed the rules set by the ARVO Statement for the Use of Animals in Ophthalmology and Vision Research. In addition, the agreement to perform the research was received from the institutional review board of Cheng-Hsin General Hospital (Taipei, Taiwan; Approval No: CHIACUC 106-09). In terms of the animals, six-week-old Wistar rats (BioLasco, Taipei City, Taiwan) were ordered and raised at a humidity of 40–60% and a temperature of 19–23 °C. The rats were reared on a 12 h light/dark cycle together with 12~15 air refreshments per hour and were provided with food and water at liberty. Then, the animals were randomly allocated into normal, post-ischemic vehicle (I/R + Vehicle), and post-ischemic treatment groups (I/R + SAC).
4
Wistar Rat Anesthesia and Care
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For this study, 8-week-old male Wistar rats were procured from BioLASCO Taiwan (Taipei, Taiwan). The animal room was set to a photoperiod of 12-h light–dark cycle, with the humidity and temperature maintained at 50–55% and 24 ± 1 °C, respectively, using an air conditioning system throughout the experimental period. During the experiment, the rats weighed approximately 300 ± 10 g and were between 9 and 10 weeks old. Regardless of the experimental mode, all surgical procedures and feeding of the rats were completed after they were anesthetized with 2.5% isoflurane (catalog no. 08547, Panion & BF Biotech, Taoyuan, Taiwan). The handling and experimental use of animals conformed to the Guiding Principles for the Care and Use of Animals of the American Physiological Society, and the study protocol was approved by the Institutional Animal Care and Use Committee of National Sun Yat-sen University (approval number: 10725).
5
Aging Rats Model with WJSC Treatment
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Male 8-week-old Wistar rats (BioLASCO Taiwan Co., Ltd., Taipei, Taiwan) were randomly divided into three groups (n = 6), namely, Sham, Aging (D-galactose 200 mg/100 g BW per day, via intraperitoneal injection), and Aging + WJSC (with aging rats receiving 1 × 106 stem cells per rat via tail vein). All the experimental rats were adapted in the animal room with a 12 h light-dark cycle, a 25 °C ambient temperature, and standard chow (Lab Diet 5001, PMI Nutrition International Inc., Brentwood, MO, USA). Aging rats were injected with WJSC when they were found to have a heart disorder, as confirmed by a cardiac echo. After WJSC treatment for 8 weeks, all the rats were sacrificed, and the hearts were isolated for further studies. The animal study was approved by animal IRB (Institutional Animal Care and Use Committee of National Taiwan Normal University, protocol number 108021), and the authors of this study can confirm that the animal model followed the rules set out in the Declaration of Helsinki.
6
Isolation of Vascular Smooth Muscle Cells from Rat Aorta
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VSMCs were isolated from the thoracic aortas of male Wistar rats (150–200 g) by the method described previously [31 (link)]. Wistar rats were purchased from BioLASCO (Taipei, Taiwan). The study was conducted in accordance with the guidelines of the Animal Research Committee of National Taiwan University, College of Medicine, for the care and use of laboratory animals. Before experiments began, rats were allowed at least 1 week acclimation period at animal quarters with air conditioning and constant humidity. The light was controlled automatically at an interval of 12 h per day. The animals were allowed free access to food and water. To prepare VSMCs, the thoracic aortas were cleaned of fat and adventitia, cut into small strips, and then digested with 1 mg/mL collagenase (Sigma) and 0.125 mg/mL elastase (Sigma) at 37°C for 60 min. Cells were cultured in DMEM containing 10% FCS at 37°C in a humidified atmosphere of 5% CO2/95% air. Cells exhibited characteristics of VSMCs were used between the third and sixth passages.
7
Chitosan Oligosaccharide Attenuates Diabetes in Rats
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The animal experiments were approved by the Animal House Management Committee of the National Taiwan Ocean University and were conducted by the guidelines for care and use of laboratory animals. Five-week-old male Wistar rats were purchased from BioLASCO (Taipei, Taiwan). Animals were housed in cages in an animal room maintained at appropriate temperature and relative humidity and a 12 h light/12 h dark cycle. Animals could be free access to a standard rodent feed LabDiet Rodent 5001 (St. Louis, MO, USA) and water. Rats were divided into six groups: normal control (NC group), HF diet (HF group), STZ-induced diabetes with HF diet (DF group), DF with 0.5% COS (D0.5F group), DF with 1% COS (D1F group), and DF with 5% COS (D5F group). The dosages for COS were selected according to the previous reports [23 (link),28 (link)] and our preliminary tests. These diet compositions were shown in Table 4 . Rats received 50 mg/kg STZ in 0.1 M citrate by subcutaneous injection to induce diabetes. The experimental period was 4 weeks. Rats were weighed once a week and their food and water intake were weighed three times a week. Urine samples were collected during the last 3 days before animal euthanasia.
8
Wistar Rat Bioassay Protocol
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Thirty 8-week-old male Wistar rats (BioLasco Taiwan, Ilan, Taiwan) were used in this study. Rats were caged individually at 23 ± 2 °C and 55% ± 10% relative humidity with a 12 h dark/light cycle. Animal experiments were approved by the Institutional Animal Care and Use Committee of Taipei Medical University (LAC-2017-0384).
9
Wistar Rat Parkinson's Model
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Twenty male Wistar rats (BioLASCO Co. Ltd., Taipei, Taiwan) at eight weeks old, weighing 260–285 g, were used in the experiments. The 20 rats were randomly divided into four groups: control, 6-OHDA treatment, 6-OHDA plus 11-de, and 11-de alone group (five animals in each group). All studies were reported in accordance with the ARRIVE guidelines for experiments involving animals [41 (link),42 (link)]. The rats were maintained in Plexiglas cages in a temperature-controlled (22 °C) room, under a 12-h light/dark cycle, and given free access to food and water.
10
Wistar Rat Nutrition and Welfare
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A total of 96 male Wistar rats (body weight = 250 ± 30 g; Biolasco Taiwan Co., Taipei, Taiwan) were used. The rats were kept on a 12-h light–dark cycle and had a free access to water and food. All experimental procedures were approved by the Institutional Animal Care and Use Committee of National Taiwan University and were conducted in accordance with its guidelines.
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