Frozen supernatants from the long-term co-culture assay, collected at day 3, day 6, and day 13 (from 22Rv1 and 22 Rv1-KO target cells co-cultures), and at day 18 (from C4-2B shGFP and C4-2B shSTEAP1 target cells co-cultures), were thawed, and the secreted cytokines and chemokines were quantified using the Bio-Plex Pro Human Immunotherapy panel, 20-plex (Bio-Rad, Oslo, Norway, 12007975) according to the manufacturer’s instructions. Samples were measured on a Luminex 200 instrument system (Bio-Rad, Oslo, Norway) and evaluated using the Bio-Plex Manager™ software, version 6.2. Supernatants from each donor were analyzed in duplicate. Each duplicate pair was kept separate from T cell stimulation until the end of the experiment.
Luminex 200 instrument system
Manufactured by Bio-Rad
Sourced in Norway
The Luminex 200 instrument system is a multiplex analysis platform that utilizes flow cytometry technology to detect and quantify multiple analytes simultaneously in a single sample. The system is capable of analyzing up to 100 different targets in a single well or tube. It is designed for applications that require high-throughput and multiplexed assays, such as protein expression profiling, cytokine/chemokine analysis, and infectious disease detection.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using luminex 200 instrument system
1
Quantifying Cytokine Profiles in Co-Culture
2
Cytokine Profiling in Rats
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Interleukin 1 α (IL-1α), Interleukin 1β (IL-1β), Interleukin 2 (IL-2), Interleukin 6 (IL-6), Interleukin 12(IL-12), Interferon γ (IFN-γ), and Tumor necrosis Factor α (TNF-α) were measured by a rat cytokine magnetic bead panel (Bio-Rad Laboratories, Hercules, CA) and measured by a Luminex™ 200™ Instrument System and Bio-Plex Manager Software (Bio-Rad Laboratories, Hercules, CA) according to manufacturer’s instruction.
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