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Triacylglycerol tg

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Triacylglycerol (TG) is a type of lab equipment used to measure the concentration of triglycerides, a type of fat, in biological samples. It functions by providing a quantitative analysis of triglyceride levels.

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Lab products found in correlation

Phosphatidylethanolamine, by Avanti Polar Lipids (3 mentions) Phosphatidylcholine, by Avanti Polar Lipids (2 mentions) Free fatty acid, by Merck Group (2 mentions) Phosphatidylinositol, by Avanti Polar Lipids (2 mentions) Lysophosphatidylethanolamine lpe, by Avanti Polar Lipids (2 mentions) Lysophosphatidylinositol, by Avanti Polar Lipids (2 mentions) Arachidonic acid, by Merck Group (1 mentions) Phosphatidylserine, by Avanti Polar Lipids (1 mentions) Phosphatidic acid, by Avanti Polar Lipids (1 mentions) Phosphatidylglycerol, by Avanti Polar Lipids (1 mentions) Lysophosphatidylethanolamine, by Avanti Polar Lipids (1 mentions) Kmc006, by Thermo Fisher Scientific (1 mentions) Phosphatidylcholine pc, by Avanti Polar Lipids (1 mentions) Lysophosphatidylcholine lpc, by Avanti Polar Lipids (1 mentions) Lysophosphatidylcholine, by Avanti Polar Lipids (1 mentions) Milli q system, by Merck Group (1 mentions) Acetonitrile, by Merck Group (1 mentions) Hplc grade methanol, by Merck Group (1 mentions)

5 protocols using triacylglycerol tg

1

Lipid Standards for Analytical Chemistry

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Lipid standards including phosphatidylcholine (PC) 16:0/18:1 (9Z), PC 18:1 (9Z)/16:0, PC 18:1 (9Z)/18:1 (9Z), PC 18:1 (6Z)/18:1 (6Z), phosphatidylethanolamine (PE) 18:1 (9Z)/18:1 (9Z), lysophosphatidylethanolamine (LPE) 18:1 (9Z), phosphatidylglycerol (PG) 18:0/18:1 (9Z), phosphatidic acid (PA) 16:0/18:1 (9Z), phosphatidylserine (PS) 16:0/18:1 (9Z) and ceramide (Cer) d18:1/16:0 were purchased from Avanti Polar Lipids (AL, USA). Fatty acids including FA 18:1 (9Z), FA 18:1 (9E), FA 18:1 (6Z), FA 18:1 (11Z), FA 20:1 (11Z), FA 20:1 (11E), FA 18:2 (9Z, 12Z) and arachidonic acid FA 18:4 (5Z, 8Z, 11Z, 14Z), and triacylglycerol (TG) 18:1 (9Z)/18:1 (9Z)/18:1 (9Z) were purchased from Sigma-Aldrich. Other solvents were purchased from Innochem (Beijing, China) and meet or exceed the analytical grade standard. The structures of all pure lipids are shown in Fig. S1 and S2.† Anthraquinone was purchased from Rhawn (Shanghai, China).
Feng G., Hao Y., Wu L, & Chen S. (2020). A visible-light activated [2 + 2] cycloaddition reaction enables pinpointing carbon–carbon double bonds in lipids. Chemical Science, 11(27), 7244-7251.
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2

Blood Metabolites in Aging Hens

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Overnight fasting blood samples were collected from 6 randomly selected hens in each treatment group, consisting of hens at 58 and 65 weeks of age from a wing vein. On those same days, a second blood sample was collected 30 min after re-feeding 20 g of feed. Commercial kits were used to determine plasma triacylglycerol (TG, Sigma-Aldrich, St. Louis, MO, USA), glucose and NEFA (non-esterified fatty acid, Wako Chemicals, Osaka, Japan) levels. Plasma insulin and interleukin-1β (IL-1β) concentrations were determined by a validated commercial ELISA kit (Cat.# 10-1249-01, Uppsala, Sweden, KMC006; Biosource International, Camarillo, CA, USA, respectively) [28 (link),29 (link)].
Chou P.C., Lin P.C., Wu S.W., Wang C.K., Chung T.K., Walzem R.L., Lai L.S, & Chen S.E. (2021). Differential Modulation of 25-hydroxycholecalciferol on Innate Immunity of Broiler Breeder Hens. Animals : an Open Access Journal from MDPI, 11(6), 1742.
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3

Authentic Internal Standards for Lipidomic Analysis

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The authentic internal standards (IS) for lipidomic analysis, namely, phosphatidylcholine (PC) 13:0/13:0, phosphatidylethanolamine (PE) 15:0/15:0, phosphatidylinositol (PI) 8:0/8:0, lysophosphatidylcholine (LPC) 15:0, lysophosphatidylethanolamine (LPE) 13:0, and lysophosphatidylinositol (LPI) 13:0, were purchased from Avanti Polar Lipids (Alabaster, AL), while triacylglycerol (TG) 11:0/11:0/11:0 and free fatty acid (FFA) 17:0 were obtained from Sigma-Aldrich (St. Louis, MO). Unless specified, other chemicals and reagents were of the highest grade available and purchased from Sigma-Aldrich.
Chen Z., Sai S., Nagumo K., Wu Y., Chiba H, & Hui S.P. (2023). Distinctive serum lipidomic profile of IVIG-resistant Kawasaki disease children before and after treatment. PLOS ONE, 18(3), e0283710.
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4

Lipid Profiling Internal Standards

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For the authentic internal standards (IS), triacylglycerol (TG) 11:0/11:0/11:0 and free fatty acid (FFA) 17:0 were obtained from Sigma-Aldrich (St. Louis, MO, USA), and phosphatidylcholine (PC) 13:0/13:0, phosphatidylethanolamine (PE) 15:0/15:0, phosphatidylinositol (PI) 8:0/8:0, lysophosphatidylcholine (LPC) 15:0, lysophosphatidylethanolamine (LPE) 13:0, and lysophosphatidylinositol (LPI) 13:0 were purchased from Avanti Polar Lipids (Alabaster, AL, USA). Cardiolipin (CL) 15:0/15:0/15:0/15:0 was synthesized in our laboratory previously [16 (link)]. Other chemicals and reagents, which were of the highest grade available, were purchased from Sigma-Aldrich unless specified. The mixed solution of all the IS was prepared with methanol (containing 0.006% butylated hydroxytoluene, w/v) and stored at −80 °C for no more than two weeks before use.
Wu Y., Chen Z., Fuda H., Tsukui T., Wu X., Shen N., Saito N., Chiba H, & Hui S.P. (2021). Oxidative Stress Linked Organ Lipid Hydroperoxidation and Dysregulation in Mouse Model of Nonalcoholic Steatohepatitis: Revealed by Lipidomic Profiling of Liver and Kidney. Antioxidants, 10(10), 1602.
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5

Lipidomics Standards Initiative Protocol

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All method details and many other information are also provided in the attached reporting checklist of the lipidomics standards initiative (LSI) in the Supplemental Data. HPLC-grade methanol, acetonitrile (ACN), and isopropanol (IPA) were purchased from Merck (Darmstadt, Germany), HPLC-grade tert-butyl methyl ether (MTBE) and ammonium acetate from Sigma−Aldrich (St. Louis), HPLC-grade chloroform (CHCl3) from Duksan (Ansan-si, South Korea), and ultrapure water was prepared by a Milli-Q system (Millipore). All internal standards were purchased from Avanti Polar Lipids (Alabaster), except FA 22:0-d4 (ten Brink, Amsterdam, The Netherlands) and triacylglycerol (TG) 15:0/15:0/15:0 (Sigma-Aldrich).
Wang Q., Hoene M., Hu C., Fritsche L., Ahrends R., Liebisch G., Ekroos K., Fritsche A., Birkenfeld A.L., Liu X., Zhao X., Li Q., Su B., Peter A., Xu G, & Lehmann R. (2023). Ex vivo instability of lipids in whole blood: preanalytical recommendations for clinical lipidomics studies. Journal of Lipid Research, 64(6), 100378.
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