MSCs (2.4×103 cells/mm2) were cultured onto the basal surface (lower chamber) of 0.4 or 3 µm pore 6-well Transwell inserts for 24 h as described previously (Munir et al., 2016 (link), 2017 (link); McGettrick et al., 2017 (link)). The culture medium was removed from both chambers. Phosphate-buffered saline with Ca2+ and Mg2+ (PBS; Sigma) was added to the lower chamber. CD41a-labelled whole blood was added to the apical surface (upper chamber) of the filter for 1 h, before non-adherent cells were removed by washing. Adherent platelets were imaged using an Olympus X71 Fluorescent Invert microscope enclosed at 37°C. Adhesion and platelet aggregation were quantified by using the Particle Analysis of Fluorescence function in ImageJ (NIH) and expressed as platelet coverage in µm2.
Ca2 and mg2 pbs
Manufactured by Merck Group
Sourced in United States
Ca2+ and Mg2+ (PBS) is a laboratory product that provides a phosphate-buffered saline (PBS) solution containing calcium and magnesium ions. This solution is commonly used as a buffer and rinsing agent in various cell culture and biochemical applications.
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Lab products found in correlation
2 protocols using ca2 and mg2 pbs
1
Platelet Adhesion on MSC Monolayers
2
Oviduct Collection and Evaluation
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Paired oviducts along with their ipsilateral ovaries were collected from a local slaughterhouse, and they were closed from
both ends to prevent leakage or contamination of the oviduct contents. The stages of the estrous cycle were determined
macroscopically by assessing ovarian morphology through the observation of color, size, and weight of the corpus luteum, as
described previously [11 (link)]. Furthermore, oviducts as well as the attached uteri were
macroscopically examined to ensure that they were healthy and free from inflammation. Following these examinations, oviducts
were immersed in PBS without Ca2+ and Mg2+ (PBS–/–) (Sigma-Aldrich, St. Louis, MO, USA) and
supplemented with 0.3% amphotericin B (Sigma-Aldrich), and 0.3% gentamicin (Sigma-Aldrich).
both ends to prevent leakage or contamination of the oviduct contents. The stages of the estrous cycle were determined
macroscopically by assessing ovarian morphology through the observation of color, size, and weight of the corpus luteum, as
described previously [11 (link)]. Furthermore, oviducts as well as the attached uteri were
macroscopically examined to ensure that they were healthy and free from inflammation. Following these examinations, oviducts
were immersed in PBS without Ca2+ and Mg2+ (PBS–/–) (Sigma-Aldrich, St. Louis, MO, USA) and
supplemented with 0.3% amphotericin B (Sigma-Aldrich), and 0.3% gentamicin (Sigma-Aldrich).
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