Human rhabdomyosarcoma (RD; CCL-136; ATCC) cells were maintained in Dulbecco’s modified Eagle Medium (DMEM) containing 10% fetal bovine serum (FBS; Life Technologies) at 37°C in a 5% CO2 incubator. Echovirus 11 strain (NCBI Accession No. OP764694) was isolated from a feces sample of a 24-day-old female neonate with enterovirus infection after passaging in the RD cells. RD cells were infected with echovirus at various multiplicity of infection (MOI) for 1.5 h in serum-free DMEM. The cells were washed with phosphate-buffered saline (PBS) and cultured in a completely fresh medium for various times as indicated until they were harvested. For autophagy induction experiments, cells were infected or mock-infected with echovirus for 1.5 h, then cultured in a complete medium containing rapamycin (Selleck, AY-22989) at indicated concentrations for the indicated times. For autophagy inhibition experiments, cells were cultured in DMEM containing indicated concentrations of 3-methyladenine (3-MA) (Selleck, S2767) for 2 h, followed by echovirus infection for 1.5 h, and then incubated with fresh DMEM for 16 h. Cell counting kit-8 (CCK8) (Vazyme, A311-01) assay was performed to examine the cytotoxicity of rapamycin or 3-MA to RD cells.
Ccl 136
Manufactured by American Type Culture Collection
Sourced in United States, Germany
CCL-136 is a cell line derived from human embryonic kidney cells. It is a widely used tool in cell biology research and drug development. The core function of CCL-136 is to provide a standardized, renewable source of human kidney cells for in vitro experiments and assays.
Automatically generated - may contain errors
Lab products found in correlation
Dulbecco modified eagle medium (dmem), by Thermo Fisher Scientific (7 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (4 mentions)
Htb 37, by American Type Culture Collection (3 mentions)
Ccl 81, by American Type Culture Collection (3 mentions)
Crl 2061, by American Type Culture Collection (3 mentions)
Glutamax, by Thermo Fisher Scientific (3 mentions)
Htb 55, by American Type Culture Collection (2 mentions)
Ccl 171, by American Type Culture Collection (2 mentions)
293t ace2, by BEI Resources (2 mentions)
Hcov oc43, by BEI Resources (2 mentions)
Ht1080, by American Type Culture Collection (2 mentions)
Ccl 185, by American Type Culture Collection (2 mentions)
Minimum essential medium (mem), by Thermo Fisher Scientific (2 mentions)
L glutamine, by Thermo Fisher Scientific (2 mentions)
Mycoalert mycoplasma detection kit, by Lonza (2 mentions)
Ay 22989, by Selleck Chemicals (1 mentions)
S2767, by Selleck Chemicals (1 mentions)
A311 01, by Vazyme (1 mentions)
Us mo 14 18949, by American Type Culture Collection (1 mentions)
Us il 14 18952, by American Type Culture Collection (1 mentions)
32 protocols using ccl 136
1
Echovirus 11 Infection in RD Cells
2
Culturing and Amplification of Enteroviruses
3
Diverse Cell Lines Infected with EV-A71
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Human rhabdomyosarcoma (RD; ATCC #CCL-136), neuroblastoma (SH-SY5Y; ATCC #CRL-2266), African green monkey kidney cells (Vero; ATCC # CCL-81) and embryonal carcinoma cells (NTERA-2; ATCC # HTB-106) cells were maintained in DMEM (Invitrogen, USA) that was supplemented with 10% FBS (Gibco, Calif., USA), 1% L-glutamine, 1% non-essential amino acids and 1% penicillin/streptomycin. A neuro-virulent EV-A71 strain 5865/SIN/00009 (Accession No.: AF316321; sub-genotype B4; designated as Strain 41) was isolated from a deceased child during the Singapore HFMD outbreak in October 2000.
4
Evaluating Cell Lines for SARS-CoV-2 Research
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Human rhabdomyosarcoma cell line (RD, ATCC® CCL‐136™), African green monkey kidney cell line Vero C1008 (ATCC® CRL‐1586™), human hepatoma cell line Huh‐7 (a kind gift from Dr. Tianyi Wang at the University of Pittsburgh), and HEK293T expressing angiotensin‐converting enzyme 2 (ACE2) (293T‐ACE2, BEI Resources, NR‐52511) cell lines were maintained in Dulbecco's modified Eagle's medium (DMEM); human fibroblast cell line MRC‐5 (ATCC® CCL‐171™), human lung adenocarcinoma cell line Calu‐3 (ATCC® HTB‐55™), and human colorectal adenocarcinoma cell line (Caco‐2, ATCC® HTB‐37™) were maintained in Eagle's minimum essential medium (ATCC® 30‐2003™). Both media were supplemented with 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin antibiotics. Cells were kept at a cell culture incubator (humidified, 5% CO2/95% air, 37°C). The following reagents were obtained through BEI Resources, NIAID, NIH: HCoV, HCoV‐OC43, NR‐52725; HCoVs, HCoV‐NL63, NR‐470. HCoV‐OC43 was propagated in RD cell line; HCoV‐NL63 was initially propagated in 293T‐ACE2 cell line and accommodated in Vero E6 cell line. HCoV‐229E was obtained from Dr. Bart Tarbet (Utah State University) and amplified in Huh‐7 or MRC‐5 cell lines.
5
Cell Culture Protocols for Cancer Research
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Huh7 hepatocellular carcinoma cell line was sourced from JCRB cell bank (JCRB0403, Japan). Huh7, RD (CCL-136, ATCC, VA, USA) and NMuMG (CRL-1636, ATCC) cell lines were maintained in high glucose Dulbecco’s Modified Eagle Medium (DMEM, Thermo Fisher Scientific, Waltham, MA, USA) containing 10% fetal bovine serum (FBS, Hyclone, Logan, UT, USA), 2 mM l -glutamine and antibiotics. HT-1080 (CCL-121, ATCC) cell line was maintained in Eagle’s Minimum Essential Medium (MEM, Thermo Fisher Scientific) containing 10% FBS and supplemented with 2 mM l -glutamine, 1 mM sodium pyruvate, and antibiotics.
6
Cell Culture Maintenance and Differentiation
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Human epithelial lung cell line A549 (ATCC® CCL-185™) and human rhabdomyosarcoma cell line RD (ATCC® CCL-136™) were maintained in MEM. Mediums were supplemented with 10% FBS, 100 U/mL penicillin, 100 μg/mL streptomycin and 2 mM L-glutamine. The human monocyte cell line THP-1 (ATCC TIB- 202™) were maintained in RMPI medium supplemented with 100 U/mL penicillin, 100 μg/mL streptomycin and 2 mM L-glutamine, 0.05 mM 2-mercaptoethanol and 1 mM sodium pyruvate. THP-1, monocytes, were differentiated into macrophages by treatment with 100 ng/mL PMA for 24 h. All cell lines were grown in a humidified atmosphere of 5% CO2 at 37 °C.
7
Rhabdomyosarcoma Cell Culture for EV-A71
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Rhabdomyosarcoma (RD, CCL-136, ATCC, Manassas, VA, USA) cells were maintained with Dulbecco’s Modified Eagle’s Medium (DMEM) supplemented with 10% fetal bovine serum (FBS) and 1% penicillin–streptomycin antibiotics (PSA). Enterovirus A71 (EV-A71) strain 41 (5865/SIN/000009 strain, Genebank accession no. AF316321.2) was propagated in RD cells.
8
Cell Culture Protocols for Cancer Cell Lines
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The human melanoma cells lines 501mel, MM200, and WM293a (kindly donated by Professor Dorothy Bennet, St George's University of London) were cultured in Roswell Park Memorial Institute Medium (RPMI)-1640 (Sigma Aldrich, USA). The human breast adenocarcinoma cell lines MCF-7 (ATCC® HTB-22) and T47D (ATCC® HTB-133) were cultured in Dulbecco's Modified Eagle medium:nutrient mixture F-12 (Ham) (DMEM/F-12) (Gibco by Thermo Fisher Scientific, USA). The human embryonal rhabdomyosarcoma cell line RD (ATCC® CCL-136) and the human alveolar rhabdomyosarcoma cell line RH30 (kindly provided by Professor Judith Davie, Southern Illinois University) were cultured in DMEM (Sigma Aldrich, USA). All culture medium was supplemented with 10% heat-inactivated fetal bovine serum (FBS), 100 U/mL penicillin and 100 μg/mL streptomycin. Cells were maintained at 37°C in a 5% CO2 humidified incubator and medium was replaced every 2–3 days.
9
Induction and Inhibition of Procollagen Transport
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Cells were grown in DMEM supplemented with 10% fetal bovine serum. RD human rhabdomyosarcoma cell line (ATCC; CCL-136) and the HT-1080 human osteosarcoma cell line (ATCC; CCL-121) were provided by Klaus Kuhn (Max Planck Institute, Germany). COS-7 and HeLa cells were purchased from JCRB (Osaka, Japan; JCRB9127 COS-7) and ATCC (CRM-CCL-2), respectively. All cells were confirmed free from mycoplasma infection. TransIT LT1 (Takara, Otsu, Japan) and RNAi-MAX (Invitrogen) were used for the transfection of plasmids and siRNA, respectively. To induce ER-to-Golgi transport of procollagen III and GFP-COL3A1, ascorbic acid phosphate (Wako, Osaka, Japan) was added to the medium at a final concentration of 136 µg/ml. Cycloheximide (Nacalai Tesque, Kyoto, Japan) was dissolved in phosphate-buffered saline and added to the medium at a final concentration of 100 µM. To inhibit the ER-to-Golgi transport by temperature shift, culture medium was replaced with MEM with Hanks’s balanced salt solution (Thermo Fisher Scientific) precooled at 15°C, and the cells were incubated in a normal incubator without CO2 supply. To restart ER-to-Golgi traffic, the medium was replaced with DMEM prewarmed at 37°C and returned to a CO2 incubator set at 37°C.
10
Diverse Cell Lines for Coronavirus Propagation
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