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Anti phospho β catenin thr41 ser45

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Anti-phospho-β-catenin (Thr41/Ser45) is a primary antibody that specifically recognizes the phosphorylated form of β-catenin at threonine 41 and serine 45 residues.

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Lab products found in correlation

Anti phospho β catenin ser33 37 thr41, by Cell Signaling Technology (2 mentions) Dynabeads protein g, by Thermo Fisher Scientific (2 mentions) Luminol reagent, by Santa Cruz Biotechnology (2 mentions) Anti β catenin, by BD (1 mentions) Anti axin1, by Cell Signaling Technology (1 mentions) Anti axin2, by Cell Signaling Technology (1 mentions) Anti c ebpβ, by Santa Cruz Biotechnology (1 mentions) Horseradish peroxidase conjugated anti mouse igg, by Santa Cruz Biotechnology (1 mentions) Anti actin, by Merck Group (1 mentions) Anti c myc, by Santa Cruz Biotechnology (1 mentions) Gradient gel, by Thermo Fisher Scientific (1 mentions) Ecl system, by Santa Cruz Biotechnology (1 mentions) Anti cyclin d1, by Santa Cruz Biotechnology (1 mentions) Anti gfp, by Thermo Fisher Scientific (1 mentions) Nitrocellulose membrane, by Bio-Rad (1 mentions) Anti phospho β catenin, by Cell Signaling Technology (1 mentions)

3 protocols using anti phospho β catenin thr41 ser45

1

Western Blot Analysis of Wnt Signaling

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Cytosolic fractions were prepared as previously described30 (link). Proteins were separated by SDS-PAGE in a 4–12% gradient gel (Invitrogen, Carlsbad, CA, USA) and transferred to a nitrocellulose membrane (Bio-Rad, Hercules, CA, USA). The membrane was blocked with 5% nonfat milk and probed with anti-β-catenin (BD Transduction Laboratories, 610154, 1:1000), anti-phospho-β-catenin (Ser33/37/Thr41) (Cell Signaling Technology, #9561S, 1:1000), anti-phospho-β-catenin (Thr41/Ser45) (Cell Signaling Technology, #9565S, 1:1000), anti-Axin1 (Cell Signaling Technology, #2087S), anti-Axin2 (Cell Signaling Technology, #2151S, 1:1000), anti-C/EBPβ (Santa Cruz Biotechnology, sc-150, 1:500), anti-cyclinD1 (Santa Cruz Biotechnology, sc-20044, 1:500), anti-c-myc (Santa Cruz Biotechnology, sc-40, 1:500), anti-GFP (Invitrogen, A11122, 1:1000), and anti-actin (Sigma-Aldrich, A1978, 1:2000) antibodies. The membranes were then incubated with horseradish-peroxidase-conjugated anti-mouse IgG (Santa Cruz Biotechnology, sc-2004, 1:1000) or anti-rabbit IgG (Santa Cruz Biotechnology, sc-2031, 1:1000) and visualized using the ECL system (Santa Cruz Biotechnology, sc-2048).
Park S., Lee M.S., Gwak J., Choi T.I., Lee Y., Ju B.G., Kim C.H, & Oh S. (2018). CCAAT/enhancer-binding protein-β functions as a negative regulator of Wnt/β-catenin signaling through activation of AXIN1 gene expression. Cell Death & Disease, 9(10), 1023.
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2

Immunoprecipitation and Western Blot Analysis

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Cell lysates (50 μg) were incubated overnight at 4 °C with either anti-β-catenin or GSK3β at a 1:100 dilution. Dynabeads Protein G (Life Technologies) were added to the suspension for 2 h at 4 °C and the specific protein immunoprecipitated. After denaturing, the proteins were electrophoresed, transferred, and blocked. Primary antibodies (anti-phospho-GSK3β (Ser9), Cell Signaling no. 9323, anti-phospho-β-catenin (Thr41/Ser45), Cell Signaling no. 9565, and anti-phospho-β-catenin (Ser33/37/Thr41, Cell Signaling no. 9561)) were diluted 1:1000 in TBST and incubated with the blot overnight at 4 °C. The anti-rabbit HRP secondary antibody (1:2000) was incubated with the blot and proteins were detected using Luminol reagent (Santa Cruz). Band intensity was determined by densitometry, with MHC used as the loading control.
Bain L.J., Liu J.T, & League R.E. (2016). Arsenic inhibits stem cell differentiation by altering the interplay between the Wnt3a and Notch signaling pathways. Toxicology Reports, 3, 405-413.
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3

Immunoprecipitation and Western Blot Analysis of Phosphorylated GSK3β and β-Catenin

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Cell lysates (50μg) were incubated overnight at 4°C with either anti-β-catenin or GSK3β at a 1:100 dilution. Dynabeads Protein G (Life Technologies) were added to the suspension for 2 hours at 4°C and the specific protein immunoprecipitated. After denaturing, the proteins were electrophoresed, transferred, and blocked. Primary antibodies (anti-phospho-GSK3β (Ser9), Cell Signaling no. 9323, anti-phospho-β-catenin (Thr41/Ser45), Cell Signaling no. 9565, and anti-phospho-β-catenin (Ser33/37/Thr41, Cell Signaling no. 9561) were diluted 1:1000 in TBST and incubated with the blot overnight at 4°C. The anti-rabbit HRP secondary antibody (1:2000) was incubated with the blot and proteins were detected using Luminol reagent (Santa Cruz). Band intensity was determined by densitometry, with MHC used as the loading control.
Bain L.J., Liu J.T, & League R.E. (2016). Arsenic inhibits stem cell differentiation by altering the interplay between the Wnt3a and Notch signaling pathways. Toxicology Reports, 3, 405-413.
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