Ac220

Manufactured by Selleck Chemicals

Sourced in United Kingdom

AC220 is a laboratory equipment that provides alternating current (AC) power at 220 volts. It is designed for use in research and industrial settings where a stable and reliable AC power source is required.

Automatically generated - may contain errors

Lab products found in correlation

Dimethyl sulfoxide (dmso), by Merck Group (3 mentions) Sorafenib, by Selleck Chemicals (3 mentions) Pkc412, by Selleck Chemicals (3 mentions) Vosaroxin, by Selleck Chemicals (2 mentions) Etoposide, by Bio-Techne (2 mentions) 10058 f4 c myc inhibitor, by Merck Group (1 mentions) Fingolimod, by Cayman Chemical (1 mentions) Tc g 24, by Bio-Techne (1 mentions) Azd1208, by Bio-Techne (1 mentions) Mk 2206, by Selleck Chemicals (1 mentions) Annexin 5 apoptosis detection kit, by BD (1 mentions) Attune nxt flow cytometer, by Thermo Fisher Scientific (1 mentions) 96 well round bottom plate, by Corning (1 mentions) Click it plus edu flow cytometry assay kit, by Thermo Fisher Scientific (1 mentions) Cytarabine, by Pfizer (1 mentions) Mylotarg, by Pfizer (1 mentions) U 2os cell line, by American Type Culture Collection (1 mentions) Hek293t, by Leibniz Institute DSMZ (1 mentions) Recombinant murine il 3, by Immunotools (1 mentions) Ba f3, by Leibniz Institute DSMZ (1 mentions)

Spelling variants of this product

Quizartinib (AC220) (8 citations)

18 protocols using ac220

In vivo and in vitro drug preparation

Cited 2 times

Check if the same lab product or an alternative is used in the 5 most similar protocols

BEN (SelleckChem) was reconstituted and diluted for in vivo administration as previously described (24 (link)–27 (link)). AC220 (SelleckChem) and JSI-124 (Santa Cruz Biotechnology) were reconstituted in DMSO (Sigma-Aldrich). For in vitro studies, stock solutions of drugs were diluted in complete media (CM) consisting of RPMI-1640 with 10% FBS, 1% Sodium Pyruvate, 1% MEM NEAA, and 100 U/mL penicillin-streptomycin to their final concentrations.

Molina M.S., Hoffman E.A., Stokes J., Kummet N., Smith K.A., Baker F., Zúñiga T.M., Simpson R.J, & Katsanis E. (2021). Regulatory Dendritic Cells Induced by Bendamustine Are Associated With Enhanced Flt3 Expression and Alloreactive T-Cell Death. Frontiers in Immunology, 12, 699128.

+ Open protocol

+ Expand

Doxorubicin and AC220 Cytotoxicity

Check if the same lab product or an alternative is used in the 5 most similar protocols

We plated 1,000 cells/well in opaque, 96-well, flat-bottom plates (PerkinElmer), then treated them with or without 2 μg/mL dox (Sigma) and AC220 (Selleckchem) for 72 hours. Cell viability was analyzed with CTG (Promega).

Chen P.Y., Huang B.J., Harris M., Boone C., Wang W., Carias H., Mesiona B., Mavrici D., Kohler A.C., Bollag G., Zhang C., Zhang Y, & Shannon K. (2023). Structural and functional analyses of a germline KRAS T50I mutation provide insights into Raf activation. JCI Insight, 8(17), e168445.

+ Open protocol

+ Expand

Inhibitors of FLT3, PP2A, and Signaling Pathways in AML

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Gilteritinib (4 (link)) (ASP2215; Active Biochem, Maplewood, NJ) and quizartinib (4 (link)) (AC220; Selleck Chemicals, Houston, TX), type I and II FLT3 inhibitors, respectively, clinically active in FLT3-ITD AML, were used at pharmacologically relevant concentrations (23 (link),24 (link)). The SET-sequestering PAD FTY720 (25 (link)) (Fingolimod; Cayman Chemical Company, Ann Arbor, MI) was also used at pharmacologically relevant concentrations. DT-061, an orally bioavailable small molecule activator of PP2A (SMAP) developed by reengineering tricyclic neuroleptics and proposed to directly bind the PP2A Aα subunit (26 (link)–29 (link)), was provided by Dr. Goutham Narla. The pan-Pim inhibitor AZD1208 and GSK-3β inhibitors TC-G 24 and TWS119 were from Tocris Bioscience, Minneapolis, MN, the c-Myc inhibitor 10058-F4 from Sigma-Aldrich, and the pan-AKT inhibitor MK-2206 from Selleck Chemicals, Houston, TX.

Scarpa M., Singh P., Bailey C.M., Lee J.K., Kapoor S., Lapidus R.G., Niyongere S., Sangodkar J., Wang Y., Perrotti D., Narla G, & Baer M.R. (2021). PP2A-activating drugs enhance FLT3 inhibitor efficacy through AKT inhibition-dependent GSK-3β-mediated c-Myc and Pim-1 proteasomal degradation. Molecular cancer therapeutics, 20(4), 676-690.

+ Open protocol

+ Expand

Quantifying Apoptosis and Proliferation

Check if the same lab product or an alternative is used in the 5 most similar protocols

CC3 expression levels were assessed by flow cytometry after plating 20,000 cells/well in 96-well, round-bottom plates (Corning) and then treating them with or without 2 μg/mL dox (Sigma) and DMSO (Sigma) or 10 nM AC220 (Selleckchem) for 48 hours. Fixed and permeabilized cells were stained with a CC3 Ab (BD Horizon; catalog 560627) for 1 hour and analyzed on the Attune NxT flow cytometer (Thermo Fisher) at the UCSF HDFCCC LCA Core Facility. Annexin V and EdU were similarly assessed by flow cytometry after treating with dox and AC220 for 48 and 24 hours, respectively, then following the staining protocols as specified by their respective manufacturers: Annexin V Apoptosis Detection Kit (BD 556547) and Click-iT Plus EdU Flow Cytometry Assay Kit (Thermo Fisher C10634).

+ Open protocol

+ Expand

Preparation and Storage of Anticancer Agents

Check if the same lab product or an alternative is used in the 5 most similar protocols

GO (Mylotarg, Pfizer, New York, NY) was diluted in deionized water, and cytarabine (Pfizer, New York, NY) and daunorubicin (Teva Parenteral Medicines) were diluted with phosphate-buffered saline and stored at 4°C before usage. 8.8 ADC is a nonbinding antibody conjugated to N-Ac-γ-calicheamicin DMH (Pfizer, New York, NY). AC220 was purchased from Selleck Chemicals (Houston, TX) and prepared as previously described [9] (link).

Zhang C.C., Yan Z., Pascual B., Jackson-Fisher A., Huang D.S., Zong Q., Elliott M., Fan C., Huser N., Lee J., Sung M, & Sapra P. (2017). Gemtuzumab Ozogamicin (GO) Inclusion to Induction Chemotherapy Eliminates Leukemic Initiating Cells and Significantly Improves Survival in Mouse Models of Acute Myeloid Leukemia. Neoplasia (New York, N.Y.), 20(1), 1-11.

+ Open protocol

+ Expand

Cell Line Culture Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols

The Ba/F3, Hek-293T, and WEHI-3B cell lines were obtained from DSMZ (Braunschweig, Germany), the U2OS cell line from ATCC (Wesel, Germany) and cultured according to the supplier’s recommendation. The retroviral packaging cell line Phoenix eco was purchased from Orbigen (San Diego, CA, USA). Recombinant human FLT3 ligand (FL) was obtained from PromoKine (Heidelberg, Germany), recombinant murine IL-3 from ImmunoTools (Friesoythe, Germany) and AC220 was obtained form Selleck Chemicals (Houston, TX, USA).

Sandhöfer N., Bauer J., Reiter K., Dufour A., Rothenberg M., Konstandin N.P., Zellmeier E., Tizazu B., Greif P.A., Metzeler K.H., Hiddemann W., Polzer H, & Spiekermann K. (2016). The new and recurrent FLT3 juxtamembrane deletion mutation shows a dominant negative effect on the wild-type FLT3 receptor. Scientific Reports, 6, 28032.

+ Open protocol

+ Expand

Compound Screening in Cancer Research

Check if the same lab product or an alternative is used in the 5 most similar protocols

Drugs and suppliers used in the study were as follows: 17-AAG, rapamycin, sorafenib and torin1 from LC labs (www.lclabs.com); AC220, JQ1, selinexor, tosedostat, TW-37 and vosaroxin from Selleck (supplied by Stratech UK); ABT-199 from Adooq, www.adooq.com; ABT-737 from Sequoia, Pangbourne, UK; A-1210477 from Chemie Tek, www.chemietek.com; etoposide from Tocris, Bristol, UK; Mylotarg from Wyeth, Pearl River USA; Pladienolide B from Santa Cruz, supplied by Insight, Wembley, UK; TG02 was from Tragara, San Diego, USA. Other drugs and reagents were from Sigma (Poole, Dorset, UK) unless specified.

Grundy M., Seedhouse C., Jones T., Elmi L., Hall M., Graham A., Russell N, & Pallis M. (2018). Predicting effective pro-apoptotic anti-leukaemic drug combinations using co-operative dynamic BH3 profiling. PLoS ONE, 13(1), e0190682.

+ Open protocol

+ Expand

FLT3 Inhibitor Combination Therapy Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

E6201 was provided by Eisai Inc. (Woodcliff Lake, New Jersey), sorafenib and AC220 (quizartinib) were purchased from Selleckchem (Houston, TX). The chemical structures of these agents are shown at Supplementary Figure S1. Recombinant human FLT3/FLK2 ligand (FL) was purchased from R&D (Minneapolis, MN). Interleukin-3 (IL-3) was purchased from PEPROTECH (Rocky Hill, NJ). The antibodies against human phosphorylated (p)-p44/42 MAPK (ERK1/2)(Thr202/Tyr204), phospho-AKT(Ser473), phospho-FLT3(Tyr589/591), phospho-S6K(Ser240/244), phospho-MEK1/2, AKT, S6K, Bcl-xL, and cleaved-caspase-3 were purchased from Cell Signaling Technology (Danvers, MA), against Bcl-2 from Dako (Carpinteria, CA), against phospho-STAT5 A/B from Upstate (Lake Placid, NY), against ERK2, FLT3, p53 and Mcl-1 from Santa Cruz Biotechnology (Santa Cruz, CA), against Bim and Puma from CalBiochem (San Diego, CA), and against Ki67 was purchased from Abcam (Cambridge, MA). The anti-luciferase antibody was purchased from Promega (Madison, WI).

Zhang W., Borthakur G., Gao C., Chen Y., Mu H., Ruvolo V., Nomoto K., Zhao N., Konopleva M, & Andreeff M. (2016). The dual MEK/FLT3 inhibitor E6201 exerts cytotoxic activity against acute myeloid leukemia cells harboring resistance-conferring FLT3 mutations. Cancer research, 76(6), 1528-1537.

+ Open protocol

+ Expand

Intravitreal Injection of AC220 for Neovascularization

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

AC220, a second-generation Flt3 inhibitor, was purchased from Selleck (Selleckchem, Houston, TX) [23 (link), 24 (link)]. Mice received intravitreal injections (1 μl) of AC220 and phosphate-buffered saline (PBS) following laser photocoagulation (day 0, D0). This was repeated at 7 days. One microliter AC220 was administered into the vitreous cavity of murine eyes using glass micropipette needles at D0 and D7. Eye analysis was performed at 14 days in order to quantify the area of CNV.

Gao Y., Zhong Y., Zhu Y., Demetriades A.M., Cai Y., Shen J., Lu Q., Shen X, & Xie B. (2018). Flt3 Regulation in the Mononuclear Phagocyte System Promotes Ocular Neovascularization. Journal of Ophthalmology, 2018, 2518568.

+ Open protocol

+ Expand

Comprehensive Drug Screening Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Drugs and suppliers used in the study were as follows: 17-AAG, rapamycin, sorafenib, U0126 and torin 1 from LC labs (www.lclabs.com); AC220 and vosaroxin from Selleck (supplied by Stratech UK); etoposide from Tocris; gemtuzumab ozogamicin (GO) was a gift from Wyeth, Pearl River USA. C2 ceramide and Calyculin A were from Santa Cruz Biotechnology, Santa Cruz, CA, USA. Ly294006 was from Millipore, Watford, UK. Other drugs and reagents were from Sigma (Poole, Dorset, UK) unless specified.

Grundy M., Jones T., Elmi L., Hall M., Graham A., Russell N, & Pallis M. (2018). Early changes in rpS6 phosphorylation and BH3 profiling predict response to chemotherapy in AML cells. PLoS ONE, 13(5), e0196805.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!