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Anti sxbp1

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Anti-sXBP1 is a monoclonal antibody that specifically binds to the spliced form of the transcription factor X-box binding protein 1 (XBP1). XBP1 is a key regulator of the unfolded protein response, which is activated during endoplasmic reticulum stress.

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Lab products found in correlation

Stat3, by ABclonal (2 mentions) Supersignal west femto max, by Thermo Fisher Scientific (2 mentions) Supersignal west pico max chemiluminescent substrate, by Thermo Fisher Scientific (2 mentions) Anti sxbp1, by Cell Signaling Technology (2 mentions) Phospho y705 stat3, by ABclonal (2 mentions) Anti ugcg, by Abcam (2 mentions) Anti ire1, by Cell Signaling Technology (2 mentions) Anti p erk, by Cell Signaling Technology (2 mentions) Complete protease inhibitor cocktail, by Roche (2 mentions) Anti atf4, by Santa Cruz Biotechnology (2 mentions) Enhanced chemi luminescence (ecl), by Cytiva (1 mentions) Anti β catenin, by BD (1 mentions) Anti β actin, by Abcam (1 mentions) Anti chop, by Cell Signaling Technology (1 mentions) Anti tubulin, by Santa Cruz Biotechnology (1 mentions) Anti lc3, by MBL Life Science (1 mentions)

Spelling variants of this product

SXBP1 (4 citations)

3 protocols using anti sxbp1

1

Protein Extraction and Western Blot Analysis

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In brief, treated cells were harvested in RIPA buffer (1% Triton X-100, 150mM NaCl, 20mM Hepes (pH 7.5), 10% glycerol, 1mM EDTA, 100mM NaF, 17.5mM β-glycerophosphate, 1mM PMSF, 4µg/ml aprotinin, 2µg/ml pepstatin A), lysates were sonicated and cleared by centrifugation, protein concentrations were quantitated. 25–50 µg were electrophoresed on a reducing Tris-Tricine gel, and electroblotted to PVDF membrane. Antibodies used were anti-β-catenin and anti-Wnt16 (BD Biosciences Pharmingen), anti-p-eIF2#, anti-CHOP (Cell Signaling Technology), anti-LC3 (MBL International), anti-sXbp1 (Biolegend), anti-β-actin (Abcam) and anti-tubulin (Santa Cruz Biotechnology). Primary antibodies were detected with species-specific HRP-secondary antibodies (Invitrogen) and visualized with ECL (Amersham).
Papandreou I., Verras M., McNeil B., Koong A.C, & Denko N.C. (2015). Plant Stilbenes Induce Endoplasmic Reticulum Stress and Their Anti-Cancer Activity can be Enhanced by Inhibitors of Autophagy. Experimental cell research, 339(1), 147-153.
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2

Protein Extraction and Immunoblotting Assay

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Protein extraction was performed using RIPA lysis buffer (50 mM Tris HCl pH 7.4, 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 2mM EDTA) supplemented with Complete Protease Inhibitor Cocktail (Roche) and sodium orthovanadate. After incubation on ice for 30 minutes, lysates were centrifugated 15 minutes at 4°C to remove cellular debris. Protein concentration was determined and then subjected for immunoblotting assay. The signal was visualized with Supersignal West Femto max or Supersignal West Pico max chemiluminescent substrate (Thermo Scientific) with a digital imager. The following primary antibodies were used: anti-sXBP1(D2C1F-Cell Signaling), anti-sXBP1 (6195-BioLegend), phospho-Y705 STAT3 (AP0070-Abclonal), STAT3 (A1192-Abclonal), monoclonal anti-bactin (A2228-Sigma), anti-Tubulin (AG-27B-005 Adipogen), anti-UGCG (ab124296-Abcam), anti-PERK (C33E10-Cell Signaling), anti-IRE1 (14C10-Cell Signaling), anti-ATF4 (sc-200, Santa Cruz).
Di Conza G., Tsai C.H., Gallart-Ayala H., Yu Y.R., Franco F., Zaffalon L., Xie X., Li X., Xiao Z., Raines L.N., Falquet M., Jalil A., Locasale J.W., Percipalle P., Masson D., Huang S.C., Martinon F., Ivanisevic J, & Ho P.C. (2021). Tumor-induced reshuffling of lipid composition on the ER membrane sustains macrophage survival and pro-tumorigenic activity. Nature immunology, 22(11), 1403-1415.
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3

Protein Extraction and Immunoblotting Assay

Check if the same lab product or an alternative is used in the 5 most similar protocols
Protein extraction was performed using RIPA lysis buffer (50 mM Tris HCl pH 7.4, 150 mM NaCl, 1% NP-40, 0.5% sodium deoxycholate, 0.1% SDS, 2mM EDTA) supplemented with Complete Protease Inhibitor Cocktail (Roche) and sodium orthovanadate. After incubation on ice for 30 minutes, lysates were centrifugated 15 minutes at 4°C to remove cellular debris. Protein concentration was determined and then subjected for immunoblotting assay. The signal was visualized with Supersignal West Femto max or Supersignal West Pico max chemiluminescent substrate (Thermo Scientific) with a digital imager. The following primary antibodies were used: anti-sXBP1(D2C1F-Cell Signaling), anti-sXBP1 (6195-BioLegend), phospho-Y705 STAT3 (AP0070-Abclonal), STAT3 (A1192-Abclonal), monoclonal anti-bactin (A2228-Sigma), anti-Tubulin (AG-27B-005 Adipogen), anti-UGCG (ab124296-Abcam), anti-PERK (C33E10-Cell Signaling), anti-IRE1 (14C10-Cell Signaling), anti-ATF4 (sc-200, Santa Cruz).
Di Conza G., Tsai C.H., Gallart-Ayala H., Yu Y.R., Franco F., Zaffalon L., Xie X., Li X., Xiao Z., Raines L.N., Falquet M., Jalil A., Locasale J.W., Percipalle P., Masson D., Huang S.C., Martinon F., Ivanisevic J, & Ho P.C. (2021). Tumor-induced reshuffling of lipid composition on the ER membrane sustains macrophage survival and pro-tumorigenic activity. Nature immunology, 22(11), 1403-1415.
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