Raman station 400f

In order to confirm the presence of particular chemical moieties in MRSA^MupS as well as MRSA^MupRL cells, FTIR and Raman spectroscopy analyses were carried out. After the cultivation of these strains for 18 h at 37 °C on Columbia agar with 5% sheep blood, the cells were washed three times by 5 mL of PBS, centrifuged at 5000 for 5 min, and dried for 24 h at 37 °C. The FTIR spectra of bacterial cells dry samples were obtained at room temperature by attenuated total reflection with a FTIR spectrometer (Perkin Elmer Spectrophotometer 100, Waltham, MA, USA). The samples (100 mg) were then scanned at a range between 650 cm⁻¹ and 4000 cm⁻¹ (64 scans and 1 cm⁻¹ resolution). The obtained spectra were normalized, baseline corrected, and analyzed using SPECTRUM software (v10, Perkin Elmer Spectrophotometer, Waltham, MA, USA).
To obtain Raman spectra, the samples were analyzed using a Raman spectrometer (RamanStation 400F, Perkin Elmer, USA) with point and shot capability using an excitation laser source at 785 nm (to avoid fluorescence excitation), 100 micron spot size, 4 shots, and 8 s exposition time. The obtained spectra were normalized, baseline corrected, and analyzed using SPECTRUM software (v10, Perkin Elmer, Waltham, MA, USA).

All the SEM and EDS results are derived from the microscope (FEI, Quanta 450) integrated with the instrument (INCA Energy 200). The contact angles are measured by the goniometer (OCA25, Dataphysics). The crystallinity is characterized by the XRD (Rigaku Smartlab) and the Raman spectrometer (Perkinelmer Raman station 400F). The ferroelectricity is conducted on an Asylum Research MFP-3D Infinity AFM.

In order to confirm the presence of particular chemical moieties in K. pneumoniae^Col-S and K. pneumoniae^Col-R, as well as in their LPS, FTIR and Raman spectroscopic analyses, were performed [18 (link)]. After 18 h cultivation of each strain at 37 °C on Columbia agar with 5% sheep blood, bacterial colonies were harvested, transferred to an Eppendorf tube, and washed three times using saline. Then, the samples were centrifuged at 5000× g for 5 min and dried for 24 h at 37 °C. The FTIR spectra of dried bacterial cell samples and freeze-dried LPS were obtained at room temperature by an attenuated total reflection FTIR spectrometer (Perkin Elmer Spectrophotometer 100, Waltham, MA, USA). The samples (100 mg) were then scanned at a range between 650 cm⁻¹ and 4000 cm⁻¹ (64 scans and 1 cm⁻¹ resolution). The obtained spectra were normalised, baseline corrected, and analysed using SPECTRUM software (v10, Perkin Elmer Spectrophotometer, Waltham, MA, USA).
To obtain Raman spectra, the samples were analysed using the Raman spectrometer (RamanStation 400F, Perkin Elmer, Waltham, MA, USA) with point and shot capability and excitation laser source at 785 nm (to avoid fluorescence excitation), 100-micron spot size, and 4 scans (8 s exposition time). The obtained spectra were normalised, baseline corrected and analysed using SPECTRUM software (v10, Perkin Elmer, Waltham, MA, USA).