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Ab108791

Manufactured by Abcam
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Sourced in United Kingdom, United States

Ab108791 is a lab equipment product offered by Abcam. It serves a core function, but a detailed description cannot be provided while maintaining an unbiased and factual approach without extrapolation.

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Lab products found in correlation

Ab105134, by Abcam (2 mentions) Ab105135, by Abcam (2 mentions) Bead beater, by Biospec (1 mentions) Protein assay, by Bio-Rad (1 mentions) Vegf165, by R&D Systems (1 mentions) Protease and phosphatase inhibitor, by Roche (1 mentions) Hepatocyte growth factor (hgf), by R&D Systems (1 mentions) Ab151276, by Abcam (1 mentions) Ab83369, by Abcam (1 mentions)

7 protocols using ab108791

1

Quantifying Mouse Albumin by ELISA

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Mouse albumin levels were determined by ELISA using a commercially available kit as per the company’s instructions (Abcam, ab108791). Samples were normalized for protein concentration using the BCA Assay.
Hoffman J.B., Petriello M.C., Morris A.J., Mottaleb M.A., Sui Y., Zhou C., Deng P., Wang C, & Hennig B. (2020). Prebiotic inulin consumption reduces dioxin-like PCB 126-mediated hepatotoxicity and gut dysbiosis in hyperlipidemic Ldlr deficient mice. Environmental pollution (Barking, Essex : 1987), 261, 114183.
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2

Vitreous Albumin Quantification in Murine Models

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Vitreous humour was collected from the eyes of adult wild-type mice, P17 normoxic mice, P17 mice with OIR, adult wild type mice with choroidal NV, P30 rho/VEGF mice, Tet/Opsin/VEGF mice at 2 or 3 days after addition of doxycycline to drinking water (2 mg/ml). Using the manufacturer’s instructions, a mouse albumin ELISA kit (ab108791; Abcam, Cambridge, MA) was used to measure albumin levels in 1 µL of diluted vitreous humour and albumin samples for standard curve generation. The plate was read at 450 nm and 570 nm.
Fortmann S.D., Lorenc V.E., Shen J., Hackett S.F, & Campochiaro P.A. (2018). Mousetap, a Novel Technique to Collect Uncontaminated Vitreous or Aqueous and Expand Usefulness of Mouse Models. Scientific Reports, 8, 6371.
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3

Visualizing Blood-Brain Barrier Permeability in HSV-1 Encephalitis

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Following infection mice were examined twice daily using an established score of murine neurological morbidity due to HSV-1 encephalitis (Thomas et al., 2001 (link)). To visualize BBB permeability, mice were anaesthetized with ketamine/xylazine and injected intravenously with 100ul of 2% Evans blue in sterile PBS 30 min prior to euthanasia following a modified version of previously established methodology (Bennett et al., 2010 (link); Shen et al., 2019 (link)). Mice were then euthanized with CO2 and death confirmed by transection of the great arteries. A craniotomy was performed and images taken. For the assessment of BBB permeability using the brain to serum albumin ratio, a cardiac puncture was performed to extract blood immediately after euthanasia performed with CO2. The blood was left to clot for 30 min at 4°C and centrifuged at 5,000 rpm for 5 min to obtain serum. A craniotomy was performed and brains were homogenized in PBS using a BeadBeater (Biospec) followed by a centrifugation at 15,000 rpm for 5 min. The levels of albumin in serum and brain was assessed by performing an ELISA on the serum and brain supernatants following the manufacturer’s instructions (Cat ab108791, Abcam).
Michael B.D., Bricio-Moreno L., Sorensen E.W., Miyabe Y., Lian J., Solomon T., Kurt-Jones E.A, & Luster A.D. (2020). Astrocyte- and Neuron-Derived CXCL1 Drives Neutrophil Transmigration and Blood-Brain Barrier Permeability in Viral Encephalitis. Cell reports, 32(11), 108150.
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4

Intraocular Injection of HGF and VEGF

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Adult C57BL/6 mice were anesthetized and had intraocular injection of 1 μL of 6.25 μmol/L HGF or VEGF165 (R&D Systems) in one eye and PBS in the fellow eye. At 1 and 3 days after injection, some of the mice were anesthetized and chests were opened to expose the heart; a 27‐gauge cannula was inserted into left ventricle, the right atrium was cut for drainage, and the vasculature was flushed with PBS for 3 minutes, and retinas were dissected intact. Retinas or vitreous samples were sonication in PBS/0.3% Triton buffer containing phosphatase and protease inhibitors (Roche), centrifugation at 9200 g for 10 minutes, and supernatants were stored at −20°C. Albumin levels in retinal or vitreous samples were measured using the albumin ELISA kit (ab108791, Abcam). The protein concentrations of retinal lysates were measured by the BioRad protein assay and 2 μg of total protein was added to each well. One μL of each vitreous sample was added to each well. Plates were read at 450 and at 570 nm and albumin levels were determined by plotting values on a standard curve.
Lorenc V.E., Lima e Silva R., Hackett S.F., Fortmann S.D., Liu Y, & Campochiaro P.A. (2020). Hepatocyte growth factor is upregulated in ischemic retina and contributes to retinal vascular leakage and neovascularization. FASEB BioAdvances, 2(4), 219-233.
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5

Assessing Liver Toxicity after AAV

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To assess whether AAV administration caused liver toxicity, levels of mouse plasma albumin and the activities of plasma alanine transaminase (ALT) and aspartate aminotransferase (AST) were measured using kits from Abcam (Cambridge, UK; Cat #ab108791 for albumin, #ab105134 for ALT, #ab105135 for AST). All assays were performed according to the manufacturer’s instructions.
Chen H., Shi M., Gilam A., Zheng Q., Zhang Y., Afrikanova I., Li J., Gluzman Z., Jiang R., Kong L.J, & Chen-Tsai R.Y. (2019). Hemophilia A ameliorated in mice by CRISPR-based in vivo genome editing of human Factor VIII. Scientific Reports, 9, 16838.
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6

Quantifying Plasma Albumin Levels

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Plasma was collected in EDTA-coated tubes, aliquoted, and frozen at −80°C for further analysis. Plasma mouse albumin levels were determined using a mouse albumin ELISA assay according to manufacturers’ specification (Abcam #ab108791).
Davidson S.M., Jonas O., Keibler M.A., Hou H.W., Luengo A., Mayers J.R., Wyckoff J., Del Rosario A., Whitman M., Chin C.R., Condon K.J., Lammers A., Kellersberger K.A., Stall B.J., Stephanopoulos G., Bar-Sagi D., Han J., Rabinowitz J.D., Cima M., Langer R, & Vander Heiden M.G. (2016). Direct evidence for cancer cell-autonomous extracellular protein catabolism in pancreatic tumors. Nature medicine, 23(2), 235-241.
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7

Serum Biomarkers of Liver and Fibrosis

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Mouse serum alanine aminotransferase (AST), aspartate aminotransferase (ALT), and alkaline phosphatase (ALP) activities were measured using specific assay kits (ab105134 for AST, ab105135 for ALT, ab83369 for AP, Abcam, Shanghai, China). Serum hyaluronic acid (HA), N-terminal propeptide of collagen III (PIIINP), albumin (ALB), and globulin (GLOB) levels were analyzed with commercial ELISA kits (CSB-E08121m for HA, CUSABIO, Houston, TX, USA; LS-F25207 for PIIINP, LSBio, Seattle, WA; ab108791 for albumin, and ab151276 for globulin, Abcam, Shanghai, China).
Huang Y., Lu J., Xu Y., Xiong C., Tong D., Hu N, & Yang H. (2020). Xiaochaihu decorction relieves liver fibrosis caused by Schistosoma japonicum infection via the HSP47/TGF-β pathway. Parasites & Vectors, 13, 254.
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