Bumetanide and all of the other compounds (with the exception of AR-C155858) were purchased from Sigma-Aldrich (St. Louis, MO). AR-C155858 was purchased from Tocris Bioscience (Bristol, U.K.). [3H]Bumetanide (15–30 Ci/mmol) was purchased from American Radiolabeled Chemicals (St. Louis, MO). GIBCO Leibovitz’s L-15 medium with glutamine (Cat. No. 41300-039) and all Western blotting materials were supplied by Thermo Fisher Scientific (Rockford, IL). The pCR2.1-TOPO, TOPO TA cloning kit, TRIzol Reagent, and mMESSAGE mMACHINE T7 transcription kit were purchased from Thermo Fisher Scientific (Rockford, IL). pGH19 vector was kindly provided by Dr. Walter F. Boron (Case Western Reserve University, Department of Physiology and Biophysics, Cleveland, Ohio).24 (link) The FlashGel System was purchased from Lonza (Portsmouth, NH). All enzymes were purchased from New England Biotechnology (Ipswich, MA). The gel extraction and PCR purification kits were purchased from Qiagen (Valencia, CA). DNA purity and concentration were verified using a NanoDrop 1000 instrument (Thermo Fisher Scientific, Rockford, IL). A ZOE fluorescent cell imager made by Bio-Rad (Hercules, CA) was used for fluorescent microscopy. The mouse anti-Egfp antibody (JL-8, Cat. No. 632381) was purchased from Clontech (Mountain View, CA).
Gel extraction and pcr purification kits
Manufactured by Qiagen
Sourced in Germany
Gel extraction and PCR purification kits are designed to extract and purify DNA fragments from agarose gels and PCR reactions. They allow for the efficient recovery of DNA samples while removing contaminants that may interfere with downstream applications.
Automatically generated - may contain errors
Lab products found in correlation
Trizol reagent, by Thermo Fisher Scientific (2 mentions)
Topo ta cloning kit, by Thermo Fisher Scientific (2 mentions)
Nanodrop 1000 instrument, by Thermo Fisher Scientific (2 mentions)
Flashgel system, by Lonza (2 mentions)
Bumetanide, by Merck Group (2 mentions)
Mmessage mmachine t7 transcription kit, by Thermo Fisher Scientific (2 mentions)
T4 dna ligase, by New England Biolabs (2 mentions)
Plasmid miniprep kits, by Metabion (2 mentions)
Ni nta agarose, by Macherey-Nagel (2 mentions)
Porphyrins, by Frontier Scientific (2 mentions)
Oligonucleotide primers, by Metabion (2 mentions)
Ar c155858, by Merck Group (1 mentions)
Zoe fluorescent cell imager, by Bio-Rad (1 mentions)
Pcr2.1 topo, by Thermo Fisher Scientific (1 mentions)
Ar c155858, by Bio-Techne (1 mentions)
First strand cdna synthesis kit, by Takara Bio (1 mentions)
M13k07 helper phage, by New England Biolabs (1 mentions)
Freund s adjuvant, by Merck Group (1 mentions)
Sfii restriction enzyme, by New England Biolabs (1 mentions)
Probenecid, by Merck Group (1 mentions)
8 protocols using gel extraction and pcr purification kits
1
Bumetanide Transport Assay Protocol
2
Synthesis and Characterization of Carbofuran Analogues
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The haptens 4-[[(2,3-dihydro-2,2-dimethyl-7-benzofuranyloxy) carbonyl]-amino] butanoic acid (BFNB) was previously synthesized in the laboratory [22 (link)]. Carbofuran pesticide and its structural analogues were purchased from Hua Xin Bio. Co. Ltd. (Tianjin, China). Keyhole limpet hemocyanin (KLH), ovalbumin (OVA), and Freund’s adjuvant were obtained from Sigma-Aldrich (St. Louis, MO, USA). The TRIzol reagent was purchased from Thermo Fisher Scientific (Shanghai, China). The first strand cDNA synthesis kit was obtained from TaKaRa (Dalian, China). Gel extraction and PCR purification kits were purchased from QIAGEN (Dusseldorf, Germany). Helper phage M13K07, SfiI restriction enzymes, and T4 DNA ligase were purchased from New England Biolabs (Beijing, China). The anti-HA tag antibody (HRP) was obtained from NOVUS Biologicals (Shanghai, China). Primary secondary amine (PSA), graphitized carbon black (GCB), C18 sorbents, and PestiCarb (PC) were purchased from Biocomma Limited (Shenzhen, China).
3
Protocol for Cloning and Expression of Murine Slc16a5
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Prostaglandin F2α and other compounds were purchased from Cayman Chemical (Ann Arbor, MI, USA). All ELISAs were also purchased from Cayman Chemical. [3H]PGF2α was purchased from Perkin Elmer (Waltham, MA, USA). GIBCO Leibovitz’s L-15 medium with glutamine (Cat. # 41300-039), and all Western Blotting materials were supplied by Thermo Fisher Scientific (Rockford, IL, USA). For cloning purposes, complementary DNA (cDNA) encoding for murine Slc16a5 (NM_001080934.1), TOPO® TA cloning kit, and mMESSAGE mMACHINE T7 transcription kit were purchased from Thermo Fisher Scientific. For DNA isolation, a REDExtract-N-Amp™ Tissue Polymerase Chain Reaction Kit was purchased from Sigma Aldrich (St. Louis, MO, USA). Bumetanide and probenecid were also purchased from Sigma Aldrich. The FlashGel™ System was purchased from Lonza (Portsmouth, NH, USA). All enzymes were purchased from New England Biotechnology (Ipswich, MA, USA). The Gel Extraction and PCR Purification kits were purchased from Qiagen (Valencia, CA, USA). DNA purity and concentration were verified using a NanoDrop 1000 instrument (Thermo Fisher Scientific, Rockford, IL, USA). The rabbit polyclonal anti-FLAG antibody (Cat. # ab1162) was purchased from Abcam (Cambridge, MA, USA).
4
Chemical Reagent and Enzyme Sources
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Chemicals were purchased from Merck, Qualigens, or Sigma-Aldrich (USA) unless specified otherwise. Enzymes were from Bangalore Genei (India), MBI Fermentas (USA) or New England Biolabs (USA). Gel extraction and PCR purification kits were from Qiagen. Primers were synthesized by GCC Biotech (India).
5
Cloning and Expression of Methanogenic Genes
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All chemicals and reagents were obtained from Sigma-Aldrich (Taufkirchen, Germany), Carl Roth (Karlsruhe, Germany), Gerbu (Heidelberg, Germany), Merck (Darmstadt, Germany). DNA polymerases, restriction enzymes, and PCR requisites were purchased from New England Biolabs (Frankfurt a.M., Germany). Oligonucleotide primers and plasmid-miniprep kits were purchased from Metabion (Martinsried, Germany). PCR purification and gel extraction kits were obtained from Qiagen (Hilden, Germany). Ni-NTA agarose was purchased from Macherey-Nagel (Düren, Germany). Porphyrins were purchased from Frontier Scientific (Newark, USA). The four E. coli codon-optimized genes Mbar_A1459, _A1460, _A1458, and _A1793 from M. barkeri were synthesized by Life Technologies GmbH (Darmstadt, Germany).
6
Molecular Cloning: A Comprehensive Guide
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Phusion® High-Fidelity DNA Polymerase, DNA marker, Taq DNA polymerase, and T4 DNA ligase were purchased from New England Biolabs, and cloning kits from Qiagen. pET22b, pcDNA™ 3.1 (+), and host strain Escherichia coli DH5α were obtained from Invitrogen. Human cDNAs were purchased from Clontech. Oligonucleotide primers were purchased from Invitrogen. PCR purification and gel extraction kits were purchased from Qiagen. Plasmids were isolated using a QIAprep Spin Miniprep Kit (Qiagen). All other chemicals used in the study were of molecular biology grade.
7
Molecular Cloning of Key Transcription Factors
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Human LIF, mouse Hand2, T-box 5 and MEF2C genes were cloned into the plasmid pRSET-B. A luciferase shRNA cDNA fragment was cloned into the plasmid pLV-U6-EGFP. Restriction enzymes, T4 DNA ligase and qPCR kits were purchased from Thermo Scientific Co. PCR purification and gel extraction kits were obtained from Qiagen, Axygen (Corning), Tiangen and CWBiotech, and DH5α competent cells were purchased from Bio Swamp Co. Chemicals were purchased from the Chinese Chemical Reagent Co.
8
Purification and Characterization of Mbar_A1458
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All chemicals and reagents were obtained from Sigma-Aldrich (Taufkirchen, Germany), Carl Roth (Karlsruhe, Germany), Gerbu (Heidelberg, Germany), Merck (Darmstadt, Germany). DNA polymerases, restriction enzymes, PCR requisites as well as the Q5 Site-Directed Mutagenesis Kit were purchased from New England Biolabs (Frankfurt a. M., Germany). Oligonucleotide primers and plasmid-miniprep Kits were purchased from Metabion (Martinsried, Germany). PCR purification and gel extraction Kits were obtained from Qiagen (Hilden, Germany). Ni-NTA agarose was purchased from Macherey-Nagel (Düren, Germany). Porphyrins were all purchased from Frontier Scientific (Logan, UT, USA) except of Zn-coproporphyrin III. The for E. coli codon-optimized gene Mbar_A1458 from M. barkeri was synthesized by Life Technologies GmbH (Darmstadt, Germany).
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