Antibiotic treatment was given in the drinking water and contained ampicillin (PanReac AppliChem A0839, 0025) 1 g/L + neomycin (Biological Industries 1405-10-3) 0.5 g/L. ampicillin and neomycin were chosen because ampicillin is active against Gram-positive and some Gram-negative bacteria, while neomycin has excellent activity against Gram-negative bacteria and has partial activity against Gram-positive bacteria. In addition, both antibiotics are poorly absorbed (or unabsorbed as in the case of neomycin) and thus have no systemic effects [22 (link)].
Neomycin
Manufactured by Sartorius
Sourced in Israel
Neomycin is a laboratory product used for various applications in the field of microbiology and cell culture. It is an antibiotic that can be used for the selection and maintenance of genetically modified cells or organisms in research environments. The core function of Neomycin is to inhibit the growth of certain bacterial strains, allowing for the cultivation and isolation of desired cell lines or microbial cultures.
Automatically generated - may contain errors
Lab products found in correlation
Penicillin, by Sartorius (4 mentions)
Streptomycin, by Sartorius (4 mentions)
L glutamine, by Sartorius (3 mentions)
Dmem f12 medium, by Sartorius (1 mentions)
Foetal bovine serum (fbs), by Sartorius (1 mentions)
Mnng hos, by Sartorius (1 mentions)
Rpmi 1640 medium, by Sartorius (1 mentions)
Dmem medium, by Sartorius (1 mentions)
Penicillin streptomycin, by Sartorius (1 mentions)
Fetal bovine serum (fbs), by Sartorius (1 mentions)
Ampicillin, by ITW Reagents (1 mentions)
Ampicillin, by Sartorius (1 mentions)
Rpmi 1640, by Merck Group (1 mentions)
Dulbecco s modified eagle s medium, by Merck Group (1 mentions)
Hek293t human embryonic kidney cells, by American Type Culture Collection (1 mentions)
4t1 murine breast carcinoma cells, by American Type Culture Collection (1 mentions)
Mda mb 231 human breast carcinoma cells, by American Type Culture Collection (1 mentions)
Dulbecco modified eagle medium (dmem), by Sartorius (1 mentions)
Fetal calf serum (fcs), by Sartorius (1 mentions)
4t1 murine mammary adenocarcinoma, by American Type Culture Collection (1 mentions)
6 protocols using neomycin
1
Antibiotic Treatment in Drinking Water
2
Culturing Osteoblastic Cell Lines
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Human foetal osteoblastic cell line hFOB 1.19 and human OS cell lines MNNG/HOS and Saos2 were obtained from the Typical Culture Preservation Committee of the Chinese Academy of Sciences, Shanghai, China. MG63, 143B and U2OS cell lines were purchased from American Type Culture Collection (Manassas, VA, USA). hFOB 1.19 cells were maintained in DMEM/F12 medium containing 10% foetal bovine serum (FBS, Biological Industries, Israel) and 300 mg/ml neomycin (G418) in 5% CO2 atmosphere at 34°C. Saos2 cells were incubated in RPMI 1640 medium (Biological Industries, Israel) and U2OS, MNNG/HOS, 143B and MG63 cells in DMEM medium (Biological Industries, Israel) containing 10% FBS and 1% penicillin/streptomycin (Biological Industries, Israel) in an atmosphere of 5% CO2 at 37°C.
3
Culturing Actin-GFP 3T3 Fibroblasts
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Actin-GFP 3T3 fibroblast cells (a gift from Prof. Scott E. Fraser, USC, Los Angeles, CA) were cultured in DMEM supplemented with 10% fetal bovine serum, nonessential amino acids, sodium pyruvate, l-glutamine, 100 units/ml penicillin, 100 μg/ml streptomycin, and 100 μg/ml neomycin (all the materials supplied by Biological Industries, Kibbutz Beit Haemek, Israel), in a 37°C humid incubator.
4
Cell lines and culture conditions
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MDA-MB-231 human breast carcinoma cells, 4T1 murine breast carcinoma cells, and HEK293T human embryonic kidney cells were purchased from the American Type Culture Collection (ATCC). The LM2-4 cell line, a lung metastatic variant of the MDA-MB-231 cell line, was kindly provided by Prof. Robert Kerbel (Sunnybrook Health Sciences Centre, Toronto, Ontario, Canada). 67NR, a low metastatic variant of the 4T1 cell line, was kindly provided by Prof. Jonathan Sleeman (Medical Faculty Mannheim, Centre for Biomedicine and Medical Technology Mannheim (CBTM), University of Heidelberg, Mannheim 68167, Germany). In some cases, cells were fluorescently tagged with green fluorescent protein (GFP). All cells were used within 6 months of resuscitation. Human cell lines were cultured in RPMI 1640 (Sigma-Aldrich, Rehovot, Israel) and murine cell lines in Dulbecco’s modified Eagle’s medium (Sigma-Aldrich). In all cases, medium was supplemented with 5% fetal bovine serum (FBS), 1% l-glutamine, 1% sodium pyruvate, and 1% streptomycin, penicillin, and neomycin in solution (10 mg/mL, Biological Industries, Beit, Ha’Emek, Israel). Cells were tested routinely to be mycoplasma-free. Human cell lines were tested to be authentic or they were used within 6 months from resuscitation.
5
Murine and Human Cancer Cell Lines
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Murine Lewis lung carcinoma (LLC), EMT/6 and 4T1 mammary adenocarcinoma, and human MCF7 breast carcinoma cell lines were purchased from the American Type Culture Collection (ATCC). All cells were grown in DMEM, supplemented with 10% fetal calf serum, 1% L-glutamine, 1% sodium pyruvate, and 1% streptomycin, penicillin, and neomycin in solution (10 mg/mL, Biological Industries). Some of the cell lines (as indicated below) were stably transfected with GFP or mCherry vectors (Clontech Laboratories, 632379 and 631985, respectively). The cells were passaged in culture for no more than 4 months after being thawed from authentic stocks and were regularly tested and found to be mycoplasma-free.
6
Establishing In Vitro Models for Cancer Research
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Murine LLC, 4T1 murine mammary adenocarcinoma, and A549 human lung carcinoma cell lines were purchased from the American Type Culture Collection (ATCC) and used within 6 months after thawing. LM2-4, a metastatic variant of the MDA-MB-231 human breast carcinoma line (Munoz et al., 2006 (link)), was kindly provided by Dr. R.S. Kerbel (Toronto, ON, Canada). LLC cells were grown in DMEM. 4T1, A549, and LM2-4 cells were grown in RPMI-1640 medium. Culture media were supplemented with 10% fetal calf serum, 1% L-glutamine, 1% sodium pyruvate, and 1% streptomycin, penicillin, and neomycin in solution (10 mg/mL; Biological Industries). LECs were purchased from Lonza and cultured on fibronectin-coated plates (10 μg/mL; Biological Industries) with endothelial cell basal medium (EGM; Lonza).
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