7150 autoanalyzer

Serum total cholesterol (TC) and TG levels were measured with kits on a Hitachi 7150 auto-analyzer (Hitachi Ltd., Tokyo, Japan). After precipitation of serum chylomicrons with dextran sulfate magnesium, the concentrations of low-density lipoprotein cholesterol (LDL-C) and HDL-C in the supernatants were enzymatically measured. Fasting serum glucose levels were measured using a glucose oxidase method with a Beckman Glucose analyzer (Beckman Instruments, Irvine, CA, USA). Insulin and C-peptide levels were measured by radioimmuno-assays with commercial kits (ImmunoNucleo Corporation, Stillwater, MN, USA). IR was calculated with the homeostasis model assessment (HOMA) using the following equation:
It developed by Matthews et al. [19 (link)] and Choi et al. [20 ]. Hemoglobin A1c (Hb_A1c) was measured by a glycated hemoglobin analyzer (SD A1cCare™; SD Biosensor Inc., Suwon, Korea).

Blood samples were collected in plain tubes and ethylenediaminetetraacetic acid (EDTA)-treated tubes in the morning after an 8-hour fast. Blood samples were separated into serum or plasma by a centrifuge, aliquoted and stored at −80°C before analyses. Serum TG concentrations and total cholesterol were measured using kits on a Hitachi 7150 autoanalyzer (Hitachi, Tokyo, Japan). After precipitation of serum chylomicrons with dextran sulfate magnesium, the concentrations of low-density lipoprotein cholesterol (LDL-C) and HDL-C in the supernatant were analyzed enzymatically. The serum glucose concentration was measured using a glucose oxidase method with a Beckman glucose analyzer (Beckman Instruments, Irvine, CA, USA). The glycosylated hemoglobin (HbA1c) concentration was measured using a glycated hemoglobin analyzer (SD A1cCare; SD Biosensor Inc., Suwon, Korea). The serum insulin and C-peptide concentrations were assessed using radioimmunoassay methods. The homeostasis model assessment insulin resistance (HOMA-IR) was calculated using HOMA method developed by Matthews et al.^{15 (link)}

Blood fasting glucose levels were determined by the glucose oxidase method with a Beckman Glucose Analyzer (Beckman Instruments, Irvine, CA, USA). Glycated hemoglobin (Hb_A1c) was measured using a VARIANT II Turbo HbA1C kit-2.0 (Bio-Rad, Hercules, CA, USA). Insulin and C-peptide levels were measured by radioimmunoassays using commercial kits (ImmunoNucleo Corporation, Stillwater, MN, USA). Insulin resistance (IR) was calculated with the homeostatic model assessment (HOMA) using the following equation:
Serum triglyceride, total cholesterol, and low-density lipoprotein (LDL)-cholesterol levels were measured by enzymatic assays using commercially available kits on a Hitachi 7150 Autoanalyzer (Hitachi Ltd., Tokyo, Japan). After precipitation of chylomicrons with dextran sulfate magnesium, the levels of high-density lipoprotein (HDL)-C in the supernatant were analyzed by an enzymatic method. The serum level of high-sensitivity C-reactive protein (hs-CRP) was measured with an ADVIA 1650 system (Bayer Healthcare, Tarrytown, NY, USA) using a commercially available, high-sensitivity CRP-Latex (II) × 2 Kit (Seiken Laboratories Ltd., Tokyo, Japan).