Cholesterol, E1, E2, E3, 4‐hydroxyestrone, 17α‐ethynylestradiol, HIP [also known as 3‐(7a‐methyl‐1,5‐dioxooctahydro‐1H‐inden‐4‐yl) propanoic acid] and testosterone were purchased from Sigma‐Aldrich (St. Louis, Missouri, USA). PEA was prepared as described by Chen et al., 2017 (link). The [3,4C‐13C]E1 (99%) was purchased from Cambridge Isotope Laboratories (Tewksbury, Massachusetts, USA). All other chemicals were of analytical grade and purchased from Honeywell Fluka (Loughborough, UK), Mallinckrodt Baker (Phillipsburg, NJ, USA), Merck (Darmstadt, Germany) and Sigma‐Aldrich.
3 7a methyl 1 5 dioxooctahydro 1h inden 4 yl propanoic acid
Manufactured by Merck Group
Sourced in United States
3‐(7a‐methyl‐1,5‐dioxooctahydro‐1H‐inden‐4‐yl) propanoic acid is a chemical compound. It consists of a cyclopentanoperhydrophenanthrene core structure with a propanoic acid functional group. The compound has a molecular formula of C₁₄H₂₀O₃.
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2 protocols using 3 7a methyl 1 5 dioxooctahydro 1h inden 4 yl propanoic acid
1
Steroid and Lipid Quantification Protocol
2
Synthesis and Characterization of Steroid Compounds
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Chemicals E1, E2, E3, 4-hydroxyestrone, 17α-ethynylestradiol, and HIP, also known as 3-(7a-methyl-1,5-dioxooctahydro-1H-inden-4-yl) propanoic acid, were purchased from Sigma-Aldrich (St.
Louis, Missouri, USA). PEA was prepared as described by Chen et al., 2017. The [3,4C-13 C]E1 (99%) was purchased from Cambridge Isotope Laboratories (Tewksbury, Massachusetts, USA).
All other chemicals were of analytical grade and purchased from Honeywell Fluka (Loughborough, UK), Mallinckrodt Baker (Phillipsburg, NJ, USA), Merck (Darmstadt, Germany), and Sigma-Aldrich (St. Louis, MO, United States).
Louis, Missouri, USA). PEA was prepared as described by Chen et al., 2017. The [3,4C-13 C]E1 (99%) was purchased from Cambridge Isotope Laboratories (Tewksbury, Massachusetts, USA).
All other chemicals were of analytical grade and purchased from Honeywell Fluka (Loughborough, UK), Mallinckrodt Baker (Phillipsburg, NJ, USA), Merck (Darmstadt, Germany), and Sigma-Aldrich (St. Louis, MO, United States).
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