Sigma 1 14 centrifuge

The analysis of HPLC products was carried out on a Waters 2695 coupled with a Waters 2998 Diode array detector, analysis SunFireTMC18 Column (250 mm × 4.6 mm, id 5 μm, Waters Corporation, Milford, MA, USA) and Sigma 1–14 centrifuge (Sigma, St. Louis, MO, USA) with ultramembrane filters (Microcon YM-30; Millipore, Billerica, MA, USA). A semi−preparative HPLC was performed using a 2545 Quaternary Gradient Module pump, a Fraction Collector III, a Waters 2489 UV/Vis detector, and a semi−prep SunFire^TMC₁₈ Column (100 × 19 mm, ID 5 μm, Waters Corporation, Milford, MA, USA). HSCCC was performed on a DE Spectrum HSCCC (Dynamic Extractions, Slough, UK). The kinetics of enzymatic reactions were managed using a High Performance Capillary Electrophoresis apparatus (Beckman Coulter, Krefeld, Germany).

To obtain adequate integration
of MNP in the peptide hydrogels, suitable functionalization was required,
for which we followed a previously published protocol.^{33 (link)} Briefly, we first coated the MNP with PEG using
the microemulsion method of Chatterjee et al.^{54 (link)} Then, we suspended 100 mg PEG-coated MNP in 1.5 mL of an aqueous
basic (pH approx. 10.5) solution of Fmoc-FF (0.5% w/v). The resulting
suspension was sonicated for 10 min and then centrifuged for 5 min
at 10,000 rpm (Sigma 1-14 centrifuge) to obtain MNP with a double
coating of PEG and Fmoc-FF. We removed the supernatant and used the
double-coated MNP to prepare the magnetic hydrogels. A complete characterization
of the physicochemical properties of these particles was reported
previously.^{33 (link)} In particular, the MNP demonstrated
typical soft ferromagnetic behavior with a high saturation magnetization
of 1521 ± 15 kA/m, a low remnant magnetization of 62.3 ±
2.4 kA/m, and negligible effects of PEG coating on the magnetic properties.^{33 (link)}