RGB light sources: Prizmatix UHP-T-LED-460, UHP-T-LED-520 and UHP-T-LED-630. Spatial Light Modulator: Hamamatsu X10468-03. Camera: Basler avA1000-100gc.
X10468 03
Manufactured by Hamamatsu Photonics
The X10468-03 is a high-sensitivity photon-counting module designed for low-light applications. It features a photomultiplier tube (PMT) as the photodetector and provides analog output signals proportional to the detected light intensity. The core function of this product is to convert photons into electrical signals that can be processed and analyzed.
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Lab products found in correlation
4 protocols using x10468 03
1
Multicolor LED-Based Microscopy
2
Photoisomerization of Azo-Polymer Films
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The spin-coated azo-polymer film (mostly the trans isomer) used in this study was formed from a Poly-Orange Tom-1(POT)33 with the absorption band in the wavelength range of 300–550 nm, and its thickness was measured to be ~4 μm. The azo-polymer exhibits a photo-isomerization behavior upon green laser irradiation.
A continuous-wave frequency-doubled Nd:YVO4 laser with a wavelength of 532 nm was used, and its output was converted to be a linearly polarized first-order optical vortex (m = 1) with an annular intensity profile by a polymer spiral phase plate (RPC photonics, VPP-1c) providing an azimuthal 2π phase shift34 (link). To generate a higher-order optical vortex with a topological charge, m, of 6, a computer-generated hologram displayed on a spatial light modulator (Hamamatsu photonics, X10468-03) was used. With this system, the mode conversion efficiency from the Gaussian output to the vortex output was measured to be ~50%. All experiments were performed with an exposure time of 12 seconds at room temperature and atmosphere.
A continuous-wave frequency-doubled Nd:YVO4 laser with a wavelength of 532 nm was used, and its output was converted to be a linearly polarized first-order optical vortex (m = 1) with an annular intensity profile by a polymer spiral phase plate (RPC photonics, VPP-1c) providing an azimuthal 2π phase shift34 (link). To generate a higher-order optical vortex with a topological charge, m, of 6, a computer-generated hologram displayed on a spatial light modulator (Hamamatsu photonics, X10468-03) was used. With this system, the mode conversion efficiency from the Gaussian output to the vortex output was measured to be ~50%. All experiments were performed with an exposure time of 12 seconds at room temperature and atmosphere.
3
Optical Tweezers for Rotating Liquid Crystal Droplets
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In our optical system, the wavefront of the laser beam (YLM-10-CP, IPG Photonics, wavelength of 1064 nm) was modified using a spatial light modulator (SLM, X10468-03, Hamamatsu) to control the spatial intensity pattern at the focal plane. The reflected beam from the SLM was transmitted through a half-wave plate and a quarter-wave plate to control the ellipticity angle, φ, and orientation angle, θ, of the polarization ellipse of the trapping beam. Finally, the polarized light was focused using a 100 × objective lens (Plan Fluor, Nikon, NA1.4). At the focal point, LC droplets were trapped above 20 µm from the bottom of the cell to prevent the wall effect (see Supplemental Information) and were forced to rotate with the trap. The laser power used at the focal plane was 7.5 mW, measured using a power meter (PM16-405, Thorlabs) unless otherwise noted.
4
Optical Trapping with Spatial Light Modulator
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The laser beam (λ = 1064 nm TEM00, IPG YLM-5-1064-LP) was expanded through a telescope to fit the active area of a reflective SLM (Hamamatsu X10468-03: 800 × 600 pixels) and subsequently readjusted to the objective entrance pupil through another telescope. The beam enters an inverted microscope (Nikon Eclipse TE2000-E) through a rear port and a dichroic mirror reflects it up towards the microscope objective (either water immersion Nikon Plan Apo, 60x, NA = 1.2 or oil immersion Nikon CFI Plan Fluor, 100x, NA = 1.3), creating the optical traps at its focal plane. Microchambers were placed onto a piezo electric stage (Piezosystem Jena, TRITOR 102 SG). Lateral optical trapping forces, as well as optical trap intensity, were measured by a direct force-detection instrument (Impetux Optics, LUNAM T-40i). This instrument enables the simultaneous collection of the laser light emerging from the optical traps as well as bright-field illumination, hence allowing sample imaging, which was performed at a different rear port with a CCD camera (QImaging, QICAM).
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