GCaMP6f, GCaMP7f or dLight1.1 were excited by amplitude modulated signals from two light-emitting diodes (465- and 405-nm isosbestic control, DORIC) reflected off dichroic mirrors (6-port minicube, DORIC) and coupled into a single FP optic fiber (400um-core, NA 0.48, DORIC). Sensor signals and isosbestic control emissions were returned through the same optic fiber and acquired using a femtowatt photoreceiver (Newport), digitized at 1017 Hz, and recorded by a real-time signal processor (RZ5P, Tucker Davis Technologies, RRID:SCR_006495). Behavioral and event timestamps were digitized in Synapse software (Tucker Davis Technologies) by TTL inputs from Med-PC, Any-Maze, or manual triggers. Power of light used for imaging (<0.2 mW) is ~2 orders of magnitude less than used for laser stimulation (10 mW), and the distance between the brain sites of stimulation and calcium or DA imaging was >3.5 mm.
6 port minicube
Manufactured by Doric
The 6-port minicube is a compact laboratory equipment designed to provide a controlled environment for various experimental applications. It features six access ports, allowing for the introduction and manipulation of samples or materials within the enclosed space. The device is designed to maintain consistent environmental conditions, such as temperature and atmospheric composition, to facilitate precise and reproducible experiments.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using 6 port minicube
1
Calcium and Dopamine Imaging Protocol
2
Fiber Optic-Based Calcium and Dopamine Imaging
Check if the same lab product or an alternative is used in the 5 most similar protocols
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GCaMP6f, GCaMP7f or dLight1.1 were excited by amplitude modulated signals from two light-emitting diodes (465- and 405-nm isosbestic control, DORIC) reflected off dichroic mirrors (6-port minicube, DORIC) and coupled into a single FP optic fiber (400um-core, NA 0.48, DORIC) through a pigtailed fiber optic rotary joint (400um-core, NA 0.48, DORIC). Sensor signals and isosbestic control emissions were returned through the same optic fiber and acquired using a femtowatt photoreceiver (Newport, DORIC), digitized at 1017 Hz, and recorded by a real-time signal processor (RZ5P, Tucker Davis Technologies, RRID:SCR_006495). Behavioral and event timestamps were digitized in Synapse software (Tucker Davis Technologies) by TTL inputs from Med-PC, Any-Maze, or manual triggers. Power of light used for imaging (<0.2mW) is ~2 orders of magnitude less than used for laser stim (10mW), and the distance between the brain sites of stim and calcium or DA imaging was >3.5 mm.
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