The one-step TUNEL Apoptosis Detection Kit (Green Fluorescence, Beyotime) was used to analyze DNA damage in cardiomyocytes. Treated cardiomyocytes were fixed with 4% paraformaldehyde for 30 min and permeabilized by 0.3% Triton X-100 for 5 min at room temperature. Subsequently, the cells were incubated with TUNEL assay solution for 60 min at 37°C, followed by nuclear labeling with DAPI for 10 min at room temperature (both in the dark). After sealing with an anti-fluorescence quenching sealing solution (Beyotime), the slices were observed under a fluorescence microscope, and the percentage of TUNEL-positive cells was calculated.
One step tunel apoptosis detection kit green fluorescence
Manufactured by Beyotime
Sourced in China
The One-step TUNEL Apoptosis Detection Kit (Green Fluorescence) is a laboratory reagent used to detect and visualize apoptotic cells. It utilizes the TUNEL (Terminal deoxynucleotidyl transferase dUTP Nick End Labeling) method to label DNA strand breaks, which are characteristic of apoptosis. The kit provides a convenient one-step protocol for the detection of apoptotic cells, allowing for the analysis of cellular apoptosis using fluorescence microscopy or flow cytometry.
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2 protocols using one step tunel apoptosis detection kit green fluorescence
1
Quantification of Cardiomyocyte Apoptosis by TUNEL
2
Apoptosis Evaluation in Cervical Cancer
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The apoptosis of cervical cancer cells was evaluated using the one-step TUNEL apoptosis detection kit (green fluorescence) (C1088, Beyotime Biotechnology, Shanghai, China). In brief, cells were fixed with 4% paraformaldehyde for 30 min and resuspended in PBS with 0.3% Triton X-100. A total of 50 μL TUNEL detection solution was added to the sample and incubated for 60 min in the dark at 37 °C. The film was sealed with an anti-fluorescence quenching solution, and cells were observed under a fluorescence microscope.
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