All experiments were performed in accordance with protocols approved by the UC Davis Institutional Animal Care and Use Committee (IACUC protocol #20042). Mice used in this study include CD19cre and XRCC2f/f [50 (link), 51 (link)]. Splenic B cells were isolated using the Dynabeads untouched CD43 mouse B cell isolation kit (Thermo Fisher, 11422D) and cultured as previously described[8 (link)]. Human lymphocytes were obtained from peripheral blood of 3 unrelated volunteers and cultured for 72 h in MF-Chang medium (Irvine Scientific, 91005), supplemented with 10% heat inactivated fetal bovine serum and 3 μg/mL phytohemagglutinin (Remel, Inc., 30852701).
Mf chang medium
Manufactured by Irvine Scientific
MF-Chang medium is a cell culture medium designed for the maintenance and growth of mammalian cells. It provides the necessary nutrients and supplements to support cell viability and proliferation.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using mf chang medium
1
Isolation and Culture of Mouse and Human Lymphocytes
2
Isolation and Culture of Mouse and Human Lymphocytes
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
All experiments were performed in accordance with protocols approved by the UC Davis Institutional Animal Care and Use Committee (IACUC protocol #20042). Mice used in this study include CD19cre and Xrcc2f/f [50 (link), 51 (link)]. Splenic B cells were isolated using the Dynabeads untouched CD43 mouse B cell isolation kit (Thermo Fisher, 11422D) and cultured as previously described [8 (link)]. Human lymphocytes were obtained from peripheral blood of three unrelated volunteers and cultured for 72 h in MF-Chang medium (Irvine Scientific, 91005), supplemented with 10% heat inactivated fetal bovine serum and 3 μg/mL phytohemagglutinin (Remel, Inc., 30852701).
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!