Stock solutions of 1 mM peptides were allowed to fibrillize in water overnight at room temperature, diluted in PBS to 0.3 mM, and applied to 300 mesh copper grids with carbon support film (Quantifoil, Germany). The grids were negatively stained with 2% uranyl acetate, and imaged on a JEM1400 TEM (JEOL) instrument equipped with LaB6 electron gun and digital cameras. Images were viewed and recorded with an Ultrascan 1000 camera (Gatan, Pleasanton, CA).
Carbon support film
Manufactured by Quantifoil
Sourced in Germany
Carbon support film is a thin, electron-transparent substrate used in various electron microscopy applications. It provides a stable platform for mounting and supporting samples during imaging and analysis.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using carbon support film
1
Transmission Electron Microscopy of Peptide Fibrils
2
Peptide Fibrillization and Electron Microscopy
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Stock solutions
of 1 mM peptides were allowed to fibrillize in water overnight at
room temperature, diluted in phosphate-buffered saline (PBS) to 0.2
mM, and applied to 200 mesh copper grids with carbon support film
(Quantifoil). The grids were negatively stained with 2% uranyl acetate,
and imaged on a JEM1400 TEM (JEOL) equipped with LaB6 electron
gun and Ultrascan 1000 camera (Gatan).
of 1 mM peptides were allowed to fibrillize in water overnight at
room temperature, diluted in phosphate-buffered saline (PBS) to 0.2
mM, and applied to 200 mesh copper grids with carbon support film
(Quantifoil). The grids were negatively stained with 2% uranyl acetate,
and imaged on a JEM1400 TEM (JEOL) equipped with LaB6 electron
gun and Ultrascan 1000 camera (Gatan).
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