Lc 6ad series

HSGF₂₅₄ silica gel plates (10–40 μm, Yantai, China) were used for TLC analysis. Silica gel (100–200 mesh, Yantai, China), silica gel H (10–40 μm, Qingdao, China), and Sephadex LH-20 (Pharmacia Co., ltd.) were utilized as column chromatography materials. The Shimadzu LC-6AD series equipped with an SPD-20 spectrophotometer using the Shimadzu PRC-ODS EV0233 column was selected as the preparative HPLC. 1D-¹H NMR, ¹³C NMR, and 2D NMR spectra were conducted on the Bruker Avance-500 NMR spectrometer, and chemical shifts are shown in δ (1 × 10⁻⁶) with TMS as an internal reference and coupling constants were in Hz. ESIMS spectra were measured on Agilent LC/ MSD Trap XCT spectrometer (Waters, USA) and HR-ESI-MS on Q-TOF micro YA019 mass spectrometer (Waters, USA). Optical rotations were calculated on the JASCO P-2000 polarimeter in MeOH at 20 °C.

HSGF₂₅₄ silica gel plates (10–40 μm, Yantai, China) were major used as TLC analysis. Silica gel (100–200 mesh, Yantai, China), silica gel H (10–40 μm, Qingdao, China) and Sephadex LH-20 (Pharmacia Co. Ltd.) were utilized as column chromatography materials. The Shimadzu LC-6AD series equipped with an SPD-20 spectrophotometer using Shimadzu PRC-ODS EV0233 column was selected as the preparative HPLC. 1D and 2D NMR spectra were conducted on the Bruker Avance-500 NMR spectrometer, and chemical shifts are shown in d (ppm) with TMS as an internal reference and coupling constants were in Hz. ESIMS spectra were tested on Agilent LC/MSD Trap XCT spectrometer (Waters, USA) and HR-ESIMS on Q-TOF micro YA019 mass spectrometer (Waters, USA). Optical rotations were tested on the JASCO P-2000 polarimeter in MeOH at 20C. IR spectra were conducted on the Bruker FTIR Vector 22 spectrometer with KBr pellets.