Iscan coreo au scanner

RCC biopsies of patients (24 cases) were obtained from the Department of Pathology, Boston University School of Medicine. Immunohistochemistry (IHC) staining was performed as per the manufacturer’s instruction using the EXPOSE Rabbit specific HRP/DAB detection IHC kit (Abcam, Cambridge, USA). Slides were scanned via iScan Coreo Au scanner (Ventana, AZ, USA). Two surgical pathologists independently evaluated the staining of TMIGD1 and C/EBPβ.

For examination of xenograft tumor, tumors were fixed in 10% neutral buffered formalin, embedded in paraffin and sectioned. One slide (5 um section) per tumor was stained with hematoxylin and eosin (H&E) and another slide was stained for Ki67 (tumor proliferation marker) using a rabbit polyclonal anti-ki67 antibody (1:300 dilution, ab2960, EMD Millipore, Darmstadt, Germany). IHC staining was performed as per the manufacturer’s instruction using the EXPOSE Rabbit specific HRP/DAB detection IHC kit (Abcam, Cambridge, USA). At least three xenografts from each group were included in the analysis. Images were obtained using the iScan Coreo Au scanner (Ventana, AZ, USA).