Penicillin streptomycin solution

U2OS human osteosarcoma cells (American Type Culture Collection) were grown in high glucose Dulbecco’s modified Eagle’s medium (American Type Culture Collection, Manassas, VA) supplemented with 10% fetal bovine serum (Mediatech Inc., Manassas, VA) and 1% penicillin/streptomycin solution (HyClone reagents from GE Healthcare Life Sciences, Pittsburgh, PA). Cells were cultured at 37°C in a humidified atmosphere with 5% CO₂. During cell passaging, cells were checked for the presence of mycoplasma contamination using MycoFluor Mycoplasma detection kit (Thermo Fisher Scientific, Waltham, MA). Cell line authentication was performed at the University of Arizona Genetics Core (Tucson, AZ).

Primary rat mesenchymal stem cells (MM), mouse embryonic fibroblast cells (MEF), murine macrophage cells (Raw 264.7), African green monkey kidney cells (Vero), human renal carcinoma cells (Caki), human cervical adenocarcinoma cells (HeLa), human hepatocellular carcinoma cells (Huh7), human astroglia (SVG p12, ATCC^®CRL-8621™), human lung fibroblasts, and human embryonic kidney cells (293T) were cultured in Dulbecco’s modified Eagle’s medium (DMEM) (Hyclone, GE Healthcare, Chicago, IL) supplemented with 10% fetal bovine serum (FBS) (Sigma, Saint Louis, MO), 1 × penicillin-streptomycin (P/S) solution (Genesee Scientific, San Diego, CA). Human mesenchymal stem cells (MSC) from adipose tissue were cultured in MSC medium complemented with 5% FBS, 1% of MSC growth supplement and 1% of P/S solution (ScienCell, Carlsbad, CA). Human lung carcinoma cells (A549) and human monocytic cells (THP-1) were cultured with RPMI-1640 (Hyclone, GE Healthcare, Chicago, IL) supplemented with 10% FBS, and 1 × penicillin-streptomycin solution. Primary human umbilical vein endothelial cells (HUVEC) were cultured in VascuLife VEGF complete media (Lifeline cell technology, Frederick, MD). All cells were cultivated at 37°C with 5% CO₂.