Tissue sections were imaged on a Nikon E800M microscope using a Nikon DSRi2 camera and Nikon Elements D Version 4 software. Each section was imaged using the same white balance and shading correction settings. Whole image sharpness, contrast, and brightness were uniformly adjusted with Photoshop Elements version 11 software.
Elements d version 4
Manufactured by Nikon
Nikon Elements D Version 4 is a software package designed for advanced image analysis and processing. It provides a comprehensive suite of tools for researchers and scientists working with microscopy and imaging data. The software offers extensive functionalities for image acquisition, processing, analysis, and data management, catering to the needs of a wide range of laboratory applications.
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2 protocols using elements d version 4
1
Microscopic Imaging Optimization Protocol
2
Immunohistochemical Analysis of SEMA4C in Osteosarcoma
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The osteosarcoma tissue microarray was purchased from Biomax (Osteosarcoma: OS804c) containing 40 samples in duplicate. Slides containing 4 μm thick formalin-fixed, paraffin-embedded sections of tumor tissue were deparaffinized and rehydrated. Antigen retrieval was performed in a steamer using 1 mM Tris base EDTA buffer, pH 9.0. After endogenous peroxidase blocking, a protein block was applied. Immunohistochemistry (IHC) for SEMA4C was performed using a rabbit anti-SEMA4C primary antibody (#AF6125, R & D Systems) on an autostainer (Dako). Detection was achieved using the Envision rabbit detection system (Dako) with diaminobenzidine (DAB) as the chromogen. Tissue sections were imaged on a Nikon E800M microscope at 40X magnification using a Nikon DSRi2 camera and Nikon Elements D Version 4 software. Slides were evaluated and scored as previously described [19] .
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