Viable and certified seeds of Glycine max L. cv. SL-525 were surface sterlized in 5% sodium hypochlorite (NaOCl) solution for 10 min. After this, seed priming was done with 1 nM concentration of JA (Sigma chemicals, USA), for 8 h. JA treated and untreated seeds were grown in autoclaved petri dishes lined with Whatman filter paper placed in growth chamber under average day/night temperature of 25°C/16°C and with 80% relative humidity. The 4-day old germinated seedlings were shifted to plastic trays (10 plants per tray) containing peat, perlite and sand (1:1:1,v/v/v) supplemented with 2 mM Ni solution (NiCl2⋅6H2O, Sigma chemicals, USA). Control plants were fed with distilled water only. Each treatment is mean of five replications laid in randomized block design and each replicate includes five plants. The plant samples were collected for analysis after 15 days after treatment (DAT).
Nicl2 6h2o
Manufactured by Merck Group
Sourced in Germany, United States
NiCl2·6H2O is a chemical compound consisting of nickel(II) chloride and six water molecules. It is a green crystalline solid that is soluble in water. NiCl2·6H2O is commonly used as a laboratory reagent and in various industrial applications.
Automatically generated - may contain errors
Lab products found in correlation
Cocl2 6h2o, by Merck Group (17 mentions)
Fecl3 6h2o, by Merck Group (10 mentions)
Cucl2 2h2o, by Merck Group (9 mentions)
Sodium hydroxide (naoh), by Merck Group (8 mentions)
Mncl2 4h2o, by Merck Group (6 mentions)
Sodium chloride (nacl), by Merck Group (4 mentions)
Zncl2, by Merck Group (4 mentions)
Hydrochloric acid (hcl), by Merck Group (4 mentions)
Pb no3 2, by Merck Group (3 mentions)
Urea, by Merck Group (3 mentions)
Crcl3 6h2o, by Merck Group (3 mentions)
Cdcl2, by Merck Group (3 mentions)
Sodium borohydride, by Merck Group (3 mentions)
Fecl2 4h2o, by Merck Group (3 mentions)
Ethanol, by Merck Group (3 mentions)
Hg no3 2 h2o, by Merck Group (2 mentions)
Cd no3 2 4h2o, by Merck Group (2 mentions)
Zn no3 2 6h2o, by Merck Group (2 mentions)
Sncl4 5h2o, by Merck Group (2 mentions)
Methanol, by Merck Group (2 mentions)
59 protocols using nicl2 6h2o
1
Jasmonic Acid Priming of Soybean Seeds
2
Synthesis of Metal-Cichoric Acid Complexes
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The weighed mass of cichoric acid was added to with appropriate volume of NaOH (0.1 M) in a stoichiometric molar ratio 1:1 and stirred at 50 °C by 1 h. Next, the aqueous solution of transition metal chloride was added to the mixture in order to obtain the molar ratio of ligand:transition metal cation 1:2. Then it was left for 2 h at room temperature in a shaker. After several days, precipitates occurred. They were filtered from the solution and washed with deionized water. The precipitates were air-dried at 30 °C. The obtained yield of the synthesis was ~70–85%. Complexes with a ligand:metal ratio (1:2) were obtained.
The chemicals: CuCl2·2H2O, ZnCl2, CoCl2·6H2O, NiCl2·6H2O, NaOH, cichoric acid, tris(hydroksymetylo)aminometan, TRIS–HCl, methanol, DPPH, ABTS, KBr from the Sigma-Aldrich (St. Louis, MO, USA).
The chemicals: CuCl2·2H2O, ZnCl2, CoCl2·6H2O, NiCl2·6H2O, NaOH, cichoric acid, tris(hydroksymetylo)aminometan, TRIS–HCl, methanol, DPPH, ABTS, KBr from the Sigma-Aldrich (St. Louis, MO, USA).
3
Nanocomposite Synthesis for Pollutant Removal
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In the synthesis of nanocomposites, MWCNT (D × L 110–170 nm × 5–9 μm), NiCl2 × 6H2O, ascorbic acid, cetyltrimethylammonium bromide (CTAB) were purchased from Sigma-Aldrich (Schnelldorf, Germany), FeCl3 × 6H2O from Alfa Aesar (Kandel, Germany), FeSO4 × 7H2O from VWR Chemicals (Wien, Austria) and NH3 25% solution from Chemical Company (Iaşi, Romania). Tartrazine was chosen as pollutant in this study and was purchased from Sigma-Aldrich (Schnelldorf, Germany). The pH adjustment was performed with HCl and NaOH that were purchased from Sigma-Aldrich (Schnelldorf, Germany) and VWR Chemicals (Wien, Austria), respectively. Aqueous solutions were prepared using ultrapure water (Direct-Q® 3 UV Water Purification System, Merck, Darmstadt, Germany).
4
Electrochemical Deposition of Metal Nanoparticles
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The RBA technique was applied using a two-electrode system with
Ti foil (99.7%, 1 × 2.5 × 2.5 mm3, Good Fellow)
as the anode and Pt gauze (99.9%, 52 mesh, 2.5 × 2.5 cm2, Sigma-Aldrich) as the cathode. NaCl (0.3 M, Merck) was prepared
using Millipore water and employed as the electrolyte. Chloroplatinic
acid, H2PtCl6 (Sigma-Aldrich), palladium chloride,
PdCl2 (Sigma-Aldrich), and NiCl2·6H2O (Sigma-Aldrich) were employed as precursors for Pt, Pd,
and Ni, respectively. Sodium borohydride, NaBH4 (Merck)
was used as the reducing agent.
Ti foil (99.7%, 1 × 2.5 × 2.5 mm3, Good Fellow)
as the anode and Pt gauze (99.9%, 52 mesh, 2.5 × 2.5 cm2, Sigma-Aldrich) as the cathode. NaCl (0.3 M, Merck) was prepared
using Millipore water and employed as the electrolyte. Chloroplatinic
acid, H2PtCl6 (Sigma-Aldrich), palladium chloride,
PdCl2 (Sigma-Aldrich), and NiCl2·6H2O (Sigma-Aldrich) were employed as precursors for Pt, Pd,
and Ni, respectively. Sodium borohydride, NaBH4 (Merck)
was used as the reducing agent.
5
Metalated PAMAM Dendrimer Autophagy Assay
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2,2':6′,2″-terpyridine-4′-carbaldehyde was purchased from Alfa (Zhengzhou, China). G5 PAMAM dendrimer was purchased from Dendritech (Midland, USA). NaBH3CN was obtained from J&K Scientific (Shanghai, China). Bovine serum albumin (BSA), MnCl2, NiCl2·6H2O and CuCl2·2H2O were purchased from Sigma-Aldrich (St. Louis, USA). FeCl2·4H2O, CoCl2·6H2O and ZnCl2 were purchased from Aladdin (Shanghai, China). All the peptides were purchased from GL Biochem. (Shanghai, China). Anti-LC3B, anti-p62, anti-GAPDH and Goat anti-rabbit Alexa Fluor®680 secondary antibodies were purchased from Abcam (Cambridge, UK). MTT was obtained from Sangon Biotech. (Shanghai, China). Trypan blue was obtained from Yesen (Shanghai, China). TransExcellent™ (TransEx) was obtained from Cenji Biotech. (Shanghai, China).
6
Cytotoxicity and Apoptosis Assay Protocol
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The human monoblastic leukemia cell line THP-1 was obtained from the American Type Culture Collection (Manassas, VA, USA). Cell culture reagents RPMI 1640 medium and fetal bovine serum (FBS) were from Gibco-BRL (Invitrogen Life Technologies, Carlsbad, CA, USA). NiCl2·6H2O, MTT, Hoechst 33342 and propidium iodide (PI) were obtained from Sigma-Aldrich (St. Louis, MO, USA). TRIzol reagent was obtained from Invitrogen Life Technologies. All reagents were of analytical grade.
7
Microwave-Assisted Fabrication of Ta:Fe2O3@Fe2O3 Photoelectrodes
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The well-cut samples (12.5 × 12.5 mm2) were put on the slightly compacted graphite powder (60 mL) as a susceptor in a Pyrex beaker (100 mL) and treated in a home-made microwave oven (2.45 GHz, 1000 W) for 2 min at the full power. After HMA, the Ta:FeOOH@FeOOH was converted into Ta:Fe2O3@Fe2O3 without significant change of morphology.
For cocatalyst modification, a facile, one-step precipitating despostion method was used to prepare amorphous metal (oxy)hydroxide on the surface of nanorods25 . Typically, the fabricated photoelectrode was immersed into a solution containing 5 × 10–3 M FeCl3·6H2O and NiCl2·6H2O (Sigma Aldrich, ≥99%) for different durations (1, 2, and 3 h) at 30 °C. Subsequently, the photoelectrode was rinsed by sufficient deionized water.
For cocatalyst modification, a facile, one-step precipitating despostion method was used to prepare amorphous metal (oxy)hydroxide on the surface of nanorods25 . Typically, the fabricated photoelectrode was immersed into a solution containing 5 × 10–3 M FeCl3·6H2O and NiCl2·6H2O (Sigma Aldrich, ≥99%) for different durations (1, 2, and 3 h) at 30 °C. Subsequently, the photoelectrode was rinsed by sufficient deionized water.
8
Culturing Neuro-2a Cells for Ni-Chloride Treatment
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Mouse neuroblastoma cell lines (Neuro-2a), obtained from the Institute of Biochemistry and Cell Biology (Chinese Academy of Science, Shanghai, China), were cultured in Dulbecco's modified Eagle's medium (DMEM; Invitrogen, Carlsbad, CA, USA) supplemented with 10% heat-inactivated fetal bovine serum (FBS, HyClone, Logan, UT, USA) and 1% v/v penicillin/streptomycin (Sigma-Aldrich, St. Louis, MO, USA) in a 5% CO2-humidified atmosphere at 37°C. A 1 M stock solution of Ni chloride (NiCl2·6H2O, Sigma-Aldrich) was prepared with sterile H2O and diluted to the appropriate concentrations for cell treatment.
9
Preparation of Metal Ion Stock Solutions
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Stock solutions of Co2+, Cr3+, and Ni2+ were prepared fresh, as previously described [37 (link)]. Briefly, CoCl2•6H2O (≥99.5% purity; Fisher Scientific, Waltham, MA), CrCl3•6H2O (≥99.2% purity; Sigma-Aldrich, St Louis, MO) and NiCl2•6H2O (≥99.999% purity; Sigma-Aldrich) were dissolved in cell culture-grade water (Lonza, Walkersville, MD), and the solutions were sterilized by filtration through 0.2-μm pore size cellulose acetate syringe filters (VWR, Mississauga, ON).
10
Buckminsterfullerene Oxidation and Metal Sensitivity
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Buckminsterfullerene (C60, TERM USA, Fort Bragg, CA, USA), sulfuric acid (H2SO4, 99.9%, Sigma Aldrich, Milano, Italy), sodium nitrate (NaNO3, Sigma Aldrich), sodium hydroxide (NaOH, Carlo Erba, Milano, Italy), potassium permanganate (KMnO4, Sigma Aldrich), and hydrogen peroxide (H2O2, Sigma Aldrich) were used as purchased without further purification. Metal salts used for studying the system sensitivity, purchased from Sigma Aldrich, are NiCl2 6H2O, NaCl, CoCl2 6H2O, CuCl2 6H2O, Pb(NO3)2 5H2O, Hg(NO3)2 H2O, NaAsO2, FeSO4 7H2O). The metal salts solutions were prepared at the concentration of 1000 μM. For this work, deionized water obtained from a Milli-Q water purification system (Millipore, Burlington, MA, USA) was used.
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