Chloroauric acid (HAuCl4·3H2O), and the other pure chemicals and biochemicals were purchased from Sigma-Aldrich, potato dextrose broth was obtained from Lab M (Bury, United Kingdom).
Chloroauric acid haucl4 3h2o
Manufactured by Merck Group
Sourced in United States, India, Canada
Chloroauric acid (HAuCl4·3H2O) is a chemical compound composed of gold, chlorine, and hydrogen. It is a yellow crystalline solid that is soluble in water. Chloroauric acid is commonly used as a precursor in the synthesis of gold nanoparticles and other gold-containing materials.
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Lab products found in correlation
Bovine serum albumin (bsa), by Merck Group (3 mentions)
Silver nitrate agno3, by Merck Group (3 mentions)
Milli q, by Merck Group (2 mentions)
Nitrocellulose nc membrane, by Sartorius (2 mentions)
Sodium citrate tribasic dehydrate hoc coona ch2coona 2 2h2o, by Merck Group (2 mentions)
Sepharose beads, by Merck Group (1 mentions)
F 4600 spectrofluorophotometer, by Hitachi (1 mentions)
Uh5300 spectrophotometer, by Hitachi (1 mentions)
S 4800, by Hitachi (1 mentions)
α tubulin, by Cell Signaling Technology (1 mentions)
Mmp 2, by Cell Signaling Technology (1 mentions)
Mmp 9, by Cell Signaling Technology (1 mentions)
Methyl thiazolyl tetrazolium (mtt), by Merck Group (1 mentions)
Hoc coona ch2coona 2 2h2o, by Merck Group (1 mentions)
Tetraethyl orthosilicate, by Merck Group (1 mentions)
Sodium hydroxide (naoh), by Avantor (1 mentions)
Trisodium citrate na3c6h5o7 2h2o, by Merck Group (1 mentions)
Agno3, by Merck Group (1 mentions)
Ascorbic acid, by Sinopharm (1 mentions)
Tetraethyl orthosilicate teos, by Merck Group (1 mentions)
28 protocols using chloroauric acid haucl4 3h2o
1
Gold Nanoparticle Synthesis Protocol
2
Gold Nanoparticle Stabilization Protocol
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Chloroauric acid (HAuCl4·3H2O) was obtained from Aldrich. Iodine-125 (1 mCi; specific activity 17 Ci/mg; concentration 100 mCi/ml) was purchased from Perkins-Elmer. Magnetic beads (Protein A Mag Sepharose) were purchased from GE Healthcare. Sepharose beads and all proteins used for gold stabilisations and all other chemicals were obtained from Sigma.
3
Bilirubin-Stabilized Gold Nanoclusters
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Bovine serum albumin (BSA) and chloroauric acid (HAuCl4.3H2O) were purchased from Sigma-Aldrich and Shanghai Reagent (Shanghai, China), respectively. Glutaraldehyde, bilirubin, and all other reagents used in the experiment were obtained from Aladdin Reagent (Shanghai, China). Bilirubin (1 mg) was first dissolved with 0.1 M NaOH (0.1 mL) and then diluted with 50 mM phosphate-buffered solution (PBS) (pH 7.4) to 10 mL to obtain a stock solution (171 μM). Chemicals and solvents were all of analytical reagent grade unless otherwise stated. Ultrapure water (18.2 MΩ, Milli-Q, Millipore) was used throughout the experiment.
Fluorescence spectra and UV-vis absorption spectra were measured with a Hitachi F-4600 spectrofluorophotometer and a Hitachi UH-5300 spectrophotometer, respectively. Scanning electron microscopy (SEM) images were obtained from a field emission SEM (Hitachi S-4800). The morphology and size of the gold nanoclusters were characterized by a JEOL 2100 high-resolution transmission electron microscope (HRTEM) operating at an accelerating voltage of 200 kV.
Fluorescence spectra and UV-vis absorption spectra were measured with a Hitachi F-4600 spectrofluorophotometer and a Hitachi UH-5300 spectrophotometer, respectively. Scanning electron microscopy (SEM) images were obtained from a field emission SEM (Hitachi S-4800). The morphology and size of the gold nanoclusters were characterized by a JEOL 2100 high-resolution transmission electron microscope (HRTEM) operating at an accelerating voltage of 200 kV.
4
Cellular Assay for Evaluating Anti-Cancer Activity
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Phorbol-2-myristate-13-acetate (PMA), 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazoliumbromide (MTT), chloroauric acid (HAuCl4·3H2O), and other components were obtained from Sigma-Aldrich (Saint Louis, MO, USA). The antibodies against α-tubulin, MMP-9, MMP-2, PLD1, and PLD2 were purchased from Cell Signaling Technology, Inc. (Beverly, MA, USA). All other chemicals were of analytical grade.
5
Silver Nitrate and Gold Synthesis
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Silver nitrate (AgNO3; Merck, India) and chloroauric acid (HAuCl4·3H2O; Sigma Aldrich, USA) were used in this study. All the microbiological media and chemicals were obtained from HiMedia Laboratories, India. Ultrapure Milli Q water was used throughout the study.
6
Synthesis of Spherical Gold Nanoparticles
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Spherical GNPs were prepared via a modified citrate reduction method [30 (link)]. First, 300 μL of 1% chloroauric acid (HAuCl4·3H2O) (Sigma-Aldrich, Oakville, ON, Canada) was added to 30 mL of double–distilled water and brought to boil on a hot plate while stirring. The amount of the reducing agent added varied depending on the size of the NPs.
For example, through optimization studies performed it was determined that the addition of 1 mL and 300 μL of 1% sodium citrate tribasic dehydrate (HOC (COONa)(CH2COONa)2·2H2O) (Sigma-Aldrich) synthesized 15 nm and 50 nm GNPs, respectively. After the color of the solution changed from dark blue to bright red, the solution was left to boil for another ten minutes while being stirred. Finally, the GNP solution was brought to room temperature while being stirred.
For example, through optimization studies performed it was determined that the addition of 1 mL and 300 μL of 1% sodium citrate tribasic dehydrate (HOC (COONa)(CH2COONa)2·2H2O) (Sigma-Aldrich) synthesized 15 nm and 50 nm GNPs, respectively. After the color of the solution changed from dark blue to bright red, the solution was left to boil for another ten minutes while being stirred. Finally, the GNP solution was brought to room temperature while being stirred.
7
Synthesis of Gold Nanoparticles
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All materials were used without further purification; chloroauric acid (HAuCl4·3H2O, 99.995%, Sigma-Aldrich), sodium hydroxide (NaOH, 98.15%, J. T. Baker), ammonium hydroxide (NH4OH, 28–30%, Cicarelli), ethanol (99.5%, Cicarelli), tetraethyl orthosilicate (TEOS, 98%, Sigma-Aldrich) and acetone (99.5, Sintorgan). Deionized purified water (Milli Q system) was used to prepare solutions (resistivity of 18.2 MΩ cm). HAuCl4 stock solutions were kept in darkness to prevent any photochemical reaction.
8
Fabric-Assisted Gold Nanoparticle Synthesis
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Scoured and bleached plain weave polyester (100%, 109 g m−2) and mill-scoured, bleached knitted cotton (200 g m−2) fabrics were used.
Sera® Binder M-CPB liquid (acrylate-based copolymer, anionic, Clariant) and Sera® print M-CPK thickener 160 EG liquid (synthetic thickening agent based on ammonium polyacrylate, Clariant) were used.
Chloroauric acid (HAuCl4·3H2O, Sigma-Aldrich) was purchased from Skyspring Nanomaterials, Inc. USA.
All other chemicals used during this study such as AgNO3 (Sigma) and tri-sodium citrate Na3C6H5O7·2H2O (99.5%) were of commercial grade.
Sera® Binder M-CPB liquid (acrylate-based copolymer, anionic, Clariant) and Sera® print M-CPK thickener 160 EG liquid (synthetic thickening agent based on ammonium polyacrylate, Clariant) were used.
Chloroauric acid (HAuCl4·3H2O, Sigma-Aldrich) was purchased from Skyspring Nanomaterials, Inc. USA.
All other chemicals used during this study such as AgNO3 (Sigma) and tri-sodium citrate Na3C6H5O7·2H2O (99.5%) were of commercial grade.
9
Synthesis of Metal Nanoparticles
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Chloroauric acid (HAuCl4·3H2O) and tetraethyl orthosilicate (TEOS) were purchased from Sigma-Aldrich. Ascorbic acid (AA) and silver nitrate (AgNO3) were purchased from Sinopharm Chemical Reagent Ltd. (Shanghai, China). Cetyltrimethyl ammonium bromide (CTAB) and sodium borohydride (NaBH4) were purchased from Aladdin Reagent Co. Ltd. (Shanghai, China). Potassium permanganate (KMnO4) was purchased from Lingfeng chemical reagent CO. Ltd. (Shanghai, China).
10
Synthesis of Gold Nanoparticles
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All chemical reagents were commercially available and used without further purification. Chloroauric acid (HAuCl4·3H2O), glycine, ninhydrin hydrate, d -glucose, sodium acetate, and sodium bicarbonate were purchased from Sigma-Aldrich. BSA, 3-aminophenylboronic acid (PBA-NH2), 1-ethyl-(3-dimethylaminopropyl)-carbodiimide hydrochloride (EDC), N-hydroxysuccinimide (NHS), morpholine ethanesulfonic acid (MES), and glutaraldehyde solution were obtained from Aladdin. Bovine insulin was purchased from Shanghai Yuanye Bio-Technology. Ultrapure water (Milli-Q) with a resistivity of 18.2 MΩ was used as a general solvent throughout the study.
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