Anti mouse igg conjugated peroxidase antibodies

Manufactured by Cytiva

Anti-mouse IgG conjugated peroxidase antibodies are a type of immunological detection reagent. They are designed to bind to mouse immunoglobulin G (IgG) molecules and are conjugated to the enzyme peroxidase. This allows for the detection and visualization of target mouse IgG proteins in various experimental procedures.

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Lab products found in correlation

Tris glycine gel, by Thermo Fisher Scientific (2 mentions) C digit blot scanner, by LI COR (2 mentions) Ie1 2, by Merck Group (2 mentions) β actin, by Fortis Life Sciences (2 mentions)

Close spelling variants of this product

Horseradish peroxidase-conjugated anti-rabbit or anti-mouse IgG antibodies (2 citations) Horseradish peroxidase-conjugated anti mouse IgG antibodies (2 citations) Anti-rabbit or mouse IgG antibodies conjugated to horseradish peroxidase (2 citations) Secondary horseradish peroxidase-conjugated anti-mouse IgG antibodies (2 citations)

2 protocols using anti mouse igg conjugated peroxidase antibodies

Cytomegalovirus Protein Expression Kinetics

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U373 cells were either infected with TB40E-pp150-GFP at a MOI=5 or left uninfected. At 3, 6, 9, 12, or 15 days post-infection, cells were harvested and lysed in 3X Laemmli Protein Sample Buffer. Samples were sonicated on ice twice for 10 s each and then boiled for 5 min prior to loading onto a 4–20% Tris-glycine gel (Invitrogen). Samples were probed for IE1/2 (Chemicon), UL44 (kind gift from John Shanley), pp65 (Virusys Corporation), and β-actin (Bethyl Laboratories). Anti-mouse IgG conjugated peroxidase antibodies (Cytiva) were used as secondary antibodies for the detection of anti-IE1/IE2, UL44, and pp65 primary antibodies. The β-actin antibody is pre-conjugated to peroxidase. Chemiluminescence signals were detected using a C-Digit blot scanner (LiCor).

Kalavacharla A., Koon C.R., Freeman M.R, & Miller W.E. (2023). Atypical patterns of human cytomegalovirus infection and spread in U373 glioblastoma cells. Virus Research, 330, 199112.

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Cytomegalovirus Protein Expression Kinetics

Check if the same lab product or an alternative is used in the 5 most similar protocols

U373 cells were either infected with TB40E-pp150-GFP at a MOI=5 or left uninfected. At 3, 6, 9, 12, or 15 days post-infection, cells were harvested and lysed in 3X Laemmli Protein Sample Buffer. Samples were sonicated on ice twice for 10 seconds each and then boiled for 5 minutes prior to loading onto a 4-20% Tris-glycine gel (Invitrogen). Samples were probed for IE1/2 (Chemicon), UL44 (kind gift from John Shanley), pp65 (Virusys Corporation), and β-actin (Bethyl Laboratories). Anti-mouse IgG conjugated peroxidase antibodies (Cytiva) were used as secondary antibodies for the detection of anti-IE1/IE2, UL44, and pp65 primary antibodies. The β-actin antibody is pre-conjugated to peroxidase. Chemiluminescence signals were detected using a C-Digit blot scanner (LiCor).

Gram H., Marconi L.A., Barbas CF 3.r.d., Collet T.A., Lerner R.A, & Kang A.S. (1992). In vitro selection and affinity maturation of antibodies from a naive combinatorial immunoglobulin library.. Proceedings of the National Academy of Sciences of the United States of America, 89(8), 3576-3580.

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