Mda mb 231 cell line ko 231

Human kidney embryo cell line HEK293 (WT-HEK), breast cancer cell line MDA-MB-231 (WT-231), AU565, and T47D were obtained from ATCC^® (Manassas, VA, USA). The murine TRPM7 gene (GenBankTM accession number AF376052) overexpressing HEK293 clone (HEK-M7) was a gift from Dr. Loren Runnels, Robert Wood Johnson Medical School (Piscataway, NJ, USA) [32 (link)]. TRPM7 knock-out MDA-MB-231 cell line (KO-231) was obtained commercially from GenScript (Piscataway, NJ, USA) through GenCRISPRTM Technology and the knockout confirmed by genetic sequencing. The genetic sequencing shows a frameshift mutation at the TRPM7 gene (evidence can be provided upon reasonable request). HEK293 and MDA-MB-231 were cultured in Dulbecco’s Modified Eagle Media (DMEM) with 10% Fetal bovine serum (FBS), 10 mM piperazineethanesulfonic acid (HEPES), and 2% pen/strep. AU565 and T47D were cultured in Roswell Park Memorial Institute (RPMI)-1640 with 10% FBS, 10 mM, HEPES, and 2% pen/strep. All cell lines were cultured at 37 °C in a 5% CO₂ humidified incubator and the medium was exchanged once per two days.

HEK293 human kidney embryo cell line (WT-HEK), breast cancer cell lines MDA-MB-231 (WT-231), AU565, T47D, MDA-MB-468, MCF-7, BT474, and BT-20 were purchased from ATCC^® (Manassas, VA, USA). HEK-M7, HEK293 clone with the tetracycline-inducible murine TRPM7 gene (GenBankTM accession number AF376052) was a gift from Dr. Loren Runnels, Robert Wood Johnson Medical School (Piscataway, NJ, USA) [31 (link)]. TRPM7 knock-out MDA-MB-231 cell line (KO-231) was obtained from GenScript (Piscataway, NJ, USA) through GenCRISPRTM Technology [22 (link)].