The antibodies used for Western blot analysis included (a) anti‐ LOX (no. 31238), anti‐β‐arrestin1 (no. 32099), anti‐elastin (no. 21610), anti‐Col1 (no. 34710), and anti‐GAPDH (no. 82451), purchased from Abcam; (b) anti‐ ERK1/2 (no. 4695), and anti‐p‐ERK1/2 (no. 4370), purchased from Cell Signaling Technology; and (c) anti ‐STAT3 (sc‐482), anti‐STAT3‐pS727 (no. sc‐800R) and anti‐STAT3‐pY705 (sc‐7993) were purchased from Santa Cruz Biotechnology Inc. The secondary antibodies, including donkey anti‐rabbit IgG–horseradish peroxidase (sc‐2313), and goat anti‐mouse IgG–horseradish peroxidase (sc‐2005) were purchased from Santa Cruz Biotechnology Inc.
Anti lox
Anti-LOX is a laboratory reagent used to inhibit the activity of the enzyme Lysyl Oxidase (LOX). LOX is involved in the crosslinking of collagen and elastin, which are important structural components of the extracellular matrix. Anti-LOX can be used to study the role of LOX in various biological processes.
Lab products found in correlation
7 protocols using anti lox
Modulation of Kidney Cell Signaling
The antibodies used for Western blot analysis included (a) anti‐ LOX (no. 31238), anti‐β‐arrestin1 (no. 32099), anti‐elastin (no. 21610), anti‐Col1 (no. 34710), and anti‐GAPDH (no. 82451), purchased from Abcam; (b) anti‐ ERK1/2 (no. 4695), and anti‐p‐ERK1/2 (no. 4370), purchased from Cell Signaling Technology; and (c) anti ‐STAT3 (sc‐482), anti‐STAT3‐pS727 (no. sc‐800R) and anti‐STAT3‐pY705 (sc‐7993) were purchased from Santa Cruz Biotechnology Inc. The secondary antibodies, including donkey anti‐rabbit IgG–horseradish peroxidase (sc‐2313), and goat anti‐mouse IgG–horseradish peroxidase (sc‐2005) were purchased from Santa Cruz Biotechnology Inc.
Cell Signaling Modulators in ECM Interactions
Immunofluorescence Labeling of Cellular Proteins
Immunocytochemistry Protocol for Quantifying Mitotic Lox Levels
Immunohistochemical Analysis of LOX and HIF-α
Protein Extraction and Western Blot Analysis
Immunohistochemistry and Immunoblotting of Aortic Lysates
Frozen aortas were pulverized on dry ice before lysis in 25 mM Tris (pH 7.5), 100 mM NaCl, 5 mM EDTA, and 1% Triton X-100 containing Protease Inhibitor Cocktail (MilliporeSigma). Samples were centrifuged for 30 min at 15,000 g, and pellets were solubilized in Laemmli buffer. Proteins of both lysates were separated by SDS-PAGE and electrotransfered to PVDF membranes for immunodetection of LOX, LOXL2 (Abnova), fibronectin (Merck), and actin (Abcam).
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