Rabbit anti cyclinb1

Cellular lysates and western blotting assays were performed as previously depicted [20 (link)–22 (link)]. The following antibodies were used: mouse anti-β-actin (Sigma, St. Louis, MO, USA), rabbit anti-Mxi1-0 (Invitrogen, Carlsbad, CA), rabbit anti-ERK1/2, anti-phospho-ERK1/2 (Thr202/Tyr204), anti-phospho-p38, anti-p38, anti-phospho-JNK, anti-JNK, anti- phospho-Cdk1(Tyr15) and anti-phospho-Chk1(Ser345) (Cell Signaling Technology, Boston, MA, USA), rabbit anti-CyclinB1(Proteintech,Wuhan, China). Antiserum against Mxi1 and Mxi1-0 were raised in rabbits by injection of Keyhole Limpet Hemocyanin (KLH)–conjugated, synthetic peptides (CEAAEFLERRE for Mxi1, GKRGRPRKEARCE for Mxi1-0), corresponding to amino acids 13–23 of Mxi1 and amino acids 2–14 of Mxi1-0, respectively. Antibodies were affinity-purified by Invitrogen (Carlsbad, CA, USA).

Rabbit anti-ACAT2 (1:1000) (ab131215) and rabbit anti-P21 (1:1000) (ab109520) were purchased from Abcam. Rabbit anti-SETD7 antibody(1:1000) (24840-1-AP), rabbit anti-P16-INK4A(1:1000) (10883-1-AP), rabbit anti-P27 (1:1000)(25614-1-AP), rabbit anti-Cyclin B1 (1:1000)(55004-1-AP), rabbit anti-Cyclin E1(1:1000)(11554-1-AP), mouse anti-Cyclin D1(1:1000)(60186-1-Ig), rabbit anti-MCM2(1:1000)(10513-1-AP), rabbit anti-Cortactin (1:400)(11381-1-AP) and rabbit anti-SMA (1:1000)(14395-1-AP), mouse anti-Vimentin(1:4000)(60330-1-Ig) were purchased from Proteintech. Rabbit anti-Snail2 (1:1000) (121235) was purchased from Brickell Biotech, Inc. Antibodies against YAP1(1:1000) (A1002), TAZ (1:1000) (A23034), TEAD1(1:1000) (A5218) were purchased from AB clonal. Rabbit anti-vinculin (1:1000) (E1E9V), rabbit anti-flag antibody (1:1000) (2272S), rabbit anti-myc antibody (1:1000) (14793S), rabbit anti-p53 (7F5) (1:1000)(2527S), rabbit anti-E-Cadherin (24E10) (1:1000)(3195S), rabbit anti-N-Cadherin (D4R1H) XP®(1:1000)(13116S) and mouse anti-ubiquitin (1:1000) (#3936) were purchased from Cell Signalling Technology.
MG132 (HY-13259), Cycloheximide (CHX) (HY-12320) and Cell Counting Kit-8 (CCK-8, HY-K0301) were purchased from MedChemExpress (Shanghai, NJ, USA).

Primary antibody rabbit anti-MAD2B (1:300 dilution; Rockland Immunochemicals Inc., Gilbertsville, PA, USA), mouse anti-NeuN (1:50 dilution; Millipore Corporation, Billerica, MA, USA), rabbit anti-cyclin B1 (1:50 dilution; Proteintech Group, Wuhan, Hubei, China) was used in this study. After incubating the primary antibodies overnight at 4°C, the slides were incubated with different fluorescein-labelled secondary antibodies. Finally, the slides were mounted and subjected to examinations by using a confocal laser scanning microscope (Fluoview FV1000; Olympus, Tokyo, Japan).