C57BL/6 mice (H-2b) were originally obtained from Charles River Laboratories and further bred in the animal facilities of the University of Konstanz. Mice were kept under pathogen-free conditions with a light-dark cycle of 12 h and used at 8–12 weeks of age, at a weight of 20–25 g, in accordance with the rules of the authority of Regierungspräsidium Freiburg (G-18/10). The number of mice for each set of experiments was determined using G*Power 3.0.10 (comparison of two independent groups, effect size d: 0.79, α err prob: 0.05 [25 (link)]. Food and water were provided ad libitum. Mice were infected with 200 pfu LCMV-WE i.v. CB-5083 was dissolved in 0.5% methyl cellulose in water and administered by oral gavage (50 mg/kg) [19 (link), 20 (link)]. Control groups (vehicle) were treated with 0.5% methyl cellulose in water. Mice were randomly allocated to experimental groups and no blinding method was used. There was no animal exclusion criteria. Animal experiments were conducted in full accordance with the ARRIVE guidelines.
C57bl 6 h 2b mice
Manufactured by Charles River Laboratories
Sourced in China, Germany
C57BL/6 (H-2b) mice are an inbred strain of laboratory mice commonly used in research. These mice have a black coat color and are a widely used model for studying various diseases and biological processes.
Automatically generated - may contain errors
Lab products found in correlation
Tumor necrosis factor (tnf), by Thermo Fisher Scientific (1 mentions)
Streptozotocin (stz), by Merck Group (1 mentions)
Balb c h 2d mice, by Charles River Laboratories (1 mentions)
C3h h 2k, by Charles River Laboratories (1 mentions)
C57bl 6 foxp3tm1flv j, by Jackson ImmunoResearch (1 mentions)
C57bl 6 tg tcratcrb 425cbn j, by Jackson ImmunoResearch (1 mentions)
22 protocols using c57bl 6 h 2b mice
1
LCMV infection in C57BL/6 mice
2
Isolation of Murine Peritoneal Exudate Cells
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C57BL/6 mice (H2b, aged 8–20 weeks) were obtained from Charles River (Sulzfeld, Germany) and housed under SPF conditions in individually ventilated cages (IVCs) at the animal facility of the DKFZ. C57BL/6 mice were injected intraperitoneally (i.p.) with 1 ml of 3% (w/v) thioglycolate containing medium (Applichem, Darmstadt, Germany; Cat. No. A3869) using a 27 G needle. Four days later, mice were sacrificed by gradual CO2 exposition and PECs were isolated upon lavage of the peritoneum with 8 ml ice cold PBS. This procedure yielded approximately 1.5 x 107 PECs per mouse.
3
MOG-induced EAE mouse model
4
Studying Autoimmune Skin Conditions
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C57BL/6 mice (H-2b) were originally obtained from Charles River Laboratories. Card14ΔE138+/-mice were originally described in (9 (link)). Naïve C57BL/6 mice were used as negative controls for Card14ΔE138+/-mice. IL-17A-GFP (C57BL/6-Il17atm1Bcgen/J; stock #018472 (23 )) mice were purchased from The Jackson Laboratories. The animal study was reviewed and approved by Regierungspräsidium Freiburg (G-20/20).
5
Generation and Characterization of ApcMin/+ LMP7-/- Mice
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C57BL/6 mice (H-2b) were obtained from Charles River (Sulzfeld, Germany). LMP7-/- mice were kindly provided by Dr. John J. Monaco (Department of Molecular Genetics, Cincinnati Medical Center, OH). C57Bl/6J-ApcMin/+[CD44] mice on a pure C57BL/6 background were obtained from Prof. Jan Paul Medema (University of Amsterdam, The Nederlands). LMP7-/- mice were bred with ApcMin/+ mice to obtain ApcMin/+ LMP7-/- mice. Animals were housed in an air-conditioned, accredited animal facility on a 12 hour light/dark cycle and fed standard, autoclaved laboratory animal diet and water ad libitum. Genotypes of mice were verified via PCR using genomic DNA extracted from tail biopsies to determine knockout and Apc heterozygous state. Investigations have been conducted in accordance with ethical standards, the Declaration of Helsinki, German and EU guidelines and have been approved by the animal experimentation review board of Regierungspräsidium Freiburg.
6
MOG-induced EAE model with hNSC transplant
7
C57BL/6 Mouse Genetic Manipulation
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C57BL/6 mice (H-2b) were originally purchased from Charles River, Germany. MECL-158 (link), LMP259 (link), and LMP7 gene-targeted mice60 (link) were provided by John Monaco (University of Cincinnati, Cincinnati, OH). Mice were kept in a specific pathogen-free facility and used at 8–10 weeks of age. Animal experiments were approved by the Review Board of Governmental Presidium Freiburg of the State of Baden-Württemberg. All methods were carried out in accordance with the approved guidelines.
8
hNSC Transplant in MOG-Induced EAE
9
Murine Macrophage and Fibroblast Isolation
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C57BL/6 mice (H-2b) were originally purchased from Charles River. LMP2 (Van Kaer et al., 1994) , LMP7 (Fehling et al., 1994) , and MECL-1 (Basler et al., 2006) gene-targeted mice were provided by J. Monaco (Cincinnati, OH, USA). LMP7 -/-/MECL-1 -/-double deficient mice (L7 M -/-) were generated by crossing the F1 generation of LMP7 -/-x MECL-1 -/-mice. Mice were kept in a specific pathogen-free facility and used at 8-12 weeks of age. Animal experiments were approved by the review board of Regierungspr äsidium Freiburg. Peritoneal macrophages and primary mouse embryonic fibroblasts (MEFs) were cultured in DMEM with GlutaMAX supplemented with 10% FCS and 100 U/ml penicillin/streptomycin. Media and supplements were purchased from Invitrogen-Life Technologies. Recombinant murine IFN-␥ and TNF-␣ was purchased from Peprotech and used at 200 U/ml and 100 U/ml, respectively.
10
C57BL/6 Mouse Immunization Protocol
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Six-week-old female C57BL/6 mice (H-2b) were purchased from Vital River Laboratory Animal Technology Co., Ltd. (Beijing, China). All animals were immunized at the animal biosafety facilities and all procedures were approved by the institutional animal ethics approval committee of Yangzhou University. Animals received free access to water and commercial mouse chow throughout the study. The challenge experiments were conducted in an ABSL-3 lab at Wuhan University.
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