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2 protocols using anti ki67

1

Ki67 Expression Analysis by Flow Cytometry

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For the detection of Ki67 expression, 1 × 105 SM were fixed and stained for 60 min at 20°C with anti-Ki67 (Miltenyi), then analyzed by flow cytometer.
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2

Multi-Phenotypic Flow Cytometry Analysis

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Flow Cytometry was conducted using a MACSQuant® Analyzer 10 (Miltenyi Biotec), and the following antibodies: CD43-FITC(1G10), CD43-PerCP(1G10), CD235a-PE(HIR2), CD45-BV421(H130), CD45-PerCP(Hl30), CD94-Fitc(HP-3D9), CD38-FITC (HIT2), CD16APC(3G8), CD7-PE(MT701), CD16-FITC (3GB), CD42b-PE(HIP1), CD107a-FITC (H4A3) perforin-FITC (δG9), CD34 FITC (581), CD66BV421 (G10F5), CD16 PE (3G8), and CD14 PerCP (M5E2) from BD Pharmingen; CD41a-APC(REA386), CD45-APC(REA747), CD11b APC(REA713), CD201-PE (REA337), and anti-Ki67 (REA183) from Miltenyi Biotech, and CD56-PerCP(5.1H11), CD56-APC(5.1.H11), and CD45RA-APC (H1100) from BioLegend. All expansions were performed using ultra-low attachment surface, tissue culture plates (CORNING).
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