N n series

The β-1, 3 glucanase enzyme was determined following the method of Pan and co-workers (1991). Fresh leaf samples 0.1 g was homogenized in 0.05 M sodium acetate buffer 2.0 ml (pH 5.0) and the sample was centrifuged at 13,000 g at 4°C for 15 min. The reaction was started by mixing supernatant 0.25 ml, 1 M sodium acetate buffer 0.3 ml (pH 5.3) and laminarin 0.5 ml and after solution was incubated at 40°C for 60 min. The reaction is stopped by adding 3, 5dinitrosalicylic acid 0.375 ml. The nal-colored solution was diluted with distilled water and absorbance was measured at 500 nm. The β-1, 3 glucanase enzyme activity was de ned as µg glucose released min -1 g -1 fresh weight. High performance liquid chromatography (HPLC) analysis of phenylpropanoid derivatives Take 1 g of fresh leaf sample harvested 0 h, 24 h, 48 h, and 72 h after inoculation of the pathogen and was homogenized with 10 ml of 50% methanol and centrifuged for 15 min at 13,000 g. After centrifugation solvent was removed under reduced pressure on rotary evaporator (Eyela N-N series, Japan). The residue was dissolved with HPLC grade methanol. The compounds separation was done using the protocol of Singh et al., (2009) . The solvent ow rate was 1.0 mL min -1 and phenolics were detected using UV detector SPD-10A.Their identi cation was done by comparing the retention times with those from authentic standards at 254 nm.