Mimics nc

Manufactured by Thermo Fisher Scientific

Sourced in United States

The Mimics-NC is a laboratory instrument designed for nucleic acid amplification and detection. It provides a platform for conducting various molecular biology techniques, including real-time PCR, digital PCR, and other nucleic acid-based assays. The core function of the Mimics-NC is to enable efficient and accurate nucleic acid analysis in a controlled laboratory environment.

Automatically generated - may contain errors

Lab products found in correlation

Lipofectamine 2000, by Thermo Fisher Scientific (6 mentions) Inhibitor nc, by Thermo Fisher Scientific (5 mentions) Mimics nc, by GenePharma (2 mentions) Lipofectamine 3000 reagent, by Thermo Fisher Scientific (2 mentions) Pcdna3.1 vector, by Thermo Fisher Scientific (1 mentions) Inhibitor nc, by GenePharma (1 mentions) Mir 29a mimic, by GenePharma (1 mentions) Polybrene, by Merck Group (1 mentions) Mir 29a inhibitor, by GenePharma (1 mentions) Inhibitor nc, by RiboBio (1 mentions) Shrna nc, by Thermo Fisher Scientific (1 mentions) Mir nc, by RiboBio (1 mentions) Adeno x expression system, by Takara Bio (1 mentions) Mir 27b mimics, by Thermo Fisher Scientific (1 mentions) Pcdna3.1 vector, by RiboBio (1 mentions) Mir 103a 3p inhibitor, by GenePharma (1 mentions) Mir 103a 3p inhibitor, by Thermo Fisher Scientific (1 mentions) Si fbxw7, by RiboBio (1 mentions) Si scramble, by RiboBio (1 mentions) Mir 103a 3p mimics, by GenePharma (1 mentions)

Close spelling variants of this product

NC mimic (79 citations) MiR-NC mimic (21 citations) MiRNA mimic NC (3 citations)

9 protocols using mimics nc

NEAT1 Silencing and BCL2L11 Overexpression

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Synthesized NEAT1 small interference RNA (si-NEAT1) was cloned into the vector (pAdTrack-CMV, GenePharma, Shanghai, China) and scrambled siRNA (si-nc) was cloned into the vector as a negative control. HEK-293 cells were used to pack lentivirus by transfection with vectors containing si-NEAT1 or si-nc. After 72 h of transfection, the cells were infected with virus particles using Polybrene (Sigma, St Louis, MO).
To obtain BCL2L11 overexpression plasmid, BCL2L11 was first amplified from cDNA using PCR and then inverted into pcDNA3.1 vector (Invitrogen, Carlsbad, CA) with the name of pcDNA3.1-BCL2L11.
miR-29a inhibitor, miR-29a mimics, inhibitor nc, and mimics nc were purchased from GenePharma (Shanghai, China).
After vector construction, the treated cells were transfected with si-nc, si-NEAT1, miR-29a inhibitor, inhibitor nc, miR-29a mimics, mimics nc, pcDNA3.1, or pcDNA3.1-BCL2L11 using Lipofectamine 2000 (Invitrogen, Carlsbad, CA).

Ban Y, & Cui C. (2020). Silencing of Long Non-Coding RNA (lncRNA) Nuclear Paraspeckle Assembly Transcript 1 (NEAT1) Protects PC-12 Cells from LPS-Induced Injury via Targeting miR-29a. Medical Science Monitor : International Medical Journal of Experimental and Clinical Research, 26, e923914-1-e923914-9.

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Molecular Regulation of Cardiac Function

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MiR-33a-5p mimics (mimics-miR-33a-5p), miR-33a-5p inhibitor, and their negative controls (miR-NC and inhibitor-NC, respectively) were designed and synthesized by RiboBio (Guangzhou, China). Small interfering RNAs (siRNAs) targeting USP46 and circ-NNT (si-USP46 and si-circ-NNT, respectively) and a scrambled form used as control (shRNA NC) were obtained from Dharmacon (Lafayette, CO, USA). Mimics-miR-33a-5p, mimics-NC, miR-33a-5p inhibitor, inhibitor-NC, si-USP46, circ-NNT shRNA, and shRNA NC were transfected into cultured cardiomyocytes using Lipofectamine® 2000 (Invitrogen) following manufacturer’s instructions. The circ-NNT exon along with the endogenous flanking sequence (1 kb upstream) was inserted into the pcDNA3.1 vector, and part of the upstream flanking sequence was inserted in an inverted orientation downstream. The mouse USP46 was cloned by PCR using mouse cDNA as the template and inserted into the pcDNA3.1 vector. All vectors (pcDNA-circ-NNT, pcDNA-USP46, and pcDNA3.1 empty vector) as well as si-USP46, circ-NNT shRNA, and shRNA NC were cloned into the Adeno-X Expression System (Clontech, Otsu, Japan) following manufacturer’s instructions. All adenoviral constructs were amplified in HEK293 cells. Adenoviral infection of HEK293 cells or cardiomyocytes was carried out as previously described [39 (link)].

Ye X., Hang Y., Lu Y., Li D., Shen F., Guan P., Dong J., Shi L, & Hu W. (2021). CircRNA circ-NNT mediates myocardial ischemia/reperfusion injury through activating pyroptosis by sponging miR-33a-5p and regulating USP46 expression. Cell Death Discovery, 7, 370.

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Investigating miR-373 Regulation of GAB2 in Lung Cancer

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miR-373 mimics, mimics negative control (NC), inhibitor and inhibitor NC were synthesized by Genepharma (Shanghai, China). The sequences are as follows: MiR-373 mimics, 5′-GAAGUGCUUCGAUUUUGGGGUGU-3′; mimics NC, 5′-GCUUUAUUAGAGUGCUAUUGCUU-3′; miR-373 inhibitor, 5′-ACACCCCAAAAUCGAAGCACUUC-3′; and inhibitor NC, 5′-AUUCUCAUCACCAAUCACGAAAUA-3′. A549 and H358 cells were transfected using Lipofectamine® 2000 (Invitrogen; Thermo Fisher Scientific, Inc.) in accordance with the manufacturer's instructions with a GAB2-overexpression pcDNA3.1 plasmid (1 µg; Invitrogen; Thermo Fisher Scientific, Inc.). As an NC, a pcDNA3.1 vector that was empty was utilized. After 48 h of transfection, the cells were harvested for the following experiments. The efficiency of overexpression or inhibition was verified by RT-qPCR and western blot.

Zhu X., Chen X., Zhang X., Zhao L, & Shen X. (2024). MicroRNA‑373‑3p inhibits the proliferation and invasion of non‑small‑cell lung cancer cells by targeting the GAB2/PI3K/AKT pathway. Oncology Letters, 27(5), 221.

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Adipocyte Differentiation and miR-27b Modulation

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After successful differentiation of 3T3-L1 pre-adipocytes, mature white adipocytes were treated with 10 mM phosphate-buffered saline (PBS; pH 7.4) and 1 or 10 µmol/l liraglutide, respectively (blank group, 1 µM liraglutide group, 10 µM liraglutide group). Mature white adipocytes were transfected with mimic-NC or miR-27b mimics (Ribobio, Guangzhou, China) using Lipofectamine® 2000 (Invitrogen, Carlsbad, CA, USA) for 6 h, and then treated with 1 µmol/l liraglutide for 48 h, with the groups named as the mock group, mimics-NC group, miR-27b mimics group, mimics-NC + liraglutide group and miR-27b mimics + liraglutide group.

Wang X., Chen S., Lv D., Li Z., Ren L., Zhu H., Xie X, & Liu Y. (2021). Liraglutide suppresses obesity and promotes browning of white fat via miR-27b in vivo and in vitro. The Journal of International Medical Research, 49(11), 03000605211055059.

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Modulating miR-103a-3p and FBXW7 in Cervical Cancer

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miR-103a-3p mimics (5′-AGCAGCATTGTACAGGGCTATGA-3′), mimics negative control (NC) (5′-ATAGTGATCAGATGGGCAGCCTA-3′), miR-103a-3p inhibitor (5′-TCATAGCCCTGTACAATGCTGCT-3′), inhibitor NC (5′-TATCCCACGCTAGCTGTCTTGAA-3′) were obtained from Shanghai GenePharma Co., Ltd. pcDNA3.1-FBXW7, pcDNA3.1 vector, si-FBXW7 (5′-GCTCCCTAAAGAGTTGGCACTCTAT-3′) or si-Scramble (5′-GCTATCGAATGAGGTCACCTCCTAT-3′) were synthesized by Guangzhou RiboBio Co, Ltd. (Guangzhou, China). Then, SiHa and Hela cells (4 × 10⁵ cells per well) were seeded in 6-well plates and cultured overnight until reached about 80% confluence, 100 nM miR-103a-3p mimics, mimics NC, miR-103a-3p inhibitor, inhibitor NC, 2 μg pcDNA3.1-FBXW7 plasmid, and 100 ng si-FBXW7 were transfection using the Lipofectamine 3000 reagent (Invitrogen, Thermo Fisher Scientific, Inc., Waltham, MA, USA) according to the manufacturer’s protocol. After incubation for 6 h, regular culture medium was added into each well to terminate reaction at 37 °C. 48 post-transfection, cells were harvested for subsequent experiments.

Ren L., Yang J., Meng X., Zhang J, & Zhang Y. (2022). The promotional effect of microRNA-103a-3p in cervical cancer cells by regulating the ubiquitin ligase FBXW7 function. Human Cell, 35(2), 472-485.

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Regulating EZH2 Expression in Oral Cancer Cells

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The miR-144-3p mimics and mimics negative control (mimics NC) were obtained from GenePharma. The EZH2 overexpression vector, pcDNA-EZH2, and pcDNA vector were constructed by GenePharma. In addition, EZH2 siRNA (si-EZH2) and si-Scramble were also purchased from GenePharma.
CAL-27 and SCC-4 cells (8.0×10⁵/well) in a 6-well plate grown to approximately 80% confluence, and then respectively transfected with miR-144-3p mimics (20 nM), mimics NC (20 nM), si-EZH2 (50 nM), or 2 µg pcDNA-EZH2 using Lipofectamine^® 2000 (Invitrogen; Thermo Fisher Scientific, Inc.). In addition, inhibition experiments were performed using the EZH2 inhibitor, GSK126 (a compound competes with S-adenosyl-methionine for binding to EZH2, thereby inhibiting histone methyltransferase activity without affecting EZH2 protein expression), as previously described (16 (link)). Briefly, the CAL-27 and SCC-4 cells were treated with 5 µM GSK126 (Shanghai HanXiang Life Technology Limited Corporation) or DMSO for 48 h and then collected for use in further experiments. The concentration of DMSO used was ≤1% to ensure the lack of cytotoxicity.

He L., Liao L, & Du L. (2020). miR-144-3p inhibits tumor cell growth and invasion in oral squamous cell carcinoma through the downregulation of the oncogenic gene, EZH2. International Journal of Molecular Medicine, 46(2), 828-838.

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Exploring L-OHP Sensitivity in HeLa Cells

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After preparing cell suspensions, we added 5, 10, 15, 20, 25, and 30 µM concentrations of L-OHP (Sigma-Aldrich, San Francisco, CA, USA) cell medium. Cells were treated with L-OHP for 48 hours and changed into fresh medium. Hela/L-OHP cells were cultured with the 50% maximal inhibitory concentration (IC50) of transfected cells against L-OHP for 3 hours to assess the chemical sensitivity of the drug. After treatment with drug, cells were subjected to further experiments. miR-34a-5p mimics, mimics-NC, miR-34a-5p inhibitor, and inhibitor-NC were purchased from Taitool Bioscience (Shanghai, China). si-NC, si-MDM4, ov-NC, and ov-MDM4 were designed and synthesized by Bio-Rad (Hercules, CA, USA). Then, Hela/L-OHP cells were transfected with miR-34a-5p mimics, mimics-NC, miR-34a-5p inhibitor, inhibitor-NC, si-NC, si-MDM4, ov-NC, and ov-MDM4 by using Lipofectamine 3000 reagent (Invitrogen, Carlsbad, CA, USA) as per instructions.

He P.T., Liu X., Lou Y., Gong S., & Cao L. (2022). miR-34a-5p enhances the sensitivity of cervical cancer cells to oxaliplatin chemotherapy via targeting MDM4. Clinical and Experimental Obstetrics & Gynecology, 49(2), 054-054.

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Regulation of HepG2 cells by miR-450

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HepG2 cells were bought from Chinese Academy of Sciences cell bank. The cells were cultured in DMEM medium (Thermo Fisher Scientific, Waltham, MA, USA) containing 5% FBS, and incubated at 37°C with 5% CO₂. Cultured cells were divided into miR-450 mimics group, miR-450 inhibitor group, miR-450 mimics NC group, miR-450 inhibitor NC group andblank group and transfected with miR-450 mimics, miR-450 inhibitor miR-450 mimics NC, miR-450 inhibitor NC, and no treatment was done, respectively. Cell transfection was performed according to the lipofectamine^TM 2000 (Thermo Fisher Scientific, Waltham, MA, USA) transfection instructions. The successfully transfected HepG2 cells were prepared into cell suspensions using the same medium as in culture, and then inoculated in 24-well plates with 1×10⁵ cells/well. Then, the cells were cultured in an incubator at 37°C, 5% CO₂, and 95% humidity. miR-450 mimics, miR-450 inhibitor, mimics NC, and miR-450 inhibitor NC were synthesized by ThermoFisher scientific (Waltham, USA).

Wang Y., Wang L., Yu X, & Duan J. (2019). Overexpression of miR-450 affects the biological behavior of HepG2 cells by targeting DNMT3a. OncoTargets and therapy, 12, 5069-5076.

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Investigating miR-181a Regulation in Cancer Cells

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The miR-181a mimics, mimics negative control (mimics NC), miR-181a inhibitor and inhibitor negative control (inhibitor NC) were purchased from Sangon Biotech Co., Ltd. Details of the miRNAs, their inhibitors and controls are shown in Table I. Briefly, ECA109 and TE-1 cells were first seeded into 6-well plates (5×10⁵ cells/well). After reaching 40–50% confluence, Lipofectamine 6000^® (Invitrogen; Thermo Fisher Scientific, Inc.) was used to transfect with miR-181a mimics (20 µM), mimics NC (20 µM), miR-181a inhibitor (20 µM) and inhibitor NC (20 µM) in DMEM supplemented with 10% fetal bovine serum without 1% penicillin/streptomycin for 6 h at 37°C. Negative control (NC) served as the control group. The medium was replaced after 6 h to remove the remaining liposomes. After 48 h transfection, cells were collected for RT-qPCR analysis and after 72 h for western blot analysis.

Xu R., Zhou X.M., Li Y.S., Ren L, & He X.R. (2021). MicroRNA-181a promotes epithelial-mesenchymal transition in esophageal squamous cell carcinoma via the TGF-β/Smad pathway. Molecular Medicine Reports, 23(5), 316.

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