Humancoreexome 24 v1.0 manifest file

Twenty-three healthy volunteers were recruited at the University of Manchester as part of the National Repository Healthy Volunteers (NRHV) study. All the samples were collected with ethical committee approval (MREC 99/8/84) and all individuals provided informed consent. The median age of the subjects was 50.5 years (range=26–82 years) with eight males and 15 females. A total of 20 ml peripheral blood was collected in Vacutainer plus tubes containing EDTA (Becton Dickinson). PBMCs were extracted within 2 h of collection from 20 ml of whole blood using Ficoll Plus density gradient centrifugation (GE Healthcare). Cells were washed twice with MACS running buffer (phosphate-buffered saline (PBS), bovine serum albumin, EDTA and sodium azide; Miltenyi) and total PBMCs counted using the CASY cell counter (Roche). To allow collection of all the samples, PBMC samples were immediately cryopreserved in 1 ml recovery cell-freezing medium (Gibco) per 5 × 10⁶ cells at a cooling rate of −1 °C per minute. Samples were genotyped using the Illumina HumanCoreExome v1.0 array, in accordance with the manufacturer’s instructions. Genotype clustering and calling was performed using the GenomeStudio Data Analysis software platform using the Illumina HumanCoreExome-24 v1.0 Manifest File.