EZMagna RNA immunoprecipitation (RIP) kit (Millipore, Billerica, MA, U.S.A.) was applied for conducting RIP experiments. Molt-3 or Molt-4 cells were cultured in RIP buffer containing magnetic beads conjugated with human anti-Ago2 antibody (Millipore) or anti-IgG (Millipore). Besides, normal IgG and input were seen as the negative and positive controls, respectively. Moreover, the purified RNAs were subjected to qRT-PCR analysis.
Ezmagna rna immunoprecipitation rip kit
Manufactured by Merck Group
Sourced in United States
The EZMagna RNA immunoprecipitation (RIP) Kit is a laboratory equipment product designed for the isolation and purification of RNA-binding proteins and their associated RNA complexes. The kit provides reagents and protocols for performing RNA immunoprecipitation experiments to study protein-RNA interactions.
Automatically generated - may contain errors
Lab products found in correlation
Human anti ago2 antibody, by Merck Group (1 mentions)
Ab186733, by Cell Signaling Technology (1 mentions)
Normal mouse igg, by Merck Group (1 mentions)
Human anti argonaute2 ago2 antibody, by Merck Group (1 mentions)
Ago2 antibody, by Merck Group (1 mentions)
Ab172730, by Abcam (1 mentions)
Ab186733, by Abcam (1 mentions)
13 protocols using ezmagna rna immunoprecipitation rip kit
1
Ago2-RIP protocol for RNA analysis
2
RIP Assay for Linc01503 Interactome
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The interactions between linc01503 and potential binding proteins were detected by RIP assays using the EZ‐Magna RNA Immunoprecipitation (RIP) Kit (Millipore) under the instructions of the manufacturer. The details are summarized in Appendix S1 .
3
AGO2-Mediated RNA Immunoprecipitation
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An EZ‐Magna RNA immunoprecipitation (RIP) Kit (Millipore, 17–701) was used according to the manufacturer's instructions. Briefly, 5 × 106 BMSCs were lysed and magnetic beads conjugated with anti‐AGO2 (Abcam, ab186733, 1:50) or negative control IgG (Cell Signaling Technology, 2729S, 1:50) were added. The immunoprecipitated RNAs and the input RNAs were extracted and subjected to qPCR followed by electrophoresis to detect the expression of HHAS1.
4
Ago2 RIP Profiling of ncRNAs
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The EZMagna RNA immunoprecipitation (RIP) Kit (Millipore, USA) was used according to the manufacturer’s protocol. HNE1 and C666-1 cells were lysed and incubated with RIPA buffer containing magnetic beads conjugated with a human anti-Argonaute2 (Ago2) antibody (Millipore). Normal mouse IgG (Millipore) was used as a negative control. The samples were incubated with proteinase K, after which immunoprecipitated RNA was extracted. Purified RNA was subjected to qRT-PCR analysis.
5
NLRP3 RNA Immunoprecipitation Assay
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An EZ-Magna RNA immunoprecipitation (RIP) kit (Millipore, MA) was used to perform the RIP assay. Briefly, NR8383 AM cells (1 × 107) were collected and incubated with RIP lysis buffer. The precleared lysates were used for RIP with anti-NLRP3 (Boster Biotech; BA3677, 1 : 200, China) and rabbit isotype control IgG antibodies. RNA was isolated and purified using an acid phenol/chloroform method.
6
AGO2-RIP Transcriptome Analysis
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An EZ-Magna RNA Immunoprecipitation (RIP) Kit (Millipore, United States) was taken to AGO2-RIP experiments. The HEK-293 cells were lysed and incubated with human anti-Ago2 or mouse IgG-coated beads (Millipore, United States). qRT-PCR was used to analyze the immunoprecipitated RNAs.
7
AGO2 RNA Immunoprecipitation Protocol
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An EZMagna RNA immunoprecipitation (RIP) Kit (Millipore, Billerica, USA) was used according to the manufacturer’s protocol. Antibody (anti-AGO2) for RIP assay was from Abcam.
8
RNA Immunoprecipitation Using EZMagna Kit
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The EZMagna RNA immunoprecipitation (RIP) kit (Millipore) was used according to the manufacturer's protocol. U87 cells were lysed in complete RIP lysis buffer, and the cell extract was incubated overnight at 4°C with magnetic beads conjugated with specific antibodies or control IgG. The beads were washed in PBS three times and incubated with proteinase K to remove proteins. Finally, purified RNA was used to perform the qRT-PCR analysis.
9
RNA Immunoprecipitation Assay Protocol
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An EZMagna RNA immunoprecipitation (RIP) Kit (Millipore, Bedford, MA, USA) was used following the manufacturer’s protocol. BGC-823 and SGC-7901 cells were lysed in complete RIP lysis buffer (containing proteinase inhibitor and phosphatase inhibitor), and the cell extract was incubated with magnetic beads conjugated with specific antibodies or control IgG for 6 h at 4 °C. Beads were washed and incubated with proteinase K to remove proteins. Finally, purified RNA was subjected to qRT-PCR analysis.
10
Exploring circRNA-miRNA Interactions via RIP
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With the aid of the EZMagna RNA immunoprecipitation (RIP) Kit (Millipore, USA), RIP assay was carried out following the product manuals. After transfection, THCA cells were dissolved in RIP lysis buffer. Next, the lysates were immunoprecipitated by using magnetic beads and Ago2 antibody (Millipore) or negative control IgG antibody (Millipore). Following incubating all the night at 4℃, RNA‐protein mixtures were eluted from magnetic beads, and thereafter Proteinase K treatment was used for RNA isolation. The abundance of hsa_circ_0000285 and miR‐127‐5p was analyzed with qRT‐PCR assay.
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