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Rabbit or goat serum

Manufactured by Agilent Technologies
Sourced in Denmark

Rabbit or goat serum is a laboratory product used as a standard or control for various bioassays and research applications. It is a sterile-filtered serum derived from the blood of rabbits or goats. The serum can be used to supplement cell culture media or as a component in immunoassays and other analytical procedures.

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2 protocols using rabbit or goat serum

1

Immunohistochemistry Protocol for Protein Detection

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After dewaxing in xylene and rehydration in graded alcohols, tissue sections were boiled in citrate buffer, pre-incubated with H2O2, and blocked with rabbit or goat serum (DAKO, Glostrup, Denmark). Sections were then incubated with a primary antibody and then with an HRP-conjugated secondary antibody. Proteins of interest were visualized using diaminobenzidine before counterstaining with hematoxylin. Antibodies used were as following: LPCAT1 (16112–1-AP, Proteintech), PCNA (10205–2-AP, Proteintech), CD34 (ab81289, Abcam, Cambridge, MA, USA) and MMP9 (10375–2-AP, Proteintech).
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2

Immunohistochemical Analysis of Biomarkers

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After dewaxing in xylene and rehydration in graded alcohols, the tissue sections were boiled in citrate buffer, pre-incubated with H2O2, and then blocked with rabbit or goat serum (DAKO, Denmark). Subsequently, the sections were incubated with a primary antibody and then with an HRP-conjugated secondary antibody. The proteins of interest were visualized by using diaminobenzidine before counterstaining with hematoxylin. The primary antibodies used were as following: MIF (ab7207, Abcam, USA), CD68 (ab283654, Abcam, USA) and HIF-α (ab51608, Abcam, USA).
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