Alkynyl myristic acid (YnMyr, CAS No. 82909-47-5) was obtained from Click Chemistry Tools. Azo biotin-azide (CAS No. 1339202-33-3) and Imatinib (Cas No.152459-95-5) were obtained from Sigma Aldrich, rapamycin (Cas No.53123-88-9) from ApexBio (Huston, TX), AP21967 (rapalog) from Takara, Doxycycline (Cas No.24390-14-5) from Stem Cell Technologies, and SGK1 inhibitor GS-9007 (Cas No.1426214-51-8) was obtained from Cayman Chemical. All commercially obtained chemicals were dissolved in DMSO and used without further purification.
Doxycycline
Manufactured by STEMCELL
Doxycycline is a tetracycline antibiotic used in cell culture applications. It is a broad-spectrum antibiotic that inhibits bacterial protein synthesis.
Automatically generated - may contain errors
2 protocols using doxycycline
1
Synthesis and Characterization of Alkynyl Myristic Acid Derivatives
2
Reprogramming GBM Cells into ic-GSCs
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FUW-tetO-hOKMS (Addgene plasmid #51543; RRID: Addgene_51543) was a gift from Tarjei Mikkelsen [17 (link)] and the FUW-M2rtTA (Addgene plasmid #20342; RRID: Addgene_20342) was a gift from Rudolf Jaenisch [18 (link)]. GBM-DCs were reprogrammed into ic-GSCs using the iPSC generation protocol [18 (link)]. Briefly, VSVG coated lentiviruses were packaged in 293 T cells cultured in mTeSR1 media. FUW-OKMS and FUW-M2rtTA viral supernatants were mixed at a 1:1 ratio, and 0.3 × 106 GBM-DCs were infected four times for 48h. Transduced GBM-DCs were treated with doxycycline 2 μg/mL (#72742, STEMCELL Technologies) to induce OKMS expression until ic-GSC colonies appeared after four weeks. Single-cell clone selection was conducted following manual serial dilution in 96-well plates.
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