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E14 cortical progenitor cells

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E14 cortical progenitor cells are a type of primary cell derived from the embryonic day 14 cortex of the mouse. These cells represent a population of neural progenitor cells that can differentiate into various cell types of the cerebral cortex, including neurons and glia.

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Lab products found in correlation

Poly l ornithine, by Merck Group (2 mentions) Laminin, by Merck Group (2 mentions) Progesterone, by Thermo Fisher Scientific (1 mentions) Insulin, by Thermo Fisher Scientific (1 mentions) Putrescine, by Thermo Fisher Scientific (1 mentions) Human transferrin, by Thermo Fisher Scientific (1 mentions) Dmem f12 with glutamax, by Thermo Fisher Scientific (1 mentions) Human basic fibroblast growth factor, by R&D Systems (1 mentions) Human epidermal growth factor (hegf), by R&D Systems (1 mentions) Dibutyryl camp, by Merck Group (1 mentions) Neurobasal medium, by STEMCELL (1 mentions)

2 protocols using e14 cortical progenitor cells

1

Maintaining Cortical Progenitor Cells

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E14 cortical progenitor cells (R&D systems) were seeded onto 15μg/ml Poly-L-ornithine (Sigma) and 1μg/ml laminin (Sigma) coated 6 well plates as a monolayer culture. Cell culture medium was composed of DMEM/F-12 with glutamax (Life Technologies), 1X N2 supplement composed of Insulin, Human Transferrin, Putrescine, Selenite and Progesterone (Life Technologies) and glucose (Sigma). Culture medium was supplemented with 10ng/ml of human basic fibroblast growth factor (R&D systems) and 10ng/ml of human epidermal growth factor (R&D systems) every day until cell lysate collection to maintain cortical progenitors cells in an undifferentiated state.
Mishra S., Choe Y., Pleasure S.J, & Siegenthaler J.A. (2016). Cerebrovascular defects in Foxc1 mutants correlate with aberrant WNT and VEGF-A pathways downstream of retinoic acid from the meninges. Developmental biology, 420(1), 148-165.
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2

Cortical Progenitor Cell Differentiation

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E14 cortical progenitor cells (R&D systems) were seeded onto 20μg/ml Poly-L-ornithine (Sigma) and 10μg/ml laminin (Sigma) coated 6 well plates as a monolayer culture. After 2 days, progenitor medium (as described above) was changed to neural differentiation medium which was composed of Neurobasal medium (Stem Cell Technologies), B27 serum free supplement (2%,Life technologies) and GlutaMAX™-I Supplement (2mM Life technologies). Differentiation medium was changed every 3-4 days and 0.5 mM of dibutyryl cAMP (Sigma) was added starting on day 7 of differentiation for 3 days.
Mishra S., Choe Y., Pleasure S.J, & Siegenthaler J.A. (2016). Cerebrovascular defects in Foxc1 mutants correlate with aberrant WNT and VEGF-A pathways downstream of retinoic acid from the meninges. Developmental biology, 420(1), 148-165.
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