Chemicals: PP2 inhibitor (Tocris), propidium iodide (PI) and Hoechst stains (Biolegend), LT-1 transfection reagent (Mirus). For imaging analysis, #0030730119 (tissue culture (TC) treated) and #0030730011 (non-TC culture treated) Eppendorf 96-well plates were used. Non-TC treated plates were used for agonistic antibody adsorption. Sterile carrier-free anti-CD3 (2C11) and anti-CD28 (37.51) were obtained from Biolegend. Fluorescent anti-TCRβ (AF647) (H57–597) and anti-CD8α (PE) (53–6.7) were obtained from Biolegend. Streptavidin nanospheres were obtained from Biolegend (#480016). Biotinylated antibodies used for negative selection cocktail were obtained from Biolegend: Biotin anti-CD11b (M1/70) (#101204), Biotin anti-CD11c (N418) (#117304), Biotin anti-CD19 (6D5) (#115504), Biotin anti-CD24 (M1/69) (#101804), Biotin anti-CD45R (B220) (RA3-6B2) (#103204), Biotin anti-CD49b (DX5) (#108904), Biotin anti-TER119 (clone TER-119) (#116204), Biotin CD4 (GK1.5) (#100404). Antibodies (0.5 mg each, except for 0.1 mg anti-CD11c) were pooled and dialyzed in PBS before sterile filtration and storage at 80 °C.
Lt 1 transfection reagent
Manufactured by BioLegend
LT-1 transfection reagent is a cationic lipid-based formulation designed for efficient DNA and RNA delivery into a wide range of cell types. It facilitates the uptake of nucleic acids by forming complexes that can penetrate the cell membrane.
Automatically generated - may contain errors
Lab products found in correlation
Anti cd28 37, by BioLegend (2 mentions)
Propidium iodide, by BioLegend (2 mentions)
Pp2 inhibitor, by BioLegend (2 mentions)
Sterile carrier free anti cd3 2c11, by BioLegend (2 mentions)
Fluorescent anti tcrβ af647 h57 597, by BioLegend (2 mentions)
Anti cd8α pe 53 6.7, by BioLegend (2 mentions)
Streptavidin nanospheres, by BioLegend (2 mentions)
Biotin anti cd11b m1 70, by BioLegend (2 mentions)
Biotin anti cd11c n418, by BioLegend (2 mentions)
Biotin anti cd19 6d5, by BioLegend (2 mentions)
Biotin anti cd24 m1 69, by BioLegend (2 mentions)
Biotin anti cd45r b220 ra3 6b2, by BioLegend (2 mentions)
Biotin anti cd49b dx5, by BioLegend (2 mentions)
Biotin anti ter119 clone ter 119, by BioLegend (2 mentions)
Biotin cd4 gk1.5, by BioLegend (2 mentions)
2 protocols using lt 1 transfection reagent
1
Isolation and Activation of Murine T Cells
2
Isolation and Activation of Murine T Cells
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Chemicals: PP2 inhibitor (Tocris), propidium iodide (PI) and Hoechst stains (Biolegend), LT-1 transfection reagent (Mirus). For imaging analysis, #0030730119 (tissue culture (TC) treated) and #0030730011 (non-TC culture treated) Eppendorf 96-well plates were used. Non-TC treated plates were used for agonistic antibody adsorption. Sterile carrier-free anti-CD3 (2C11) and anti-CD28 (37.51) were obtained from Biolegend. Fluorescent anti-TCRβ (AF647) (H57–597) and anti-CD8α (PE) (53–6.7) were obtained from Biolegend. Streptavidin nanospheres were obtained from Biolegend (#480016). Biotinylated antibodies used for negative selection cocktail were obtained from Biolegend: Biotin anti-CD11b (M1/70) (#101204), Biotin anti-CD11c (N418) (#117304), Biotin anti-CD19 (6D5) (#115504), Biotin anti-CD24 (M1/69) (#101804), Biotin anti-CD45R (B220) (RA3-6B2) (#103204), Biotin anti-CD49b (DX5) (#108904), Biotin anti-TER119 (clone TER-119) (#116204), Biotin CD4 (GK1.5) (#100404). Antibodies (0.5 mg each, except for 0.1 mg anti-CD11c) were pooled and dialyzed in PBS before sterile filtration and storage at 80 °C.
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