Ferrostatin 1 fer 1 s7243

Anti-MFG-E8 (sc-271574; 1:200 dilution for immunoblotting, immunofluorescence, and immunohistochemistry; 2 µg per 1 mg of total protein for immunoprecipitation), anti-USP14 (sc-398009, 1:200 for immunoblotting, 2 µg per 1 mg of total protein for immunoprecipitation), and anti-GPx4 (sc-166570, 1:400 for immunoblotting) antibodies were purchased from Santa Cruz Biotechnology, USA. Anti-SLC7A11 (12691, 1:1000 for immunoblotting) and anti-Ub (3936, 1:1000) were from Cell Signaling Technology, USA. Anti-Flag (F7425, 1:1000) was from Sigma-Aldrich, USA. Antibodies against β-actin (60008–1-Ig, 1:5000), β-tubulin (10068-1-AP, 1:1000), and glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (60004-1-Ig, 1:1000) were from Proteintech, China. Anti-USP14 (AF301319, 1:100 for immunofluorescence) was from AiFang Biological, China. Anti-GPx4 (ab125066, Abcam, UK, 1:100), and anti-SLC7A11 (26864-1-AP, Proteintech, China, 1:50) antibodies were used for immunohistochemistry (IHC) staining. Immobilized protein A/G beads (45350) were purchased from Thermo Fisher Scientific, USA. Control IgG (sc-2025) came from Santa Cruz Biotechnology, USA. Cycloheximide (CHX) (S7418), RSL3 (S8155), and Ferrostatin-1 (Fer-1) (S7243) were from Selleck, China. MG132 (HY-13259) was purchased from MedChemExpress, USA.

DL-homocysteine (Hcy, H4628) was purchased from Sigma–Aldrich (St. Louis, MO, USA). Rituximab (RTX, 10 mg/ml), a monoclonal anti-human CD20 antibody, was purchased from Roche (Basel, Switzerland). Ferrostatin-1 (Fer-1, S7243) and liproxstatin-1 (lip-1, S7699) were purchased from Selleckchem (Houston, TX, USA). The following antibodies were used in this work. Anti-β₂GPI (bs-1570R) was purchased from Bioss Inc. (Beijing, China). Anti-CBS (cystathionine β-synthase, 14787-1-AP) and anti-CSE (cystathionine γ-lyase, 12217-1-AP) were purchased from Proteintech (Rosemont, IL, USA). Anti-CD31 (sc-18916), anti-nephrin (sc-377246) and anti-PDGFRβ (platelet-derived growth factor receptor beta, sc-374573) were purchased from Santa Cruz Biotech (CA, USA). Anti-4-HNE (4-hydroxynonenal, MAB3249) was purchased from R&D Systems (MN, USA). Anti-xCT (catalytic subunit of cystine/glutamate antiporter System Xc-, also called SLC7A11, ab37185), anti-LOX15 (Lipoxygenase 15, ab23691), anti-GPX4 (glutathione peroxidase 4, ab125066), and anti-TFR (transferrin receptor, ab214039) were purchased from Abcam (Cambridge, MA, USA). Anti-TF (transferrin, A1448), anti-SLC40A1 (A14884), anti-β-actin (AC038), HRP-conjugated goat anti-rabbit (AS014), and HRP-conjugated goat anti-mouse (AS003) were purchased from ABclonal (Wuhan, China).

Ferroptosis agonists Erastin (S7242, 4.6 μM) and RSL3 (SIM; S8155, 0.5 µM), and ferroptosis inhibitors ferrostatin-1 (Fer-1; S7243, 5 μM) and liproxstatin-1 (Lip-1; S7699, 22 μM) were purchased from Selleck (Shanghai, China).