Rabbit igg i5006

Manufactured by Merck Group

Sourced in United States

Rabbit IgG(I5006) is a laboratory reagent produced by Merck Group. It is a purified immunoglobulin G (IgG) fraction isolated from rabbit serum. The core function of this product is to serve as a reference standard or control material for immunoassays and other applications requiring rabbit IgG.

Automatically generated - may contain errors

Lab products found in correlation

Myc sc 40, by Santa Cruz Biotechnology (1 mentions) Ab23980, by Abcam (1 mentions) Anti h3k4me3 07 473, by Merck Group (1 mentions) Anti h3k27ac 39133, by Active Motif (1 mentions) Anti cdk2 sc 163, by Santa Cruz Biotechnology (1 mentions) Anti ha ha 11 clone 16b12, by Fortrea (1 mentions) Fl 393, by Santa Cruz Biotechnology (1 mentions)

Spelling variants of this product

I5006 (23 citations) IgG rabbit (4 citations) Rabbit IgG (Cat No. I5006) (2 citations)

4 protocols using rabbit igg i5006

Antibody and Compound Source Protocols

Check if the same lab product or an alternative is used in the 5 most similar protocols

Antibodies and their commercial sources are as follows: PRMT5 (ab109451) and gp130 (ab202850) from Abcam; p‐STAT3 (9145), STAT3 (9139), HA (3724) and sdme‐RG (13222) from Cell Signaling Technology; SYM10 (07‐412) from Merck millipore; FLAG (F3165), β‐actin (A5441), mouse IgG (I5381), and rabbit IgG(I5006) from Sigma‐Aldrich (USA); MYC (sc‐40) from Santa Cruz Biotechnology; Survivin (A19663) and c‐MYC (A1309) from Abclonal.
The following chemical compounds and recombinant proteins were commercially obtained: EPZ015666 (S7748) and GSK591 (S8111) from Selleck; Human IL‐6 (96‐200‐06‐20) from Peprotech and TGF‐β (TGFB1‐100) from StemRD.

Cai C., Gu S., Yu Y., Zhu Y., Zhang H., Yuan B., Shen L., Yang B, & Feng X. (2021). PRMT5 Enables Robust STAT3 Activation via Arginine Symmetric Dimethylation of SMAD7. Advanced Science, 8(10), 2003047.

+ Open protocol

+ Expand

Antibody Validation for Protein Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

The following antibodies were used: anti-SKI (sc-9140, Santa Cruz); anti-RUNX1 (ab23980, Abcam); anti-CDK2 (sc-163, Santa Cruz); anti-Flag (F3163, Sigma); anti-HA (HA.11 clone 16B12; Covance); anti-H3K4me3 (07-473, Millipore); anti-H3K27ac (39133, Active Motif); rabbit IgG (I5006, Sigma).

Feld C., Sahu P., Frech M., Finkernagel F., Nist A., Stiewe T., Bauer U.M, & Neubauer A. (2018). Combined cistrome and transcriptome analysis of SKI in AML cells identifies SKI as a co-repressor for RUNX1. Nucleic Acids Research, 46(7), 3412-3428.

+ Open protocol

+ Expand

Whole-Brain Perfusion Fixation

Check if the same lab product or an alternative is used in the 5 most similar protocols

Pups were subjected to transcardiac perfusion with 0.5 mg/ml rabbit IgG (I-5006, Sigma) in Dulbecco’s PBS (-) (16220015, KOHJIN BIO). After 5 min, brains were removed and immersion fixed in 4% PFA in 0.1 M sodium phosphate buffer (pH 7.4) at 4 ℃ with gentle agitation for 2 h.

Morimoto K., Takahashi R., Takahashi G., Miyajima M, & Nakajima K. (2024). Maternal immunoglobulins are distributed in the offspring’s brain to support the maintenance of cortical interneurons in the postnatal period. Inflammation and Regeneration, 44, 24.

+ Open protocol

+ Expand

Immunoprecipitation and Chromatin Profiling of p53

Check if the same lab product or an alternative is used in the 5 most similar protocols

p53 was detected using a combination of mAb 1801/mAb DO-1 (both in-house-purified from hybridoma supernatants) or with polyclonal FL393 (Santa Cruz Biotechnology, sc-6243). Anti-p53 PAb421 (in-house-purified) was used along with mAb 1801/mAb DO-1 to deplete p53 in the ID-ChIP. Anti-Actin (A2066), mouse IgG (I5381), and rabbit IgG (I5006) antibodies were purchased from Sigma. Anti-VEGFR2 (55B11) rabbit mAb was purchased from Cell Signaling Technology. Anti-BAF53A (ab131272), anti-Histone H3 (ab1791), and anti-Histone H2A antibodies (ab18255) were purchased from Abcam. Anti-BRG1 (G-7; sc-17796), BRM (N-19; sc-6450), BAF170 (H-116; sc-10757), and BAF155 (H-76; sc-10756) antibodies were purchased from Santa Cruz Biotechnology.

Pfister N.T., Fomin V., Regunath K., Zhou J.Y., Zhou W., Silwal-Pandit L., Freed-Pastor W.A., Laptenko O., Neo S.P., Bargonetti J., Hoque M., Tian B., Gunaratne J., Engebraaten O., Manley J.L., Børresen-Dale A.L., Neilsen P.M, & Prives C. (2015). Mutant p53 cooperates with the SWI/SNF chromatin remodeling complex to regulate VEGFR2 in breast cancer cells. Genes & Development, 29(12), 1298-1315.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!