Hydrochloric acid hcl

All reagents were of analytical grade, and water was obtained with a Milli-Q system from Millipore (Bedford, MA, USA). Acetonitrile (ACN) was from Scharlau (Barcelona, Spain), di-sodium tetraborate decahydrate (borate buffer) from Merck (Darmstadt, Germany), and standard amino acids (AA-18) from Fluka (Buchs, Switzerland). Glutaraldehyde, sodium borohydrate (NaHB4), sodium tetraborate, β-mercaptoethanol, o-phthaldialdehyde (OPA), L-glutathione (GSH), and bovine serum albumin (BSA) were acquired from Sigma-Aldrich (St Louis, MO, USA). Hydrochloric acid (HCl) and calcium chloride (CaCl2) were from Panreac (Darmstadt, Germany). Alcalase was kindly donated by Novozymes Spain S.A. (Madrid, Spain).

Chemicals including 1-chloro-2,4-dinitrobenzene (CDNB), 2,3-bis(2-methoxy-4-nitro-5-sulphophenyl)-2H-tetrazolium-5-carboxanilide (XTT), 2,6-dichloroindophenol (2,6-DCIP, sodium salt hydrate), 3-(4,5-Dimethylthiazol-2-yl)-2,5-Diphenyltetrazolium Bromide (MTT), 5-Fluorouracil (5-FU), acetonitrile (can), Bradford Reagent, cumene hydroperoxide, dimethyl sulfoxide (DMSO), flavin adenine dinucleotide disodium (FAD, salt hydrated), glutathione (GSH, reduced form), hydrogen peroxide (H₂O₂) solution, β-nicotinamide adenine dinucleotide (NAD, reduced disodium salt hydrated), β -nicotinamide adenine dinucleotide phosphate (NADPH), phosphate buffered saline (PBS), sodium chloride, sodium phosphate (NaH₂PO₄), sulforaphane (SFN), 0.1% aqueous trifluoroacetic acid (TFA) solution, and Trizma^® base were purchased from Sigma-Aldrich (Madrid, Spain). Absolute ethanol, di-sodium hydrogen phosphate anhydrous (Na₂HPO₄), and hydrochloric acid (HCl) were obtained from Panreac AppliChem (Barcelona, Spain). The Cell Counting Kit-8 (CCK-8) was purchased from Dojindo Laboratories (Munich, Germany).

Boc-PLL polypeptide was synthesized by ring opening polymerization of the ε-tert-butyloxycarbonyl-L-lysine-N-carboxy anhydride (NCA), with the following molecular weight characteristics: Mw= 11,980 Daltons, Mn = 11,365 Daltons (degree of polymerization 50) and molecular polydispersity, Mw/Mn, = 1.054. Details are reported elsewhere [32 (link)]. PAA (Mw = 450,000 Daltons) was purchased from Polysciences (Warrington, USA). N, N′-Dicyclohexylcarbodiimide (DCC, Alfa Aesar, MA, USA), 1-Hydroxybenzotriazole hydrate (HOBt, Fluka, NC, USA) were used as received. Dimethylformamide (DMF, Aldrich, MA, USA), hydrochloric acid (HCl, Panreac, Chicago, IL, USA), sodium hydroxide (NaOH, Panreac, Chicago, IL, USA), diethyl ether (DEE), tetrahydrofuran (THF), methanol and trifluoroacetic acid (TFA) were purchased from Sigma-Aldrich (Athens, Greece) and used without further purification. Deuterated solvents deuterium oxide (D₂O and deuterated dimethyl sulfoxide (DMSO-d₆)) (from Sigma-Aldrich, St. Louis, MO, USA) were used as received. Ultra-pure 3-distilled water (3D-H₂O) was obtained by means of an ELGA Medica R7/15 device.

Acetonitrile (MeCN) was obtained from Merck (Darmstadt, Germany), methanol (HPLC grade quality) from Sigma-Aldrich Chemie GmbH (Steinheim, Germany), and ultrapure water from a Milli-Q A-10 system (Millipore Iberica, Madrid, Spain).
Analytical grade ammonium hydroxide (NH₄OH, 25%) and sodium hydroxide (NaOH > 99%) were obtained from Merck (Barcelona, Spain) and hydrochloric acid (HCL, 37%) from Panreac (Barcelona, Spain).
The 13desmSPXC; 13,19-didesmSPXC and 20-methSPXG solutions in methanol were purchased from CIFGA laboratorio S.A. (Lugo, Spain)

Acetonitrile (LC-MS grade) and methanol (HPLC grade quality) were purchased from Scharlab (Spain) and VWR (Spain), respectively. Ultrapure water was obtained from a Milli-Q gradient system fed with an Elix Advantage-10 (Millipore Ibérica, Spain). Ammonium hydroxide (NH₄OH, 25%) and sodium hydroxide (NaOH > 99%) were obtained from Merck (Barcelona, Spain), and hydrochloric acid (HCl, 37%) from Panreac (Barcelona, Spain).

Acetonitrile (LC-MS grade) and methanol (HPLC grade quality) were purchased from Scharlab (Sentmenat, Spain) and VWR (Llinars del Vallés, Spain), respectively. Ultrapure water was obtained from a Milli-Q gradient system fed with an Elix Advantage-10 (Millipore Ibérica, Spain). Ammonium hydroxide (NH₄OH, 25%) and sodium hydroxide (NaOH > 99%) were obtained from Merck (Barcelona, Spain), and hydrochloric acid (HCl, 37%) from Panreac (Barcelona, Spain).
The OA certified solution was acquired from the Institute for Marine Biosciences, National Research Council (NRC), Halifax, Nova Scotia, Canada.

The monomers N-isopropylacrylamide (NIPAM) and N-tert-butylacrylamide (NtBAM) were used as acquired by Fluorochem (Derbyshire, UK) and Alfa Aesar (Ward Hill, MA, USA), respectively. Potassium peroxodisulfate (KPS, Fluorochem, Derbyshire, UK), 2-aminoethanethiol hydrochloride (ET HCl, Alfa Aesar, Ward Hill, MA, USA), N-(3-Dimethylaminopropyl)-N′-ethylcarbodiimide hydrochloride (EDC, Alfa Aesar, Ward Hill, MA, USA) and 1-Hydroxybenzotriazole hydrate (HOBt, Fluka, NC, USA) were used as received. Dimethylformamide (DMF, Aldrich, St. Louis, MO, USA), hydrochloric acid (HCl, Panreac, IL, USA), sodium hydroxide (NaOH, Panreac, IL, USA), deuterated water (D₂O, Sigma-Aldrich, St. Louis, MO, USA) and Dulbecco’s modified Eagle’s medium (DMEM, Sigma-Aldrich, St. Louis, MO, USA) were used as obtained by the provider without purification. Ultrapure water was received by an ELGA Medica-R7/15 device (ELGA Labwater, IL, USA).
Sodium alginate (NaALG, Sigma-Aldrich, St. Louis, MO, USA, no. 180947, molecular weight range: 120,000–190,000 g/mol, the ratio of mannuronic and guluronic units (M/G): 1.53) was further purified (a solution of 7% w/v NaALG in NaOH (0.005 M) was repeatedly purified by dialysis against ultrapure water (membrane MWCO: 12,000–14,000 Da), and the final NaALG product was obtained in its solid-state through lyophilization.