Cell observer widefield microscope

Manufactured by Zeiss

The Zeiss Cell Observer widefield microscope is a versatile laboratory instrument designed for imaging and analysis of cellular samples. It provides high-quality, wide-field imaging capabilities for research applications. The core function of the Cell Observer is to capture detailed, high-resolution images of cell cultures and other biological specimens.

Automatically generated - may contain errors

Lab products found in correlation

Lsm 710, by Zeiss (2 mentions) H 7650 microscope, by Hitachi (1 mentions) Mitotracker green, by Thermo Fisher Scientific (1 mentions) Axiocam, by Zeiss (1 mentions) Phenol red free dmem, by Thermo Fisher Scientific (1 mentions) Zen software, by Zeiss (1 mentions) Alexa fluor 647, by Thermo Fisher Scientific (1 mentions) Vectashield mounting medium, by Vector Laboratories (1 mentions) Sir dna, by Spirochrome (1 mentions)

Spelling variants of this product

Cell Observer (142 citations) Cell Observer microscope (56 citations) Widefield microscope (18 citations) Cell Observer widefield fluorescence microscope (2 citations) Cell Observer widefield fluorescent microscope (2 citations)

6 protocols using cell observer widefield microscope

Ultrastructural and Fluorescence Analysis of Trypanosomes

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Formalin-fixed organs were immunostained with a non-purified rabbit serum anti-T. brucei VSG13 antigen (which crossreacts with many VSGs) and a non-purified rabbit serum anti-T. brucei H2A. For TEM, ultra-thin sections (70 nm) were screened in a Hitachi H-7650 microscope. 3D reconstruction of isolated trypanosome was done using the IMOD software package version 4.7.3 for alignment and modeling (Kremer et al., 1996 (link)).
For fluorescence analysis, the gonadal depot was stained with LipidTox, fixed in 10% neutral-buffered formalin and embedded in Fluoromount-G. Fluorescence images were taken using a 40× objective in a Zeiss Cell Observer wide-field microscope. For morphometry analysis, isolated parasites were fixed with paraformaldehyde, DAPI stained, and embedded in vectashield. Images were taken using a 63× oil objective with optional optovar magnification (1.6×) in the same wide-field microscope. Parasite measurements were taken essentially as described in Wheeler et al. (2012) (link).
The mitochondrion of isolated parasites was labeled using MitoTracker Green (Invitrogen/Molecular Probes, M-7514) according to the manufacturer’s instructions. Fluorescence and DIC images were acquired using a confocal laser point-scanning microscope (Zeiss LSM 710).

Trindade S., Rijo-Ferreira F., Carvalho T., Pinto-Neves D., Guegan F., Aresta-Branco F., Bento F., Young S.A., Pinto A., Van Den Abbeele J., Ribeiro R.M., Dias S., Smith T.K, & Figueiredo L.M. (2016). Trypanosoma brucei Parasites Occupy and Functionally Adapt to the Adipose Tissue in Mice. Cell Host & Microbe, 19(6), 837-848.

+ Open protocol

+ Expand

Live-cell imaging of DNA replication

Check if the same lab product or an alternative is used in the 5 most similar protocols

Cells were seeded in glass bottom 12-well plates (MakTek Corporation) the day before imaging at 30% confluency, to ensure that cells were actively cycling for the duration of the experiment. Media was replaced with phenol red-free DMEM (Gibco) supplemented with 10% FCS and drug treatment immediately before transferring the plate to the microscope chamber. Imaging was performed on the Cell Observer Widefield Microscope (Zeiss) using a 20× objective at 37 °C with 10% CO₂ in a XLmulti S1 full enclosure chamber. Cells were incubated in the XLmulti S1 full enclosure chamber for 2 h prior to imaging. Three to four positions per well were captured with AxioCam (Zeiss) 506 Mono using ZEN software (Carl Zeiss) with images taken every 6 min for 60 h. The number of nuclei, interphase duration, S/G2 duration and mitotic duration (defined by duration between initial rounding up of cell to completion of cytokinesis) were recorded. More than 15 cells were analysed at each position for each condition. Live cell assays for GFP-RPA (Origene Technologies) and TMRdirect-Halotag-ZNF827 (Promega), GFP-ZNF827, and chromobody-RFP-PCNA (ChromoTek) were performed on the Cell Observer SD 48 h post-transfection using a 40× objective at 37 °C with 10% CO_2, with images taken every 5 min.

Yang S.F., Nelson C.B., Wells J.K., Fernando M., Lu R., Allen J.A., Malloy L., Lamm N., Murphy V.J., Mackay J.P., Deans A.J., Cesare A.J., Sobinoff A.P, & Pickett H.A. (2024). ZNF827 is a single-stranded DNA binding protein that regulates the ATR-CHK1 DNA damage response pathway. Nature Communications, 15, 2210.

+ Open protocol

+ Expand

Immunofluorescence Imaging of BSF Parasites

Check if the same lab product or an alternative is used in the 5 most similar protocols

BSF parasites (5 × 10⁵) were harvested by centrifugation (1800g for 10 min), washed with 1× PBS, and resuspended in 500 μl of fixation buffer (4% paraformaldehyde diluted in 1× PBS) for 10 min at room temperature. Fixed cells were washed with 500 μl of 1× PBS and resuspended with 100 μl of 1× PBS. Cells were then settled on precoated polylysine culture dishes (35-mm glass bottom, MatTek) for at least 20 min. PBS was removed, and cells were permeabilized with 100 μl of 0.1% Triton X-100 in PBS for 2 min at room temperature. Permeabilized cells were washed five times with 200 μl of PBS and blocked with 2% bovine serum albumin (BSA) in PBS for 45 min at 37°C in a humidity chamber. Cells were incubated with 100 μl of the primary antibody anti-PAD1 (1:1000 in 2% BSA in PBS; antibody provided by K. Matthews) overnight at 4°C in a humidity chamber. Cells were washed five times with 200 μl of PBS and incubated with 100 μl of the secondary antibody anti-rabbit (1:1000 in 2% BSA in PBS; goat anti-rabbit Alexa Fluor 647, Invitrogen, #A21245) for 45 min at 4°C in a humidity chamber. Parasite DNA was stained using 100 μl of DAPI or Hoechst solution (1 μg/ml) for 20 min at room temperature. Cells were washed five times with 200 μl of PBS, and 100 μl of VECTASHIELD was added to the dishes before analysis with the Zeiss cell observer widefield microscope.

Guegan F., Rajan K.S., Bento F., Pinto-Neves D., Sequeira M., Gumińska N., Mroczek S., Dziembowski A., Cohen-Chalamish S., Doniger T., Galili B., Estévez A.M., Notredame C., Michaeli S, & Figueiredo L.M. (2022). A long noncoding RNA promotes parasite differentiation in African trypanosomes. Science Advances, 8(24), eabn2706.

+ Open protocol

+ Expand

Multimodal Microscopy Imaging Analysis

Check if the same lab product or an alternative is used in the 5 most similar protocols

Brightfield and fluorescence images were acquired at the Advanced Light Microscopy Facility at EMBL, Heidelberg with a ZEISS Cell Observer Widefield microscope, equipped with a metal halide fluorescence lamp. Observations were made using a 63× oil objective, and the acquired images were analysed with ImageJ (Schindelin et al, 2012 (link)).

Konstantinidis D., Pereira F., Geissen E., Grkovska K., Kafkia E., Jouhten P., Kim Y., Devendran S., Zimmermann M, & Patil K.R. (2021). Adaptive laboratory evolution of microbial co‐cultures for improved metabolite secretion. Molecular Systems Biology, 17(8), e10189.

+ Open protocol

+ Expand

Visualizing FLAG-tagged ANP32 Constructs

Check if the same lab product or an alternative is used in the 5 most similar protocols

Approximately 100 000 eHAP cells were cultured on sterilised glass coverslips and transfected as per minigenome reporter assay protocol. Twenty-four hours after transfection, cells were fixed in 4 % paraformaldehyde and permeabilized in 0.2 % Triton X-100. FLAG-tagged ANP32 constructs were visualised with primary antibody mouse α-FLAG (F1804; 1/200; Sigma) for 2 h at 37 °C in a humidified chamber. Cells were incubated with secondary antibody goat α-mouse Alexa Fluor-568 (1/200; Life Technologies) for 1 h at 37 °C in a humidified chamber, and counterstained with DAPI. Coverslips were mounted on glass slides using Vectashield mounting medium (H-1000–10; Vector Laboratories). Cells were imaged with a Zeiss Cell Observer widefield microscope with ZEN Blue software, using a Plan-Apochromat ×100 1.40-numerical aperture oil objective (Zeiss), an Orca-Flash 4.0 complementary metal-oxide semiconductor (CMOS) camera (frame, 2048×2048 pixels; Hamamatsu), giving a pixel size of 65 nm, and a Colibri seven light source (Zeiss). Channels acquired and filters for excitation and emission were 4′,6-diamidino-2-phenylindole (DAPI) (excitation [ex], 365/12 nm, emission [em] 447/60 nm), and TexasRed (ex 562/40 nm, em 624/40 nm). All images were analysed and prepared with Fiji software.

Staller E., Sheppard C.M., Baillon L., Frise R., Peacock T.P., Sancho-Shimizu V, & Barclay W.S. (2021). A natural variant in ANP32B impairs influenza virus replication in human cells. The Journal of General Virology, 102(9), 001664.

+ Open protocol

+ Expand

Tracking Cell Migration Dynamics

Check if the same lab product or an alternative is used in the 5 most similar protocols

MM231 SKOR1iKD cells were induced for 5 days with or without Dox, and MM231 FER ASKI cells were treated for 3 days with NM-PP1 or DMSO (control), before plating them on 24-well plasticbottom plates (Corning, Tewksbury, USA). Cells were incubated with 200 nM SiR-DNA (Spirochrome) for 7-8 hours before imaging and were imaged every 10 min for 16 h using a Carl Zeiss Cell Observer widefield microscope with an EC Plan-Neofluar 5x/0.16 M27 objective. During imaging, cells were kept in complete DMEM medium (with or without DOX, or with DMSO or NM-PP1) under normal growth conditions (37°C, 5% CO 2 ). Cell migration was quantified using the Imaris for Tracking software (Bitplane, Oxford Instruments, UK).

Sluimer L., Bullock E., Rätze M.A.K., Enserink L., Overbeeke C., Hornsveld M., Brunton V.G., Derksen P.W., & Tavares S. (2022). SKOR1 mediates FER kinase-dependent invasive growth of breast cancer cells.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!