Anti ifnγ

Manufactured by Miltenyi Biotec

Sourced in Germany

Anti-IFNγ is a laboratory product used to detect and measure the presence of interferon-gamma (IFNγ) in various samples. It serves as a tool for researchers to study immune responses and cellular functions involving IFNγ.

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Lab products found in correlation

Anti il 4, by Miltenyi Biotec (2 mentions) Phorbol myristate acetate (pma), by Merck Group (2 mentions) Ionomycin, by Merck Group (2 mentions) Anti il 4, by R&D Systems (1 mentions) Anti ifn γ, by R&D Systems (1 mentions) Human tgf β, by Thermo Fisher Scientific (1 mentions) Anti il 4, by BioXCell (1 mentions) Golgiplug protein transport inhibitor, by BD (1 mentions) Na ve cd4 t cell isolation kit, by Miltenyi Biotec (1 mentions) Anti ifn γ, by Thermo Fisher Scientific (1 mentions) Anti il 4, by Thermo Fisher Scientific (1 mentions) Human tgf β, by Miltenyi Biotec (1 mentions) Murine il 1β, by Miltenyi Biotec (1 mentions) Murine il 23, by R&D Systems (1 mentions) Murine il 2, by Thermo Fisher Scientific (1 mentions) Tgf β, by Thermo Fisher Scientific (1 mentions) Anti human cd28, by Miltenyi Biotec (1 mentions) Anti human cd3, by BioLegend (1 mentions) Anti human cd28, by BioLegend (1 mentions) Cytofix cytoperm solution, by BD (1 mentions)

Close spelling variants of this product

Anti-IFNγ-APC Anti-IFNγ PE detection reagent

4 protocols using anti ifnγ

Differentiation of Naïve CD4+ T Cells

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WT and Stat3^WT/GOF naïve CD4⁺ T cells were purified using the “Naïve CD4+ T Cell Isolation Kit” (Miltenyi Biotec, Cat. 130-104-453), cultured in supplemented RPMI (Th0, Th1, and Treg) or supplemented IMDM (Th17) media, and stimulated with plate-bound anti-CD3 (2.5ug/mL; BioXCell, 145-2C11) and soluble anti-CD28 (1ug/mL; SouthernBiotech, PV-1). T cell differentiation conditions: Th1, anti-IL-4 (10ug/mL; BioXCell, 11B11), murine IL-2 (50ng/mL; PeproTech, Cat. 212–12), and murine IL-12 (10ng/mL; R&D Sysemts, Cat. 419-ML-010); Treg, anti-IL-4, anti-IFNγ (10ug/mL; BioXCell, R4-6A2), murine IL-2, and human TGF-β (2.5ng/mL; PeproTech, Cat. 100–21); classical Th17, anti-IL-4, anti-IFNγ, human TGF-β, and murine IL-6 (30ng/mL; PeproTech, Cat. 216–16); pathogenic Th17, anti-IL-4, anti-IFNγ, human TGF-β, murine IL-1β (20ng/mL; Miltenyi Biotec, Cat. 130-094-053), and murine IL-23 (20ng/mL; R&D Systems, Cat. 1887-ML-010); Th0, anti-IL-4, anti-IFNγ, and IL-2. On day 5, cells were stimulated with phorbol-12-myristate-13-acetate (PMA; 50ng/mL; Sigma-Aldrich, Cat. 5.00582), ionomycin (1ug/mL; MilliporeSigma, Cat. 407950), and GolgiPlug Protein Transport Inhibitor (1:1000; BD Biosciences, Cat. 555029) and then stained for flow cytometry.

Woods J., Pemberton S.E., Largent A.D., Chiang K., Liggitt D., Oukka M., Rawlings D.J, & Jackson S.W. (2022). Systemic autoimmunity in murine STAT3 gain-of-function syndrome is characterized by effector T cell expansion, in the absence of overt regulatory T cell dysfunction. Journal of immunology (Baltimore, Md. : 1950).

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Differentiation of T Helper Cell Subsets

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For differentiation into T helper cell 1/2 subsets, CD4⁺ T cells were incubated for 96 h in cell culture plates covered with anti‐human CD3 (#300437, BioLegend, San Diego, CA) using TexMACS^TM medium. Cells were stimulated with T Cell TransAct^TM human as described above. Specific cytokines were added for Th1 differentiation (anti‐human CD28 [0.5 µg/ml; #302933, BioLegend, San Diego, CA], IL2 [100 U/ml], IL12 [50 ng/µl], and anti‐IL4 [20 ng/µl; #130 097 742 | #130‐096‐704 | # 130‐095‐709, Miltenyi Biotec, Bergisch Gladbach, Germany]) and for Th2 differentiation [anti‐human CD28 (0.5 µg/ml), IL2 (100 U/ml), IL4 (50 ng/ml), anti‐IFNγ (50 ng/ml), and anti‐IL12 (50 ng/ml; #130 097 742 | #130‐096‐753 | #130 095‐743 | # 130‐103‐738, Miltenyi Biotec, Bergisch Gladbach, Germany)], respectively. After 4 days, the cell suspensions were transferred to an uncovered cell culture plate and incubated for another 2 days with the same supplements except anti‐human CD28.

Hirschberger S., Strauß G., Effinger D., Marstaller X., Ferstl A., Müller M.B., Wu T., Hübner M., Rahmel T., Mascolo H., Exner N., Heß J., Kreth F.W., Unger K, & Kreth S. (2021). Very‐low‐carbohydrate diet enhances human T‐cell immunity through immunometabolic reprogramming. EMBO Molecular Medicine, 13(8), e14323.

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NK Cell Immunophenotyping and IFN-γ Analysis

Cited 1 time

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REAfinity^TM Recombinant Antibodies (Miltenyi Biotec, Bergisch Gladbach, Germany) were used for NK cell monitoring. Anti-CD56-FITC, CD3-PE-Vio770, CD45-VioBlue, CD16-PE were used to identify CD56dim and CD56 bright NK cells. The four stages were discriminated by using CD11b-APC-Vio770 and CD27-APC. ABCC3 expression was assessed before and after each vaccination as previously described [17 (link)], by using a primary antibody anti-ABCC3 (Thermo Fisher Scientific, Waltham, MA, USA) and a secondary anti-rabbit Alexa Fluor488 antibody (Abcam) according to manufacturer’s instructions. PBLs were then fixed and permeabilized using the Cytofix/Cytoperm solution (BD Biosciences, Franlin Lakes, NJ, USA) and intracellularly stained with an anti-IFN-γ (Miltenyi Biotec) antibody. NK cells were gated and then analyzed by flow cytometry for IFN-γ assessment. Acquisition of stained samples was performed using a MACSQuant (Miltenyi Biotec) flow cytometer, and data were analyzed using Flowlogic software (version 7.2, Miltenyi Biotec).

Pellegatta S., Di Ianni N., Pessina S., Paterra R., Anghileri E., Eoli M, & Finocchiaro G. (2019). ABCC3 Expressed by CD56dim CD16+ NK Cells Predicts Response in Glioblastoma Patients Treated with Combined Chemotherapy and Dendritic Cell Immunotherapy. International Journal of Molecular Sciences, 20(23), 5886.

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Stimulation and Intracellular Cytokine Staining

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Total IELs or peripheral blood lymphocytes (PBLs) were stimulated with phorbol 12-myristate 13-acetate (PMA, 25 ng/mL; Sigma-Aldrich, St. Louis, MO) and ionomycin (1 µg/mL; Merck Millipore, Darmstadt, Germany) in the presence of Brefeldin A (10 µg/mL; Sigma-Aldrich) for 4 h at 37°C in a 5% CO 2 atmosphere. Cells were fixed and permeabilized with use of the Inside Stain Kit (Miltenyi Biotec, Gladbach, Germany) and then stained with anti-IFNγ, anti-IL-17A (Miltenyi Biotec), and anti-IL-22 (eBioscience, San Diego, CA) monoclonal antibodies.

Talayero P., Mancebo E., Calvo-Pulido J., Rodríguez-Muñoz S., Bernardo I., Laguna-Goya R., Cano-Romero F.L., García-Sesma A., Loinaz C., Jiménez C., Justo I, & Paz-Artal E. (2016). Innate Lymphoid Cells Groups 1 and 3 in the Epithelial Compartment of Functional Human Intestinal Allografts. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons, 16(1).

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