Immunoprecipitated Flag-TBK1 WT or Flag-TBK1 KD was subject to in vitro kinase assay with 40ng/ul ULK1 active kinase (SignalChem), kinase assay buffer III (SignalChem) and 100μM ATP (SignalChem). After 15min incubation in 30°C water bath, reaction was stopped by adding SDS loading buffer and heating at 95°C. Phospho-TBK1 Ser172 antibody (Cell Signaling) was used to detect phosphorylated TBK1.
Phospho tbk1 ser172 antibody
Manufactured by Cell Signaling Technology
The Phospho-TBK1 Ser172 antibody is a laboratory tool that specifically recognizes the phosphorylated form of the TBK1 protein at serine residue 172. This antibody can be used to detect and analyze the activation state of the TBK1 protein in various experimental systems.
Automatically generated - may contain errors
Lab products found in correlation
Adenosine triphosphate (atp), by Sino Biological (1 mentions)
Anti ucp1 antibody, by Abcam (1 mentions)
Cl 488 conjugated affinipure goat anti rabbit lgg h l, by Proteintech (1 mentions)
Phospho ampkα thr172 antibody, by Cell Signaling Technology (1 mentions)
F4 80 antibody, by Proteintech (1 mentions)
Recombinant murine tnf α, by Thermo Fisher Scientific (1 mentions)
Oil red o solution, by Merck Group (1 mentions)
Bca protein assay kit, by Beyotime (1 mentions)
Gapdh antibody, by Proteintech (1 mentions)
2 protocols using phospho tbk1 ser172 antibody
1
In Vitro TBK1 Kinase Assay
2
Berberine and Ibuprofen Anti-inflammatory Mechanisms
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Berberine chloride dihydrate (Berberine hydrochloride, purity: 98%) was obtained from Yuanye Inc. (Shanghai, China) and Ibuprofen (purity: 98%) was obtained from Aladdin (Shanghai, China). Both of them were used directly after purchase. All other solvents were analytically pure. Deionized water was used in this study. All plasmids were supplied by Shanghai GenePharma Co. Ltd (Shanghai, China). Sources of antibodies were as follows: IKKε antibody (Cell SignalingTechnology, 2690), TBK1 antibody (Cell Signaling Technology, 3013), Phospho-TBK1 Ser172 antibody (Cell Signaling Technology, 5483), Phospho-AMPKα Thr172 antibody (Cell Signaling Technology, 2535), Anti-UCP1 antibody (Abcam, ab10983), F4/80 antibody (proteintech, 28463-1-AP), GAPDH antibody (proteintech, 60004-1-Ig), Anti-rabbit IgG (Cell Signaling Technology, 7074 S), Anti-mouse IgG (Cell Signaling Technology, 7076 S), CL488-conjugated Affinipure Goat Anti-Mouse lgG (H + L) (proteintech, SA00013-1), CL488-conjugated Affinipure Goat Anti-Rabbit lgG (H + L) (proteintech, SA00013-2). 3-Isobutyl-1-methylxanthine (IBMX), dexamethasone (DEX), insulin, ISO and Oil Red O solution (0.5% in isopropanol) were purchased from Sigma-Aldrich (St Louis, MO, USA). Recombinant Murine TNF-α was purchased from Pepro Tech (Rocky Hill, NJ, USA). The BCA protein assay kit was purchased from Beyotime Biotechnology Co., Ltd (Beijing, China).
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