B6.129s1 irf4tm1rdf j

Zbtb46^GFP/+ mice were neomycin cassette-deleted, N8 and N9 backcrosses from 129S6/SvEvTac to C57BL/6J. Batf3^−/− and Irf8^VENUS have been described^{7 (link),44 (link)}. Irf8^−/− mice were generated by crossing Irf8^f/f mice (B6(Cg)-Irf8^tm1.1Hm/J, Jackson Laboratories) with CMV-Cre mice (B6.C-Tg(CMV-cre)1Cgn/J). Irf8^+/− mice were obtained by crossing Irf8^−/− mice to wild-type C57BL/6J.
Irf4^−/− mice were generated by crossing Irf4^f/f mice (B6.129S1-Irf4^tm1Rdf/J, Jackson Laboratories) first to CMV-Cre mice (B6.C-Tg(CMV-cre)1Cgn/J) and then to CMV-Flp1 mice (B6.129S4-Gt(ROSA)26Sor^tm1(FLP1)Dym/JRainJ). BXH2/TyJ mice were from The Jackson Laboratory. All mice were maintained on the C57BL/6J background and maintained in a specific pathogen-free animal facility following institutional guidelines and with protocols approved by the Animal Studies Committee at Washington University in St. Louis. Experiments were performed with mice 8–12 weeks of age using sex-matched littermates.

C57BL/6J, B6.C(Cg)-Cd79a^tm1(cre)Reth/EhobJ (Cd79a^Cre) (41 (link)), B6.129S1-Irf4^tm1Rdf/J (Irf4^frt-fl) (38 (link)), B6.Cg-Tg(ACTFLPe)9205Dym/J (42 (link)), and B6.129P2(C)-Igh^tm2Cgn/J (B1-8i^+/+) (43 (link)) mice were from The Jackson Laboratory. Prdm1^fl mice (39 (link)) were a gift from K. Calame, via D. Allman. Homozygous 2F5 heavy- and light-chain knockin (2F5 KI) mice (44 (link)) were a gift from B. Haynes. Irf4^frt-fl mice were bred with flippase (FLP)-expressing deleter mice (42 (link)) to generate Irf4^+/− mice, and then Irf4^+/− and Irf4^−/− mice were obtained by interbreeding. Irf4^+/− and Irf4^−/− littermates were co-housed and continuously supplied with drinking water containing sulfamethoxazole (1 mg/ml) and trimethoprim (0.025 mg/ml) to control the otherwise high incidence of rectal prolapse in Irf4^−/− mice. All animals were on a C57BL/6 background. All experiments used age- and sex-matched, 8- to 12-week-old male or female mice. Mice were housed under specific-pathogen-free conditions at the Duke University Animal Care Facility. All experiments involving animals were approved by the Duke University Institutional Animal Care and Use Committee.